Strain Fitness in Escherichia coli BW25113 around b1213

Experiment: LB Aerobic with Sulfamonomethoxine 0.000125 mM

Add experiment(s):

Zoom: Pan:

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

download SVG
500 ntprfA and prmC overlap by 1 nucleotidesprmC and ychQ overlap by 4 nucleotidesychQ and ychA are separated by 3 nucleotidesychA and kdsA are separated by 35 nucleotides b1211: prfA - peptide chain release factor 1 (NCBI), at 1,264,235 to 1,265,317 prfA b1212: prmC - N5-glutamine S-adenosyl-L-methionine-dependent methyltransferase (NCBI), at 1,265,317 to 1,266,150 prmC b1213: ychQ - predicted transcriptional regulator (NCBI), at 1,266,147 to 1,266,539 ychQ b1214: ychA - predicted transcriptional regulator (NCBI), at 1,266,543 to 1,267,352 ychA b1215: kdsA - 2-dehydro-3-deoxyphosphooctonate aldolase (NCBI), at 1,267,388 to 1,268,242 kdsA Position (kb) 1266 1267Strain fitness (log2 ratio) -1 0 1 2at 1266.145 kb on - strandat 1266.167 kb on + strandat 1266.172 kb on + strandat 1266.240 kb on - strand, within ychQat 1266.325 kb on + strand, within ychQat 1266.325 kb on + strand, within ychQat 1266.435 kb on - strand, within ychQat 1266.483 kb on + strand, within ychQat 1266.522 kb on - strandat 1266.644 kb on - strand, within ychAat 1266.701 kb on + strand, within ychAat 1266.737 kb on + strand, within ychAat 1266.990 kb on + strand, within ychAat 1267.094 kb on + strand, within ychAat 1267.104 kb on - strand, within ychAat 1267.139 kb on + strand, within ychAat 1267.242 kb on + strand, within ychAat 1267.242 kb on + strand, within ychAat 1267.242 kb on + strand, within ychAat 1267.274 kb on + strandat 1267.274 kb on + strandat 1267.274 kb on - strandat 1267.276 kb on + strandat 1267.276 kb on + strand

download strain data

Per-strain Table

Position Strand Gene LocusTag Fraction LB Aerobic with Sulfamonomethoxine 0.000125 mM
remove
1,266,145 - -0.3
1,266,167 + -0.2
1,266,172 + +0.2
1,266,240 - ychQ b1213 0.24 -0.5
1,266,325 + ychQ b1213 0.45 +0.7
1,266,325 + ychQ b1213 0.45 +0.2
1,266,435 - ychQ b1213 0.73 -0.3
1,266,483 + ychQ b1213 0.85 +0.9
1,266,522 - +0.7
1,266,644 - ychA b1214 0.12 +0.8
1,266,701 + ychA b1214 0.20 +0.0
1,266,737 + ychA b1214 0.24 +1.1
1,266,990 + ychA b1214 0.55 +0.0
1,267,094 + ychA b1214 0.68 -0.9
1,267,104 - ychA b1214 0.69 -0.2
1,267,139 + ychA b1214 0.74 -0.8
1,267,242 + ychA b1214 0.86 -0.0
1,267,242 + ychA b1214 0.86 -0.3
1,267,242 + ychA b1214 0.86 -0.0
1,267,274 + -1.2
1,267,274 + -0.5
1,267,274 - +1.8
1,267,276 + -0.5
1,267,276 + -0.3

Or see this region's nucleotide sequence