Experiment: root sample 6; outgrowth in LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_17015 and PS417_17020 are separated by 11 nucleotides PS417_17020 and PS417_17025 are separated by 61 nucleotides PS417_17025 and PS417_17030 overlap by 4 nucleotides
PS417_17015: PS417_17015 - Isovaleryl-CoA dehydrogenase (EC 1.3.8.4) (from data) , at 3,717,572 to 3,718,735
_17015
PS417_17020: PS417_17020 - methylcrotonoyl-CoA carboxylase, at 3,718,747 to 3,720,354
_17020
PS417_17025: PS417_17025 - gamma-carboxygeranoyl-CoA hydratase, at 3,720,416 to 3,721,222
_17025
PS417_17030: PS417_17030 - 3-methylcrotonyl-CoA carboxylase, at 3,721,219 to 3,723,144
_17030
Position (kb)
3718
3719
3720
3721 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5 at 3717.915 kb on - strand, within PS417_17015 at 3718.082 kb on + strand, within PS417_17015 at 3718.376 kb on + strand, within PS417_17015 at 3718.451 kb on + strand, within PS417_17015 at 3718.452 kb on - strand, within PS417_17015 at 3718.615 kb on + strand, within PS417_17015 at 3718.616 kb on - strand, within PS417_17015 at 3718.616 kb on - strand, within PS417_17015 at 3718.661 kb on + strand at 3719.167 kb on + strand, within PS417_17020 at 3719.578 kb on - strand, within PS417_17020 at 3720.352 kb on + strand at 3720.352 kb on + strand at 3720.395 kb on + strand at 3720.395 kb on + strand at 3720.396 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction root sample 6; outgrowth in LB remove 3,717,915 - PS417_17015 0.29 -0.8 3,718,082 + PS417_17015 0.44 +0.1 3,718,376 + PS417_17015 0.69 +0.2 3,718,451 + PS417_17015 0.76 +1.5 3,718,452 - PS417_17015 0.76 +5.6 3,718,615 + PS417_17015 0.90 -1.5 3,718,616 - PS417_17015 0.90 -2.6 3,718,616 - PS417_17015 0.90 -1.5 3,718,661 + -1.3 3,719,167 + PS417_17020 0.26 +1.0 3,719,578 - PS417_17020 0.52 -1.3 3,720,352 + -0.3 3,720,352 + -1.8 3,720,395 + +1.3 3,720,395 + -0.4 3,720,396 - -0.4
Or see this region's nucleotide sequence