Experiment: LB Aerobic with Sulfamonomethoxine 0.00025 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt btuD and btuE overlap by 1 nucleotides btuE and btuC are separated by 62 nucleotides btuC and ihfA are separated by 100 nucleotides ihfA and pheT are separated by 4 nucleotides
b1709: btuD - vitamin B12-transporter ATPase (NCBI), at 1,790,833 to 1,791,582
btuD
b1710: btuE - predicted glutathione peroxidase (NCBI), at 1,791,582 to 1,792,133
btuE
b1711: btuC - vtamin B12-transporter permease (NCBI), at 1,792,196 to 1,793,176
btuC
b1712: ihfA - integration host factor subunit alpha (NCBI), at 1,793,277 to 1,793,576
ihfA
b1713: pheT - phenylalanyl-tRNA synthetase beta subunit (NCBI), at 1,793,581 to 1,795,968
pheT
Position (kb)
1792
1793
1794 Strain fitness (log2 ratio)
-1
0
1 at 1791.494 kb on - strand, within btuD at 1791.821 kb on - strand, within btuE at 1791.821 kb on - strand, within btuE at 1791.931 kb on - strand, within btuE at 1792.288 kb on - strand at 1792.295 kb on + strand, within btuC at 1792.295 kb on + strand, within btuC at 1792.423 kb on - strand, within btuC at 1792.432 kb on + strand, within btuC at 1792.492 kb on + strand, within btuC at 1792.566 kb on + strand, within btuC at 1792.592 kb on - strand, within btuC at 1792.783 kb on + strand, within btuC at 1792.820 kb on - strand, within btuC at 1792.846 kb on + strand, within btuC at 1792.980 kb on + strand, within btuC at 1793.064 kb on + strand, within btuC at 1793.108 kb on + strand at 1793.167 kb on + strand at 1793.280 kb on - strand at 1793.330 kb on + strand, within ihfA at 1793.353 kb on + strand, within ihfA at 1793.417 kb on + strand, within ihfA at 1793.533 kb on - strand, within ihfA
Per-strain Table
Position Strand Gene LocusTag Fraction LB Aerobic with Sulfamonomethoxine 0.00025 mM remove 1,791,494 - btuD b1709 0.88 +0.1 1,791,821 - btuE b1710 0.43 +0.4 1,791,821 - btuE b1710 0.43 -0.6 1,791,931 - btuE b1710 0.63 -0.5 1,792,288 - -0.6 1,792,295 + btuC b1711 0.10 -1.3 1,792,295 + btuC b1711 0.10 +0.5 1,792,423 - btuC b1711 0.23 -1.6 1,792,432 + btuC b1711 0.24 -1.7 1,792,492 + btuC b1711 0.30 -1.0 1,792,566 + btuC b1711 0.38 -1.3 1,792,592 - btuC b1711 0.40 -1.7 1,792,783 + btuC b1711 0.60 -1.5 1,792,820 - btuC b1711 0.64 -1.3 1,792,846 + btuC b1711 0.66 -0.9 1,792,980 + btuC b1711 0.80 -1.0 1,793,064 + btuC b1711 0.88 -0.0 1,793,108 + -0.1 1,793,167 + +0.9 1,793,280 - -0.4 1,793,330 + ihfA b1712 0.18 +1.5 1,793,353 + ihfA b1712 0.25 +1.5 1,793,417 + ihfA b1712 0.47 +1.5 1,793,533 - ihfA b1712 0.85 +1.7
Or see this region's nucleotide sequence