Strain Fitness in Escherichia coli BW25113 around b1699

Experiment: LB Aerobic with Sulfamonomethoxine 0.000125 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntydiR and ydiS are separated by 55 nucleotidesydiS and ydiT overlap by 4 nucleotidesydiT and fadK are separated by 56 nucleotides b1698: ydiR - predicted electron transfer flavoprotein, FAD-binding (NCBI), at 1,778,425 to 1,779,363 ydiR b1699: ydiS - predicted oxidoreductase with FAD/NAD(P)-binding domain (NCBI), at 1,779,419 to 1,780,708 ydiS b1700: ydiT - predicted 4Fe-4S ferredoxin-type protein (NCBI), at 1,780,705 to 1,780,998 ydiT b1701: fadK - short chain acyl-CoA synthetase, anaerobic (RefSeq), at 1,781,055 to 1,782,701 fadK Position (kb) 1779 1780 1781Strain fitness (log2 ratio) -1 0 1at 1778.469 kb on + strandat 1778.740 kb on + strand, within ydiRat 1778.933 kb on - strand, within ydiRat 1779.145 kb on + strand, within ydiRat 1779.222 kb on - strand, within ydiRat 1779.423 kb on - strandat 1779.424 kb on - strandat 1779.424 kb on - strandat 1779.484 kb on + strandat 1779.721 kb on + strand, within ydiSat 1779.847 kb on + strand, within ydiSat 1780.127 kb on - strand, within ydiSat 1780.378 kb on - strand, within ydiSat 1780.483 kb on - strand, within ydiSat 1780.483 kb on - strand, within ydiSat 1780.566 kb on - strand, within ydiSat 1780.580 kb on + strandat 1780.598 kb on + strandat 1780.939 kb on + strand, within ydiTat 1781.119 kb on - strandat 1781.209 kb on - strandat 1781.217 kb on - strandat 1781.266 kb on + strand, within fadKat 1781.399 kb on - strand, within fadKat 1781.530 kb on + strand, within fadK

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Aerobic with Sulfamonomethoxine 0.000125 mM
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1,778,469 + -0.4
1,778,740 + ydiR b1698 0.34 -0.3
1,778,933 - ydiR b1698 0.54 -1.2
1,779,145 + ydiR b1698 0.77 +1.3
1,779,222 - ydiR b1698 0.85 -1.1
1,779,423 - -0.1
1,779,424 - -1.7
1,779,424 - -1.0
1,779,484 + -0.5
1,779,721 + ydiS b1699 0.23 +0.8
1,779,847 + ydiS b1699 0.33 +0.3
1,780,127 - ydiS b1699 0.55 +0.2
1,780,378 - ydiS b1699 0.74 +0.6
1,780,483 - ydiS b1699 0.82 +0.6
1,780,483 - ydiS b1699 0.82 +0.2
1,780,566 - ydiS b1699 0.89 -0.1
1,780,580 + -1.6
1,780,598 + +0.4
1,780,939 + ydiT b1700 0.80 -1.1
1,781,119 - +0.1
1,781,209 - +0.1
1,781,217 - +0.5
1,781,266 + fadK b1701 0.13 -0.8
1,781,399 - fadK b1701 0.21 -1.3
1,781,530 + fadK b1701 0.29 -0.1

Or see this region's nucleotide sequence