Strain Fitness in Escherichia coli BW25113 around b1618

Experiment: LB Aerobic with Oxytetracycline 0.0000003125 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntuidA and uidR are separated by 390 nucleotidesuidR and hdhA are separated by 220 nucleotides b1617: uidA - beta-D-glucuronidase (NCBI), at 1,692,284 to 1,694,095 uidA b1618: uidR - DNA-binding transcriptional repressor (NCBI), at 1,694,486 to 1,695,076 uidR b1619: hdhA - 7-alpha-hydroxysteroid dehydrogenase (NCBI), at 1,695,297 to 1,696,064 hdhA Position (kb) 1694 1695 1696Strain fitness (log2 ratio) -1 0 1 2at 1693.548 kb on - strand, within uidAat 1693.548 kb on - strand, within uidAat 1693.571 kb on - strand, within uidAat 1693.571 kb on - strand, within uidAat 1693.836 kb on - strand, within uidAat 1693.954 kb on + strandat 1694.042 kb on - strandat 1694.100 kb on - strandat 1694.177 kb on - strandat 1694.267 kb on - strandat 1694.629 kb on + strandat 1694.629 kb on + strand, within uidRat 1694.746 kb on - strand, within uidRat 1694.873 kb on + strand, within uidRat 1694.873 kb on + strand, within uidRat 1694.938 kb on + strand, within uidRat 1695.083 kb on + strandat 1695.089 kb on - strandat 1695.234 kb on - strandat 1695.316 kb on - strandat 1695.368 kb on + strandat 1695.368 kb on + strandat 1695.393 kb on - strand, within hdhAat 1695.425 kb on + strand, within hdhAat 1695.432 kb on - strand, within hdhAat 1695.432 kb on - strand, within hdhAat 1695.558 kb on - strand, within hdhAat 1695.574 kb on + strand, within hdhAat 1695.574 kb on + strand, within hdhAat 1695.574 kb on + strand, within hdhAat 1695.582 kb on - strand, within hdhAat 1695.582 kb on - strand, within hdhAat 1695.592 kb on - strand, within hdhAat 1695.592 kb on - strand, within hdhAat 1695.690 kb on - strand, within hdhAat 1695.690 kb on - strand, within hdhAat 1695.724 kb on - strand, within hdhAat 1695.758 kb on - strand, within hdhAat 1695.771 kb on - strand, within hdhAat 1695.771 kb on - strand, within hdhAat 1695.779 kb on - strand, within hdhAat 1695.779 kb on - strand, within hdhAat 1695.841 kb on - strand, within hdhAat 1695.841 kb on - strand, within hdhAat 1695.901 kb on - strand, within hdhAat 1695.914 kb on - strand, within hdhAat 1695.925 kb on - strand, within hdhAat 1695.959 kb on + strand, within hdhAat 1695.959 kb on + strand, within hdhAat 1696.001 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Aerobic with Oxytetracycline 0.0000003125 mM
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1,693,548 - uidA b1617 0.70 +0.0
1,693,548 - uidA b1617 0.70 +1.0
1,693,571 - uidA b1617 0.71 -0.3
1,693,571 - uidA b1617 0.71 -0.9
1,693,836 - uidA b1617 0.86 +0.7
1,693,954 + +0.2
1,694,042 - +0.9
1,694,100 - +0.9
1,694,177 - +0.2
1,694,267 - +0.5
1,694,629 + +0.0
1,694,629 + uidR b1618 0.24 -1.2
1,694,746 - uidR b1618 0.44 +0.1
1,694,873 + uidR b1618 0.65 +0.8
1,694,873 + uidR b1618 0.65 -0.1
1,694,938 + uidR b1618 0.76 +2.0
1,695,083 + +0.5
1,695,089 - +1.2
1,695,234 - +1.1
1,695,316 - +0.3
1,695,368 + -0.9
1,695,368 + +2.1
1,695,393 - hdhA b1619 0.12 -0.2
1,695,425 + hdhA b1619 0.17 +0.6
1,695,432 - hdhA b1619 0.18 -0.4
1,695,432 - hdhA b1619 0.18 -0.0
1,695,558 - hdhA b1619 0.34 -0.5
1,695,574 + hdhA b1619 0.36 +1.3
1,695,574 + hdhA b1619 0.36 -0.2
1,695,574 + hdhA b1619 0.36 +0.3
1,695,582 - hdhA b1619 0.37 +1.1
1,695,582 - hdhA b1619 0.37 +0.1
1,695,592 - hdhA b1619 0.38 -0.7
1,695,592 - hdhA b1619 0.38 +1.3
1,695,690 - hdhA b1619 0.51 -0.2
1,695,690 - hdhA b1619 0.51 +0.2
1,695,724 - hdhA b1619 0.56 +2.7
1,695,758 - hdhA b1619 0.60 -0.1
1,695,771 - hdhA b1619 0.62 +0.1
1,695,771 - hdhA b1619 0.62 +0.1
1,695,779 - hdhA b1619 0.63 -0.5
1,695,779 - hdhA b1619 0.63 +0.0
1,695,841 - hdhA b1619 0.71 -1.2
1,695,841 - hdhA b1619 0.71 -0.0
1,695,901 - hdhA b1619 0.79 +0.3
1,695,914 - hdhA b1619 0.80 -0.1
1,695,925 - hdhA b1619 0.82 +1.3
1,695,959 + hdhA b1619 0.86 -0.7
1,695,959 + hdhA b1619 0.86 -0.2
1,696,001 - +0.9

Or see this region's nucleotide sequence