Experiment: LB Aerobic with Sulfamonomethoxine 0.000125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rem and hokD are separated by 216 nucleotides hokD and relE are separated by 71 nucleotides relE and relB overlap by 1 nucleotides relB and ydfV are separated by 24 nucleotides ydfV and flxA are separated by 202 nucleotides flxA and intK are separated by 436 nucleotides
b1561: rem - Qin prophage; predicted protein (NCBI), at 1,642,675 to 1,642,926
rem
b1562: hokD - Qin prophage; small toxic polypeptide (NCBI), at 1,643,143 to 1,643,298
hokD
b1563: relE - Qin prophage; toxin of the RelE-RelB toxin-antitoxin system (NCBI), at 1,643,370 to 1,643,657
relE
b1564: relB - Qin prophage; bifunctional antitoxin of the RelE-RelB toxin-antitoxin system/ transcriptional repressor (NCBI), at 1,643,657 to 1,643,896
relB
b1565: ydfV - Qin prophage; predicted protein (NCBI), at 1,643,921 to 1,644,226
ydfV
b1566: flxA - Qin prophage; predicted protein (NCBI), at 1,644,429 to 1,644,761
flxA
b1567: intK - Qin prophage; predicted protein (RefSeq), at 1,645,198 to 1,645,380
intK
Position (kb)
1643
1644
1645 Strain fitness (log2 ratio)
-2
-1
0
1 at 1643.141 kb on + strand at 1643.261 kb on - strand, within hokD at 1643.463 kb on - strand, within relE at 1643.463 kb on - strand, within relE at 1643.482 kb on + strand, within relE at 1643.661 kb on + strand at 1643.947 kb on - strand at 1643.963 kb on + strand, within ydfV at 1643.963 kb on + strand, within ydfV at 1643.963 kb on + strand, within ydfV at 1644.038 kb on - strand, within ydfV at 1644.041 kb on + strand, within ydfV at 1644.049 kb on - strand, within ydfV at 1644.187 kb on - strand, within ydfV at 1644.187 kb on - strand, within ydfV at 1644.248 kb on - strand at 1644.258 kb on - strand at 1644.283 kb on + strand at 1644.290 kb on + strand at 1644.306 kb on + strand at 1644.480 kb on + strand, within flxA at 1644.647 kb on + strand, within flxA at 1644.647 kb on + strand, within flxA at 1644.656 kb on + strand, within flxA at 1644.690 kb on + strand, within flxA at 1644.756 kb on + strand at 1644.756 kb on + strand at 1644.758 kb on + strand at 1644.774 kb on + strand at 1644.787 kb on - strand at 1644.792 kb on - strand at 1644.809 kb on - strand at 1644.809 kb on - strand at 1644.870 kb on - strand at 1644.870 kb on - strand at 1644.870 kb on - strand at 1644.890 kb on - strand at 1644.918 kb on - strand at 1644.986 kb on - strand at 1645.019 kb on + strand at 1645.019 kb on + strand at 1645.027 kb on - strand at 1645.027 kb on - strand at 1645.038 kb on - strand at 1645.060 kb on + strand at 1645.062 kb on + strand at 1645.087 kb on + strand at 1645.116 kb on - strand at 1645.144 kb on + strand at 1645.145 kb on + strand at 1645.174 kb on - strand at 1645.176 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB Aerobic with Sulfamonomethoxine 0.000125 mM remove 1,643,141 + -0.8 1,643,261 - hokD b1562 0.76 -0.5 1,643,463 - relE b1563 0.32 +0.2 1,643,463 - relE b1563 0.32 +0.3 1,643,482 + relE b1563 0.39 -0.4 1,643,661 + +0.1 1,643,947 - +0.8 1,643,963 + ydfV b1565 0.14 +0.6 1,643,963 + ydfV b1565 0.14 -0.2 1,643,963 + ydfV b1565 0.14 +0.4 1,644,038 - ydfV b1565 0.38 +1.0 1,644,041 + ydfV b1565 0.39 -1.0 1,644,049 - ydfV b1565 0.42 -0.0 1,644,187 - ydfV b1565 0.87 +0.5 1,644,187 - ydfV b1565 0.87 +1.2 1,644,248 - +1.2 1,644,258 - +1.2 1,644,283 + -0.4 1,644,290 + -0.3 1,644,306 + +0.5 1,644,480 + flxA b1566 0.15 -0.2 1,644,647 + flxA b1566 0.65 -1.2 1,644,647 + flxA b1566 0.65 -1.4 1,644,656 + flxA b1566 0.68 -2.1 1,644,690 + flxA b1566 0.78 -0.7 1,644,756 + +1.2 1,644,756 + +0.6 1,644,758 + -0.3 1,644,774 + -1.8 1,644,787 - +1.2 1,644,792 - +1.2 1,644,809 - -0.8 1,644,809 - +1.2 1,644,870 - -0.1 1,644,870 - +1.2 1,644,870 - +0.9 1,644,890 - +0.0 1,644,918 - -0.2 1,644,986 - -0.0 1,645,019 + +1.2 1,645,019 + +1.2 1,645,027 - -0.2 1,645,027 - +0.2 1,645,038 - -0.0 1,645,060 + -0.7 1,645,062 + -0.4 1,645,087 + +0.9 1,645,116 - -0.0 1,645,144 + -0.0 1,645,145 + +0.8 1,645,174 - +0.1 1,645,176 - -0.2
Or see this region's nucleotide sequence