Experiment: root sample 6; outgrowth in LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_12870 and PS417_12875 are separated by 5 nucleotides PS417_12875 and PS417_12880 overlap by 33 nucleotides PS417_12880 and PS417_12885 are separated by 176 nucleotides
PS417_12870: PS417_12870 - enoyl-CoA hydratase, at 2,815,186 to 2,815,998
_12870
PS417_12875: PS417_12875 - NADH pyrophosphatase, at 2,816,004 to 2,816,837
_12875
PS417_12880: PS417_12880 - membrane protein, at 2,816,805 to 2,817,584
_12880
PS417_12885: PS417_12885 - phosphatidylcholine synthase, at 2,817,761 to 2,818,480
_12885
Position (kb)
2816
2817
2818 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2815.865 kb on - strand, within PS417_12870 at 2815.900 kb on + strand, within PS417_12870 at 2815.923 kb on + strand at 2816.298 kb on + strand, within PS417_12875 at 2816.391 kb on + strand, within PS417_12875 at 2816.392 kb on - strand, within PS417_12875 at 2816.509 kb on - strand, within PS417_12875 at 2816.509 kb on - strand, within PS417_12875 at 2816.509 kb on - strand, within PS417_12875 at 2816.509 kb on - strand, within PS417_12875 at 2816.590 kb on + strand, within PS417_12875 at 2816.604 kb on - strand, within PS417_12875 at 2816.610 kb on + strand, within PS417_12875 at 2816.610 kb on + strand, within PS417_12875 at 2816.610 kb on + strand at 2816.611 kb on - strand, within PS417_12875 at 2816.611 kb on - strand, within PS417_12875 at 2816.611 kb on - strand, within PS417_12875 at 2816.611 kb on - strand, within PS417_12875 at 2816.791 kb on - strand at 2817.227 kb on - strand, within PS417_12880 at 2817.564 kb on + strand at 2817.595 kb on + strand at 2817.595 kb on + strand at 2817.596 kb on - strand at 2817.690 kb on + strand at 2817.691 kb on - strand at 2817.806 kb on - strand at 2817.822 kb on + strand at 2817.823 kb on - strand at 2817.925 kb on + strand, within PS417_12885 at 2818.042 kb on + strand, within PS417_12885 at 2818.042 kb on + strand, within PS417_12885 at 2818.044 kb on + strand at 2818.044 kb on + strand, within PS417_12885 at 2818.045 kb on - strand, within PS417_12885 at 2818.045 kb on - strand, within PS417_12885 at 2818.194 kb on + strand, within PS417_12885 at 2818.223 kb on + strand, within PS417_12885 at 2818.223 kb on + strand, within PS417_12885 at 2818.223 kb on + strand, within PS417_12885 at 2818.224 kb on - strand, within PS417_12885 at 2818.224 kb on - strand, within PS417_12885 at 2818.224 kb on - strand, within PS417_12885 at 2818.227 kb on + strand, within PS417_12885 at 2818.227 kb on + strand, within PS417_12885 at 2818.228 kb on - strand, within PS417_12885 at 2818.228 kb on - strand, within PS417_12885 at 2818.237 kb on - strand at 2818.410 kb on - strand at 2818.427 kb on - strand at 2818.460 kb on + strand at 2818.461 kb on - strand at 2818.475 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction root sample 6; outgrowth in LB remove 2,815,865 - PS417_12870 0.84 -0.2 2,815,900 + PS417_12870 0.88 +1.1 2,815,923 + -0.1 2,816,298 + PS417_12875 0.35 -0.2 2,816,391 + PS417_12875 0.46 -0.5 2,816,392 - PS417_12875 0.47 -0.3 2,816,509 - PS417_12875 0.61 +0.4 2,816,509 - PS417_12875 0.61 -2.5 2,816,509 - PS417_12875 0.61 -0.0 2,816,509 - PS417_12875 0.61 -0.4 2,816,590 + PS417_12875 0.70 -0.6 2,816,604 - PS417_12875 0.72 -0.2 2,816,610 + PS417_12875 0.73 +0.4 2,816,610 + PS417_12875 0.73 -1.9 2,816,610 + +1.5 2,816,611 - PS417_12875 0.73 -0.0 2,816,611 - PS417_12875 0.73 -1.5 2,816,611 - PS417_12875 0.73 -0.5 2,816,611 - PS417_12875 0.73 +0.3 2,816,791 - +0.6 2,817,227 - PS417_12880 0.54 -0.0 2,817,564 + +1.0 2,817,595 + -0.6 2,817,595 + +1.7 2,817,596 - +0.3 2,817,690 + -1.5 2,817,691 - +1.2 2,817,806 - +0.7 2,817,822 + +0.4 2,817,823 - +1.2 2,817,925 + PS417_12885 0.23 -0.7 2,818,042 + PS417_12885 0.39 -0.4 2,818,042 + PS417_12885 0.39 +0.5 2,818,044 + +0.9 2,818,044 + PS417_12885 0.39 -1.0 2,818,045 - PS417_12885 0.39 -1.7 2,818,045 - PS417_12885 0.39 -0.5 2,818,194 + PS417_12885 0.60 -0.1 2,818,223 + PS417_12885 0.64 -1.4 2,818,223 + PS417_12885 0.64 -0.2 2,818,223 + PS417_12885 0.64 -1.9 2,818,224 - PS417_12885 0.64 +0.3 2,818,224 - PS417_12885 0.64 -0.3 2,818,224 - PS417_12885 0.64 +1.5 2,818,227 + PS417_12885 0.65 -0.3 2,818,227 + PS417_12885 0.65 +0.1 2,818,228 - PS417_12885 0.65 -2.1 2,818,228 - PS417_12885 0.65 +0.5 2,818,237 - +0.7 2,818,410 - -2.6 2,818,427 - -0.7 2,818,460 + -1.0 2,818,461 - -0.5 2,818,475 + +1.8
Or see this region's nucleotide sequence