Experiment: root sample 6; outgrowth in LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_11240 and PS417_11245 overlap by 4 nucleotides PS417_11245 and PS417_11250 are separated by 37 nucleotides PS417_11250 and PS417_11255 overlap by 4 nucleotides PS417_11255 and PS417_11260 overlap by 4 nucleotides
PS417_11240: PS417_11240 - VreA, at 2,409,694 to 2,410,338
_11240
PS417_11245: PS417_11245 - RNA polymerase subunit sigma-24, at 2,410,335 to 2,410,880
_11245
PS417_11250: PS417_11250 - iron dicitrate transport regulator FecR, at 2,410,918 to 2,411,844
_11250
PS417_11255: PS417_11255 - general secretion pathway protein GspJ, at 2,411,841 to 2,412,452
_11255
PS417_11260: PS417_11260 - general secretion pathway protein GspH, at 2,412,449 to 2,412,901
_11260
Position (kb)
2410
2411
2412 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2409.932 kb on - strand at 2410.383 kb on + strand at 2410.459 kb on - strand, within PS417_11245 at 2410.680 kb on + strand, within PS417_11245 at 2410.681 kb on - strand, within PS417_11245 at 2410.779 kb on - strand, within PS417_11245 at 2410.779 kb on - strand, within PS417_11245 at 2410.878 kb on + strand at 2412.420 kb on + strand at 2412.421 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction root sample 6; outgrowth in LB remove 2,409,932 - +1.8 2,410,383 + -2.3 2,410,459 - PS417_11245 0.23 -0.6 2,410,680 + PS417_11245 0.63 -0.9 2,410,681 - PS417_11245 0.63 -0.8 2,410,779 - PS417_11245 0.81 -0.4 2,410,779 - PS417_11245 0.81 -0.1 2,410,878 + +0.4 2,412,420 + -0.5 2,412,421 - -2.0
Or see this region's nucleotide sequence