Experiment: LB Aerobic with Sulfamonomethoxine 0.000125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt dcuC and crcA are separated by 588 nucleotides crcA and cspE are separated by 174 nucleotides cspE and crcB are separated by 53 nucleotides crcB and ybeM are separated by 92 nucleotides
b0621: dcuC - anaerobic C4-dicarboxylate transport (NCBI), at 653,806 to 655,191
dcuC
b0622: crcA - palmitoyl transferase for Lipid A (NCBI), at 655,780 to 656,340
crcA
b0623: cspE - cold shock protein E (NCBI), at 656,515 to 656,724
cspE
b0624: crcB - camphor resistance protein CrcB (NCBI), at 656,778 to 657,161
crcB
b4581: ybeM - no description, at 657,254 to 658,041
ybeM
Position (kb) 655
656
657 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 654.850 kb on + strand, within dcuC at 654.850 kb on + strand, within dcuC at 654.896 kb on - strand, within dcuC at 654.896 kb on - strand, within dcuC at 654.897 kb on - strand, within dcuC at 654.936 kb on + strand, within dcuC at 655.012 kb on + strand, within dcuC at 655.027 kb on + strand, within dcuC at 655.030 kb on - strand, within dcuC at 655.030 kb on - strand, within dcuC at 655.030 kb on - strand, within dcuC at 655.064 kb on - strand at 655.067 kb on + strand at 655.077 kb on - strand at 655.095 kb on + strand at 655.095 kb on + strand at 655.114 kb on - strand at 655.122 kb on - strand at 655.123 kb on - strand at 655.127 kb on + strand at 655.138 kb on + strand at 655.138 kb on + strand at 655.145 kb on + strand at 655.147 kb on + strand at 655.147 kb on + strand at 655.147 kb on + strand at 655.151 kb on - strand at 655.152 kb on - strand at 655.155 kb on + strand at 655.155 kb on + strand at 655.155 kb on - strand at 655.155 kb on - strand at 655.166 kb on + strand at 655.169 kb on - strand at 655.204 kb on + strand at 655.329 kb on + strand at 655.370 kb on - strand at 655.436 kb on - strand at 655.472 kb on + strand at 655.624 kb on + strand at 655.716 kb on - strand at 655.727 kb on - strand at 655.861 kb on + strand, within crcA at 655.861 kb on + strand, within crcA at 655.866 kb on + strand, within crcA at 655.866 kb on + strand, within crcA at 655.869 kb on - strand, within crcA at 655.874 kb on - strand, within crcA at 655.907 kb on - strand, within crcA at 655.919 kb on - strand, within crcA at 655.919 kb on - strand, within crcA at 655.937 kb on - strand, within crcA at 655.937 kb on - strand, within crcA at 655.946 kb on + strand, within crcA at 655.947 kb on - strand, within crcA at 655.947 kb on - strand, within crcA at 655.949 kb on + strand, within crcA at 655.957 kb on - strand, within crcA at 655.961 kb on - strand, within crcA at 655.996 kb on - strand, within crcA at 655.998 kb on - strand, within crcA at 655.998 kb on - strand, within crcA at 655.999 kb on - strand, within crcA at 655.999 kb on - strand, within crcA at 656.001 kb on + strand, within crcA at 656.009 kb on + strand, within crcA at 656.009 kb on - strand, within crcA at 656.009 kb on - strand, within crcA at 656.009 kb on - strand, within crcA at 656.058 kb on + strand, within crcA at 656.118 kb on - strand, within crcA at 656.118 kb on - strand, within crcA at 656.129 kb on - strand, within crcA at 656.191 kb on - strand, within crcA at 656.284 kb on - strand, within crcA at 656.298 kb on + strand at 656.428 kb on + strand at 656.535 kb on + strand at 656.553 kb on + strand, within cspE at 656.648 kb on + strand, within cspE at 656.813 kb on + strand at 656.927 kb on + strand, within crcB at 656.935 kb on - strand, within crcB at 656.992 kb on + strand, within crcB at 657.065 kb on - strand, within crcB at 657.121 kb on + strand, within crcB at 657.208 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB Aerobic with Sulfamonomethoxine 0.000125 mM remove 654,850 + dcuC b0621 0.75 +0.6 654,850 + dcuC b0621 0.75 +0.2 654,896 - dcuC b0621 0.79 -0.2 654,896 - dcuC b0621 0.79 +0.4 654,897 - dcuC b0621 0.79 +0.3 654,936 + dcuC b0621 0.82 -0.2 655,012 + dcuC b0621 0.87 +1.4 655,027 + dcuC b0621 0.88 +0.2 655,030 - dcuC b0621 0.88 +1.7 655,030 - dcuC b0621 0.88 +0.3 655,030 - dcuC b0621 0.88 -0.4 655,064 - +1.2 655,067 + -1.2 655,077 - +1.4 655,095 + +0.1 655,095 + -1.4 655,114 - -0.3 655,122 - +1.4 655,123 - +1.4 655,127 + +0.0 655,138 + +0.7 655,138 + -1.6 655,145 + +2.0 655,147 + -1.2 655,147 + +3.0 655,147 + -0.2 655,151 - -0.9 655,152 - -0.6 655,155 + +0.8 655,155 + +0.4 655,155 - +1.4 655,155 - +1.4 655,166 + -0.6 655,169 - +1.4 655,204 + +0.4 655,329 + +1.4 655,370 - +1.4 655,436 - +1.4 655,472 + +1.4 655,624 + +1.4 655,716 - +1.4 655,727 - +1.4 655,861 + crcA b0622 0.14 +1.4 655,861 + crcA b0622 0.14 +1.4 655,866 + crcA b0622 0.15 +1.4 655,866 + crcA b0622 0.15 +1.4 655,869 - crcA b0622 0.16 +1.4 655,874 - crcA b0622 0.17 +1.4 655,907 - crcA b0622 0.23 -0.6 655,919 - crcA b0622 0.25 -0.4 655,919 - crcA b0622 0.25 +0.4 655,937 - crcA b0622 0.28 -1.6 655,937 - crcA b0622 0.28 -0.6 655,946 + crcA b0622 0.30 -0.9 655,947 - crcA b0622 0.30 -0.2 655,947 - crcA b0622 0.30 -0.2 655,949 + crcA b0622 0.30 +0.2 655,957 - crcA b0622 0.32 +0.7 655,961 - crcA b0622 0.32 +0.6 655,996 - crcA b0622 0.39 +0.0 655,998 - crcA b0622 0.39 -0.2 655,998 - crcA b0622 0.39 +0.3 655,999 - crcA b0622 0.39 -0.9 655,999 - crcA b0622 0.39 -2.7 656,001 + crcA b0622 0.39 -0.6 656,009 + crcA b0622 0.41 +0.2 656,009 - crcA b0622 0.41 -0.9 656,009 - crcA b0622 0.41 -0.2 656,009 - crcA b0622 0.41 -0.6 656,058 + crcA b0622 0.50 -0.5 656,118 - crcA b0622 0.60 -0.9 656,118 - crcA b0622 0.60 -0.2 656,129 - crcA b0622 0.62 -0.9 656,191 - crcA b0622 0.73 -0.2 656,284 - crcA b0622 0.90 -0.5 656,298 + -0.3 656,428 + +0.1 656,535 + -0.4 656,553 + cspE b0623 0.18 +0.4 656,648 + cspE b0623 0.63 -0.1 656,813 + -0.9 656,927 + crcB b0624 0.39 -0.1 656,935 - crcB b0624 0.41 -1.5 656,992 + crcB b0624 0.56 -0.3 657,065 - crcB b0624 0.75 -0.0 657,121 + crcB b0624 0.89 +0.9 657,208 + +0.0
Or see this region's nucleotide sequence