Strain Fitness in Pseudomonas simiae WCS417 around PS417_17020

Experiment: root sample 6; outgrowth in LB

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPS417_17015 and PS417_17020 are separated by 11 nucleotidesPS417_17020 and PS417_17025 are separated by 61 nucleotidesPS417_17025 and PS417_17030 overlap by 4 nucleotides PS417_17015: PS417_17015 - Isovaleryl-CoA dehydrogenase (EC 1.3.8.4) (from data), at 3,717,572 to 3,718,735 _17015 PS417_17020: PS417_17020 - methylcrotonoyl-CoA carboxylase, at 3,718,747 to 3,720,354 _17020 PS417_17025: PS417_17025 - gamma-carboxygeranoyl-CoA hydratase, at 3,720,416 to 3,721,222 _17025 PS417_17030: PS417_17030 - 3-methylcrotonyl-CoA carboxylase, at 3,721,219 to 3,723,144 _17030 Position (kb) 3718 3719 3720 3721Strain fitness (log2 ratio) -2 -1 0 1 2 3 4 5at 3717.915 kb on - strand, within PS417_17015at 3718.082 kb on + strand, within PS417_17015at 3718.376 kb on + strand, within PS417_17015at 3718.451 kb on + strand, within PS417_17015at 3718.452 kb on - strand, within PS417_17015at 3718.615 kb on + strand, within PS417_17015at 3718.616 kb on - strand, within PS417_17015at 3718.616 kb on - strand, within PS417_17015at 3718.661 kb on + strandat 3719.167 kb on + strand, within PS417_17020at 3719.578 kb on - strand, within PS417_17020at 3720.352 kb on + strandat 3720.352 kb on + strandat 3720.395 kb on + strandat 3720.395 kb on + strandat 3720.396 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction root sample 6; outgrowth in LB
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3,717,915 - PS417_17015 0.29 -0.8
3,718,082 + PS417_17015 0.44 +0.1
3,718,376 + PS417_17015 0.69 +0.2
3,718,451 + PS417_17015 0.76 +1.5
3,718,452 - PS417_17015 0.76 +5.6
3,718,615 + PS417_17015 0.90 -1.5
3,718,616 - PS417_17015 0.90 -2.6
3,718,616 - PS417_17015 0.90 -1.5
3,718,661 + -1.3
3,719,167 + PS417_17020 0.26 +1.0
3,719,578 - PS417_17020 0.52 -1.3
3,720,352 + -0.3
3,720,352 + -1.8
3,720,395 + +1.3
3,720,395 + -0.4
3,720,396 - -0.4

Or see this region's nucleotide sequence