Experiment: root sample 6; outgrowth in LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_12215 and PS417_12220 are separated by 98 nucleotides PS417_12220 and PS417_12225 are separated by 65 nucleotides PS417_12225 and PS417_12230 are separated by 30 nucleotides PS417_12230 and PS417_12235 are separated by 64 nucleotides
PS417_12215: PS417_12215 - lytic transglycosylase, at 2,655,400 to 2,656,815
_12215
PS417_12220: PS417_12220 - hydroxyacylglutathione hydrolase, at 2,656,914 to 2,657,681
_12220
PS417_12225: PS417_12225 - SAM-dependent methyltransferase, at 2,657,747 to 2,658,505
_12225
PS417_12230: PS417_12230 - ribonuclease H, at 2,658,536 to 2,658,988
_12230
PS417_12235: PS417_12235 - DNA polymerase III subunit epsilon, at 2,659,053 to 2,659,799
_12235
Position (kb)
2657
2658
2659 Strain fitness (log2 ratio)
-2
-1
0
1 at 2656.894 kb on - strand at 2657.323 kb on + strand, within PS417_12220 at 2657.360 kb on + strand, within PS417_12220 at 2657.360 kb on + strand, within PS417_12220 at 2657.362 kb on + strand, within PS417_12220 at 2657.628 kb on + strand at 2657.642 kb on - strand at 2657.642 kb on - strand at 2657.676 kb on + strand at 2657.677 kb on - strand at 2657.677 kb on - strand at 2657.718 kb on + strand at 2657.718 kb on + strand at 2659.004 kb on + strand at 2659.007 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction root sample 6; outgrowth in LB remove 2,656,894 - +0.6 2,657,323 + PS417_12220 0.53 +0.5 2,657,360 + PS417_12220 0.58 -1.3 2,657,360 + PS417_12220 0.58 -2.0 2,657,362 + PS417_12220 0.58 +0.5 2,657,628 + +1.0 2,657,642 - -1.7 2,657,642 - -0.7 2,657,676 + -0.2 2,657,677 - -0.2 2,657,677 - +0.1 2,657,718 + +0.9 2,657,718 + +0.3 2,659,004 + -0.4 2,659,007 + -1.0
Or see this region's nucleotide sequence