Experiment: root sample 6; outgrowth in LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_10420 and PS417_10425 are separated by 57 nucleotides PS417_10425 and PS417_10430 are separated by 115 nucleotides PS417_10430 and PS417_10435 are separated by 69 nucleotides
PS417_10420: PS417_10420 - shikimate dehydrogenase, at 2,226,422 to 2,227,240
_10420
PS417_10425: PS417_10425 - hypothetical protein, at 2,227,298 to 2,227,945
_10425
PS417_10430: PS417_10430 - hypothetical protein, at 2,228,061 to 2,228,570
_10430
PS417_10435: PS417_10435 - serine/threonine protein kinase, at 2,228,640 to 2,229,860
_10435
Position (kb)
2228
2229 Strain fitness (log2 ratio)
-2
-1
0
1 at 2227.178 kb on + strand at 2227.178 kb on + strand at 2227.179 kb on - strand at 2227.458 kb on + strand, within PS417_10425 at 2227.840 kb on - strand, within PS417_10425 at 2227.957 kb on + strand at 2227.958 kb on - strand at 2227.979 kb on - strand at 2228.066 kb on + strand at 2228.296 kb on - strand, within PS417_10430 at 2228.304 kb on - strand, within PS417_10430 at 2228.554 kb on - strand at 2228.554 kb on - strand at 2228.554 kb on - strand at 2228.569 kb on - strand at 2228.689 kb on - strand at 2228.761 kb on - strand at 2229.004 kb on + strand, within PS417_10435 at 2229.568 kb on - strand, within PS417_10435
Per-strain Table
Position Strand Gene LocusTag Fraction root sample 6; outgrowth in LB remove 2,227,178 + +1.3 2,227,178 + -1.6 2,227,179 - -0.8 2,227,458 + PS417_10425 0.25 -0.8 2,227,840 - PS417_10425 0.84 -2.0 2,227,957 + -0.4 2,227,958 - +0.8 2,227,979 - -0.8 2,228,066 + +0.4 2,228,296 - PS417_10430 0.46 -0.5 2,228,304 - PS417_10430 0.48 -0.5 2,228,554 - +0.8 2,228,554 - +0.1 2,228,554 - -0.6 2,228,569 - -0.8 2,228,689 - -1.1 2,228,761 - -0.7 2,229,004 + PS417_10435 0.30 -0.7 2,229,568 - PS417_10435 0.76 -0.5
Or see this region's nucleotide sequence