Strain Fitness in Escherichia coli BW25113 around b2427

Experiment: LB Aerobic with Sulfamonomethoxine 0.00025 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntucpA and yfeT are separated by 128 nucleotidesyfeT and yfeU are separated by 163 nucleotides b2426: ucpA - putative oxidoreductase (VIMSS), at 2,541,854 to 2,542,645 ucpA b2427: yfeT - predicted DNA-binding transcriptional regulator (NCBI), at 2,542,774 to 2,543,631 yfeT b2428: yfeU - N-acetylmuramic acid-6-phosphate etherase (NCBI), at 2,543,795 to 2,544,691 yfeU Position (kb) 2542 2543 2544Strain fitness (log2 ratio) -2 -1 0 1at 2542.113 kb on + strand, within ucpAat 2542.215 kb on - strand, within ucpAat 2542.432 kb on - strand, within ucpAat 2542.661 kb on + strandat 2542.661 kb on + strandat 2542.748 kb on + strandat 2542.796 kb on - strandat 2542.833 kb on - strandat 2542.833 kb on - strandat 2543.173 kb on - strand, within yfeTat 2543.193 kb on - strand, within yfeTat 2543.230 kb on - strand, within yfeTat 2543.231 kb on + strand, within yfeTat 2543.231 kb on + strand, within yfeTat 2543.365 kb on + strand, within yfeTat 2543.365 kb on + strand, within yfeTat 2543.376 kb on + strand, within yfeTat 2543.410 kb on + strand, within yfeTat 2543.428 kb on - strand, within yfeTat 2543.648 kb on - strandat 2543.683 kb on + strandat 2543.880 kb on - strandat 2543.935 kb on - strand, within yfeUat 2544.000 kb on + strand, within yfeUat 2544.058 kb on - strand, within yfeUat 2544.130 kb on - strand, within yfeUat 2544.130 kb on - strand, within yfeUat 2544.390 kb on - strand, within yfeUat 2544.425 kb on + strand, within yfeUat 2544.425 kb on + strand, within yfeU

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Aerobic with Sulfamonomethoxine 0.00025 mM
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2,542,113 + ucpA b2426 0.33 -0.6
2,542,215 - ucpA b2426 0.46 +1.0
2,542,432 - ucpA b2426 0.73 +0.7
2,542,661 + -2.3
2,542,661 + -0.9
2,542,748 + +0.1
2,542,796 - +0.1
2,542,833 - +0.7
2,542,833 - -1.0
2,543,173 - yfeT b2427 0.47 +1.0
2,543,193 - yfeT b2427 0.49 +0.5
2,543,230 - yfeT b2427 0.53 -0.3
2,543,231 + yfeT b2427 0.53 -0.2
2,543,231 + yfeT b2427 0.53 +0.3
2,543,365 + yfeT b2427 0.69 +0.7
2,543,365 + yfeT b2427 0.69 +1.4
2,543,376 + yfeT b2427 0.70 +0.2
2,543,410 + yfeT b2427 0.74 +0.2
2,543,428 - yfeT b2427 0.76 +0.0
2,543,648 - +0.7
2,543,683 + -0.8
2,543,880 - -0.3
2,543,935 - yfeU b2428 0.16 -0.5
2,544,000 + yfeU b2428 0.23 -1.0
2,544,058 - yfeU b2428 0.29 -0.2
2,544,130 - yfeU b2428 0.37 +0.1
2,544,130 - yfeU b2428 0.37 +0.6
2,544,390 - yfeU b2428 0.66 -0.0
2,544,425 + yfeU b2428 0.70 +1.4
2,544,425 + yfeU b2428 0.70 +0.5

Or see this region's nucleotide sequence