Experiment: root sample 6; outgrowth in LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_16530 and PS417_16535 are separated by 226 nucleotides PS417_16535 and PS417_16540 are separated by 59 nucleotides PS417_16540 and PS417_16545 overlap by 4 nucleotides PS417_16545 and PS417_16550 are separated by 125 nucleotides
PS417_16530: PS417_16530 - isocitrate dehydrogenase, at 3,611,310 to 3,613,535
_16530
PS417_16535: PS417_16535 - NUDIX hydrolase, at 3,613,762 to 3,614,208
_16535
PS417_16540: PS417_16540 - tRNA 2-thiouridylase, at 3,614,268 to 3,615,392
_16540
PS417_16545: PS417_16545 - DNA repair protein, at 3,615,389 to 3,616,012
_16545
PS417_16550: PS417_16550 - adenylosuccinate lyase, at 3,616,138 to 3,617,508
_16550
Position (kb)
3614
3615
3616 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3613.453 kb on - strand at 3613.533 kb on + strand at 3613.533 kb on + strand at 3613.534 kb on - strand at 3613.541 kb on + strand at 3613.542 kb on - strand at 3613.672 kb on + strand at 3613.673 kb on - strand at 3613.673 kb on - strand at 3613.673 kb on - strand at 3613.673 kb on - strand at 3613.969 kb on + strand, within PS417_16535 at 3613.970 kb on - strand, within PS417_16535 at 3613.970 kb on - strand, within PS417_16535 at 3613.975 kb on + strand, within PS417_16535 at 3614.002 kb on + strand at 3614.050 kb on + strand, within PS417_16535 at 3614.207 kb on - strand at 3614.267 kb on + strand at 3615.514 kb on - strand, within PS417_16545 at 3615.795 kb on - strand, within PS417_16545 at 3615.795 kb on - strand, within PS417_16545 at 3616.019 kb on + strand at 3616.019 kb on + strand at 3616.092 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction root sample 6; outgrowth in LB remove 3,613,453 - +1.3 3,613,533 + -1.3 3,613,533 + -1.4 3,613,534 - -0.5 3,613,541 + -0.2 3,613,542 - +1.8 3,613,672 + -0.2 3,613,673 - -1.8 3,613,673 - -0.3 3,613,673 - -1.1 3,613,673 - -0.3 3,613,969 + PS417_16535 0.46 +1.4 3,613,970 - PS417_16535 0.47 +0.5 3,613,970 - PS417_16535 0.47 -0.4 3,613,975 + PS417_16535 0.48 -0.3 3,614,002 + +1.5 3,614,050 + PS417_16535 0.64 -0.0 3,614,207 - +1.7 3,614,267 + +0.1 3,615,514 - PS417_16545 0.20 +3.1 3,615,795 - PS417_16545 0.65 -0.2 3,615,795 - PS417_16545 0.65 -0.6 3,616,019 + +0.7 3,616,019 + -0.0 3,616,092 + +1.9
Or see this region's nucleotide sequence