Experiment: root sample 6; outgrowth in LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_11975 and PS417_11980 are separated by 250 nucleotides PS417_11980 and PS417_11990 are separated by 350 nucleotides PS417_11990 and PS417_11995 are separated by 15 nucleotides PS417_11995 and PS417_12000 overlap by 4 nucleotides
PS417_11975: PS417_11975 - diguanylate cyclase, at 2,605,882 to 2,606,826
_11975
PS417_11980: PS417_11980 - hypothetical protein, at 2,607,077 to 2,607,325
_11980
PS417_11990: PS417_11990 - hypothetical protein, at 2,607,676 to 2,607,987
_11990
PS417_11995: PS417_11995 - RNA polymerase sigma factor, at 2,608,003 to 2,608,581
_11995
PS417_12000: PS417_12000 - hypothetical protein, at 2,608,578 to 2,609,009
_12000
Position (kb)
2607
2608 Strain fitness (log2 ratio)
-2
-1
0
1 at 2606.717 kb on + strand, within PS417_11975 at 2606.718 kb on - strand, within PS417_11975 at 2606.718 kb on - strand, within PS417_11975 at 2606.718 kb on - strand at 2606.718 kb on - strand, within PS417_11975 at 2606.718 kb on - strand, within PS417_11975 at 2606.786 kb on + strand at 2606.786 kb on + strand at 2606.787 kb on - strand at 2607.024 kb on + strand at 2607.024 kb on + strand at 2607.025 kb on - strand at 2607.025 kb on - strand at 2607.025 kb on - strand at 2607.025 kb on - strand at 2607.025 kb on - strand at 2607.025 kb on - strand at 2607.365 kb on + strand at 2607.365 kb on + strand at 2607.446 kb on + strand at 2607.587 kb on - strand at 2608.037 kb on + strand at 2608.038 kb on - strand at 2608.038 kb on - strand at 2608.429 kb on + strand, within PS417_11995
Per-strain Table
Position Strand Gene LocusTag Fraction root sample 6; outgrowth in LB remove 2,606,717 + PS417_11975 0.88 +0.2 2,606,718 - PS417_11975 0.88 -0.2 2,606,718 - PS417_11975 0.88 +0.1 2,606,718 - +1.1 2,606,718 - PS417_11975 0.88 +1.3 2,606,718 - PS417_11975 0.88 -0.1 2,606,786 + -2.3 2,606,786 + +0.4 2,606,787 - +0.3 2,607,024 + +0.8 2,607,024 + -1.1 2,607,025 - +0.3 2,607,025 - +1.3 2,607,025 - -0.7 2,607,025 - -1.4 2,607,025 - +0.1 2,607,025 - -1.6 2,607,365 + -0.6 2,607,365 + -0.4 2,607,446 + -1.6 2,607,587 - -2.0 2,608,037 + +0.4 2,608,038 - +0.4 2,608,038 - -0.6 2,608,429 + PS417_11995 0.74 -0.5
Or see this region's nucleotide sequence