Experiment: LB Aerobic with Sulfamonomethoxine 0.000125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ybjL and ybjM are separated by 269 nucleotides ybjM and grxA are separated by 29 nucleotides grxA and ybjC are separated by 159 nucleotides ybjC and nfsA overlap by 17 nucleotides
b0847: ybjL - hypothetical protein (NCBI), at 887,357 to 889,042
ybjL
b0848: ybjM - predicted inner membrane protein (NCBI), at 889,312 to 889,689
ybjM
b0849: grxA - glutaredoxin 1, redox coenzyme for ribonucleotide reductase (RNR1a) (NCBI), at 889,719 to 889,976
grxA
b0850: ybjC - predicted inner membrane protein (NCBI), at 890,136 to 890,423
ybjC
b0851: nfsA - nitroreductase A, NADPH-dependent, FMN-dependent (NCBI), at 890,407 to 891,129
nfsA
Position (kb) 889
890 Strain fitness (log2 ratio) -2
-1
0
1
2 at 888.723 kb on - strand, within ybjL at 888.723 kb on - strand, within ybjL at 888.725 kb on + strand, within ybjL at 888.740 kb on + strand, within ybjL at 888.763 kb on - strand, within ybjL at 888.822 kb on - strand, within ybjL at 888.822 kb on - strand, within ybjL at 888.866 kb on + strand, within ybjL at 888.866 kb on + strand, within ybjL at 888.894 kb on + strand at 888.894 kb on + strand at 888.894 kb on + strand at 888.902 kb on - strand at 888.902 kb on - strand at 888.960 kb on + strand at 888.964 kb on + strand at 888.965 kb on + strand at 888.971 kb on - strand at 888.978 kb on - strand at 888.992 kb on + strand at 888.993 kb on - strand at 889.038 kb on - strand at 889.038 kb on - strand at 889.038 kb on - strand at 889.129 kb on - strand at 889.129 kb on - strand at 889.141 kb on + strand at 889.180 kb on + strand at 889.180 kb on + strand at 889.192 kb on - strand at 889.192 kb on - strand at 889.192 kb on - strand at 889.198 kb on - strand at 889.198 kb on - strand at 889.208 kb on - strand at 889.208 kb on - strand at 889.215 kb on - strand at 889.220 kb on - strand at 889.222 kb on + strand at 889.226 kb on - strand at 889.228 kb on - strand at 889.228 kb on - strand at 889.233 kb on + strand at 889.235 kb on - strand at 889.244 kb on + strand at 889.248 kb on + strand at 889.258 kb on - strand at 889.291 kb on + strand at 889.308 kb on - strand at 889.344 kb on + strand at 889.344 kb on + strand at 889.404 kb on + strand, within ybjM at 889.404 kb on + strand, within ybjM at 889.460 kb on - strand, within ybjM at 889.482 kb on + strand, within ybjM at 889.482 kb on + strand, within ybjM at 889.482 kb on + strand, within ybjM at 889.482 kb on + strand, within ybjM at 889.538 kb on - strand, within ybjM at 889.538 kb on - strand, within ybjM at 889.586 kb on + strand, within ybjM at 889.586 kb on + strand, within ybjM at 889.594 kb on + strand, within ybjM at 889.604 kb on - strand, within ybjM at 889.604 kb on - strand, within ybjM at 889.650 kb on - strand, within ybjM at 889.934 kb on + strand, within grxA at 889.936 kb on + strand, within grxA at 889.938 kb on + strand, within grxA at 889.944 kb on - strand, within grxA at 889.944 kb on - strand, within grxA at 889.988 kb on + strand at 890.213 kb on + strand, within ybjC at 890.218 kb on + strand, within ybjC at 890.232 kb on - strand, within ybjC at 890.232 kb on - strand, within ybjC at 890.326 kb on + strand, within ybjC at 890.491 kb on - strand, within nfsA at 890.714 kb on - strand, within nfsA at 890.715 kb on - strand, within nfsA at 890.783 kb on + strand, within nfsA at 890.792 kb on - strand, within nfsA at 890.958 kb on + strand, within nfsA
Per-strain Table
Position Strand Gene LocusTag Fraction LB Aerobic with Sulfamonomethoxine 0.000125 mM remove 888,723 - ybjL b0847 0.81 +0.8 888,723 - ybjL b0847 0.81 +0.3 888,725 + ybjL b0847 0.81 +0.7 888,740 + ybjL b0847 0.82 +0.2 888,763 - ybjL b0847 0.83 -0.7 888,822 - ybjL b0847 0.87 +0.3 888,822 - ybjL b0847 0.87 +1.1 888,866 + ybjL b0847 0.90 +0.9 888,866 + ybjL b0847 0.90 +0.0 888,894 + +0.3 888,894 + +0.6 888,894 + -0.1 888,902 - -0.2 888,902 - -0.6 888,960 + +0.0 888,964 + +0.3 888,965 + +0.7 888,971 - +0.2 888,978 - -0.3 888,992 + +2.1 888,993 - +0.1 889,038 - -0.5 889,038 - +0.3 889,038 - -0.7 889,129 - +0.7 889,129 - +1.6 889,141 + -1.5 889,180 + +1.3 889,180 + -0.5 889,192 - +0.3 889,192 - +0.8 889,192 - -0.2 889,198 - -0.9 889,198 - -0.1 889,208 - +0.1 889,208 - +0.3 889,215 - +1.5 889,220 - +0.5 889,222 + -0.2 889,226 - +0.8 889,228 - -1.0 889,228 - -0.9 889,233 + -1.0 889,235 - +0.0 889,244 + +0.3 889,248 + -0.2 889,258 - -0.4 889,291 + -1.2 889,308 - +0.4 889,344 + +0.1 889,344 + -0.0 889,404 + ybjM b0848 0.24 -0.1 889,404 + ybjM b0848 0.24 -0.9 889,460 - ybjM b0848 0.39 -2.0 889,482 + ybjM b0848 0.45 -0.5 889,482 + ybjM b0848 0.45 -1.3 889,482 + ybjM b0848 0.45 +1.5 889,482 + ybjM b0848 0.45 +0.5 889,538 - ybjM b0848 0.60 -2.0 889,538 - ybjM b0848 0.60 +0.3 889,586 + ybjM b0848 0.72 -0.0 889,586 + ybjM b0848 0.72 -0.1 889,594 + ybjM b0848 0.75 -0.2 889,604 - ybjM b0848 0.77 +0.0 889,604 - ybjM b0848 0.77 -0.2 889,650 - ybjM b0848 0.89 -1.2 889,934 + grxA b0849 0.83 +0.6 889,936 + grxA b0849 0.84 +0.3 889,938 + grxA b0849 0.85 -0.1 889,944 - grxA b0849 0.87 +1.7 889,944 - grxA b0849 0.87 -0.2 889,988 + -0.5 890,213 + ybjC b0850 0.27 -0.5 890,218 + ybjC b0850 0.28 +1.3 890,232 - ybjC b0850 0.33 +0.0 890,232 - ybjC b0850 0.33 -1.0 890,326 + ybjC b0850 0.66 +0.7 890,491 - nfsA b0851 0.12 +0.9 890,714 - nfsA b0851 0.42 -0.1 890,715 - nfsA b0851 0.43 -1.3 890,783 + nfsA b0851 0.52 +0.2 890,792 - nfsA b0851 0.53 +1.1 890,958 + nfsA b0851 0.76 +0.6
Or see this region's nucleotide sequence