Strain Fitness in Escherichia coli BW25113 around b4547

Experiment: LB Aerobic with Sulfamonomethoxine 0.000125 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntdapE and ypfN are separated by 27 nucleotidesypfN and ypfH are separated by 109 nucleotidesypfH and ypfI are separated by 73 nucleotides b2472: dapE - succinyl-diaminopimelate desuccinylase (NCBI), at 2,589,629 to 2,590,756 dapE b4547: ypfN - hypothetical protein (NCBI), at 2,590,784 to 2,590,984 ypfN b2473: ypfH - orf, hypothetical protein (VIMSS), at 2,591,094 to 2,591,792 ypfH b2474: ypfI - predicted hydrolase (NCBI), at 2,591,866 to 2,593,881 ypfI Position (kb) 2590 2591Strain fitness (log2 ratio) -1 0 1at 2590.852 kb on + strand, within ypfNat 2590.852 kb on + strand, within ypfNat 2590.934 kb on + strand, within ypfNat 2590.934 kb on + strand, within ypfNat 2590.954 kb on - strand, within ypfNat 2590.954 kb on - strand, within ypfNat 2590.997 kb on + strandat 2591.119 kb on - strandat 2591.119 kb on - strandat 2591.127 kb on - strandat 2591.178 kb on - strand, within ypfHat 2591.178 kb on - strand, within ypfHat 2591.358 kb on - strand, within ypfHat 2591.372 kb on + strand, within ypfHat 2591.372 kb on + strand, within ypfHat 2591.380 kb on - strand, within ypfHat 2591.489 kb on + strand, within ypfHat 2591.494 kb on + strand, within ypfHat 2591.584 kb on - strand, within ypfHat 2591.584 kb on - strand, within ypfHat 2591.680 kb on - strand, within ypfHat 2591.720 kb on - strand, within ypfHat 2591.720 kb on - strand, within ypfHat 2591.752 kb on + strandat 2591.912 kb on - strandat 2591.978 kb on - strandat 2591.978 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Aerobic with Sulfamonomethoxine 0.000125 mM
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2,590,852 + ypfN b4547 0.34 +0.3
2,590,852 + ypfN b4547 0.34 -0.1
2,590,934 + ypfN b4547 0.75 +0.0
2,590,934 + ypfN b4547 0.75 +0.8
2,590,954 - ypfN b4547 0.85 -0.5
2,590,954 - ypfN b4547 0.85 +1.0
2,590,997 + +0.6
2,591,119 - +0.8
2,591,119 - -1.2
2,591,127 - +0.0
2,591,178 - ypfH b2473 0.12 -0.5
2,591,178 - ypfH b2473 0.12 -0.6
2,591,358 - ypfH b2473 0.38 -0.3
2,591,372 + ypfH b2473 0.40 +1.2
2,591,372 + ypfH b2473 0.40 -0.1
2,591,380 - ypfH b2473 0.41 -1.4
2,591,489 + ypfH b2473 0.57 +0.2
2,591,494 + ypfH b2473 0.57 -0.1
2,591,584 - ypfH b2473 0.70 -0.1
2,591,584 - ypfH b2473 0.70 +0.9
2,591,680 - ypfH b2473 0.84 +0.7
2,591,720 - ypfH b2473 0.90 -0.2
2,591,720 - ypfH b2473 0.90 -0.4
2,591,752 + -0.4
2,591,912 - +0.1
2,591,978 - -0.2
2,591,978 - -0.2

Or see this region's nucleotide sequence