Strain Fitness in Escherichia coli BW25113 around b2300

Experiment: LB Aerobic with Sulfamonomethoxine 0.000125 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyfcC and yfcD are separated by 32 nucleotidesyfcD and yfcE are separated by 57 nucleotidesyfcE and yfcF are separated by 52 nucleotidesyfcF and yfcG are separated by 135 nucleotides b2298: yfcC - putative S-transferase (VIMSS), at 2,415,103 to 2,416,623 yfcC b2299: yfcD - predicted NUDIX hydrolase (NCBI), at 2,416,656 to 2,417,198 yfcD b2300: yfcE - orf, hypothetical protein (VIMSS), at 2,417,256 to 2,417,810 yfcE b2301: yfcF - predicted enzyme (NCBI), at 2,417,863 to 2,418,507 yfcF b2302: yfcG - predicted glutathione S-transferase (NCBI), at 2,418,643 to 2,419,290 yfcG Position (kb) 2417 2418Strain fitness (log2 ratio) -2 -1 0 1at 2416.315 kb on - strand, within yfcCat 2416.315 kb on - strand, within yfcCat 2416.355 kb on + strand, within yfcCat 2416.358 kb on - strand, within yfcCat 2416.358 kb on - strand, within yfcCat 2416.420 kb on - strand, within yfcCat 2416.456 kb on + strand, within yfcCat 2416.475 kb on + strandat 2416.502 kb on + strandat 2416.507 kb on + strandat 2416.512 kb on + strandat 2416.512 kb on + strandat 2416.516 kb on - strandat 2416.532 kb on - strandat 2416.532 kb on - strandat 2416.552 kb on + strandat 2416.605 kb on + strandat 2416.606 kb on - strandat 2416.609 kb on + strandat 2416.736 kb on + strand, within yfcDat 2416.811 kb on - strand, within yfcDat 2416.829 kb on + strand, within yfcDat 2416.973 kb on + strand, within yfcDat 2416.977 kb on - strand, within yfcDat 2416.977 kb on - strand, within yfcDat 2416.977 kb on - strand, within yfcDat 2417.064 kb on + strand, within yfcDat 2417.124 kb on + strand, within yfcDat 2417.124 kb on + strand, within yfcDat 2417.162 kb on - strandat 2417.177 kb on + strandat 2417.225 kb on + strandat 2417.235 kb on - strandat 2417.408 kb on + strand, within yfcEat 2417.730 kb on + strand, within yfcEat 2417.745 kb on + strand, within yfcEat 2417.745 kb on + strand, within yfcEat 2417.753 kb on - strand, within yfcEat 2417.753 kb on - strand, within yfcEat 2417.759 kb on - strandat 2417.965 kb on + strand, within yfcFat 2417.965 kb on + strand, within yfcFat 2417.970 kb on - strand, within yfcFat 2417.970 kb on - strand, within yfcFat 2418.048 kb on - strand, within yfcFat 2418.133 kb on + strand, within yfcFat 2418.153 kb on + strand, within yfcFat 2418.184 kb on - strand, within yfcFat 2418.247 kb on + strand, within yfcFat 2418.247 kb on + strand, within yfcFat 2418.269 kb on + strand, within yfcFat 2418.269 kb on + strand, within yfcFat 2418.445 kb on - strandat 2418.572 kb on - strandat 2418.607 kb on - strandat 2418.607 kb on - strandat 2418.607 kb on - strandat 2418.607 kb on - strandat 2418.714 kb on + strand, within yfcGat 2418.736 kb on + strand, within yfcGat 2418.739 kb on - strand, within yfcGat 2418.739 kb on - strand, within yfcGat 2418.744 kb on - strand, within yfcG

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Aerobic with Sulfamonomethoxine 0.000125 mM
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2,416,315 - yfcC b2298 0.80 -0.6
2,416,315 - yfcC b2298 0.80 -0.0
2,416,355 + yfcC b2298 0.82 +0.2
2,416,358 - yfcC b2298 0.83 +1.0
2,416,358 - yfcC b2298 0.83 -0.4
2,416,420 - yfcC b2298 0.87 -0.8
2,416,456 + yfcC b2298 0.89 -0.2
2,416,475 + +0.1
2,416,502 + -0.3
2,416,507 + +0.2
2,416,512 + -0.1
2,416,512 + -0.5
2,416,516 - -0.1
2,416,532 - -0.1
2,416,532 - +0.7
2,416,552 + -0.0
2,416,605 + -1.1
2,416,606 - +0.1
2,416,609 + -1.1
2,416,736 + yfcD b2299 0.15 -0.4
2,416,811 - yfcD b2299 0.29 -0.8
2,416,829 + yfcD b2299 0.32 +0.3
2,416,973 + yfcD b2299 0.58 +0.2
2,416,977 - yfcD b2299 0.59 +1.6
2,416,977 - yfcD b2299 0.59 -0.9
2,416,977 - yfcD b2299 0.59 +0.2
2,417,064 + yfcD b2299 0.75 -0.2
2,417,124 + yfcD b2299 0.86 -0.2
2,417,124 + yfcD b2299 0.86 -0.1
2,417,162 - -0.1
2,417,177 + +0.1
2,417,225 + -0.1
2,417,235 - -0.5
2,417,408 + yfcE b2300 0.27 -0.6
2,417,730 + yfcE b2300 0.85 +0.2
2,417,745 + yfcE b2300 0.88 +1.0
2,417,745 + yfcE b2300 0.88 +1.0
2,417,753 - yfcE b2300 0.90 +0.4
2,417,753 - yfcE b2300 0.90 -0.1
2,417,759 - +0.5
2,417,965 + yfcF b2301 0.16 +0.0
2,417,965 + yfcF b2301 0.16 +0.9
2,417,970 - yfcF b2301 0.17 +1.5
2,417,970 - yfcF b2301 0.17 +0.9
2,418,048 - yfcF b2301 0.29 +0.0
2,418,133 + yfcF b2301 0.42 +0.2
2,418,153 + yfcF b2301 0.45 +0.5
2,418,184 - yfcF b2301 0.50 -0.5
2,418,247 + yfcF b2301 0.60 +0.2
2,418,247 + yfcF b2301 0.60 -0.6
2,418,269 + yfcF b2301 0.63 -0.3
2,418,269 + yfcF b2301 0.63 +0.0
2,418,445 - -0.5
2,418,572 - -0.6
2,418,607 - -1.9
2,418,607 - -0.3
2,418,607 - +0.1
2,418,607 - +0.5
2,418,714 + yfcG b2302 0.11 -0.1
2,418,736 + yfcG b2302 0.14 -0.1
2,418,739 - yfcG b2302 0.15 +0.4
2,418,739 - yfcG b2302 0.15 -0.0
2,418,744 - yfcG b2302 0.16 -0.1

Or see this region's nucleotide sequence