Experiment: LB Aerobic with Oxytetracycline 0.0000003125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt gapC and cybB are separated by 188 nucleotides cybB and rydC are separated by 10 nucleotides rydC and ydcA are separated by 170 nucleotides ydcA and hokB are separated by 71 nucleotides hokB and mokB overlap by 110 nucleotides mokB and sokB overlap by 11 nucleotides sokB and trg are separated by 295 nucleotides
b4493: gapC - no description, at 1,487,737 to 1,488,737
gapC
b1418: cybB - cytochrome b(561) (VIMSS), at 1,488,926 to 1,489,456
cybB
b4597: rydC - sRNA regulator of yejABEF (RefSeq), at 1,489,467 to 1,489,530
rydC
b1419: ydcA - hypothetical protein (NCBI), at 1,489,701 to 1,489,874
ydcA
b4428: hokB - toxic polypeptide, small (NCBI), at 1,489,946 to 1,490,095
hokB
b1420: mokB - regulatory peptide (NCBI), at 1,489,986 to 1,490,153
mokB
b4429: sokB - Antisense sRNA blocking mokB, and hence hokB, translation (RefSeq), at 1,490,143 to 1,490,198
sokB
b1421: trg - methyl-accepting chemotaxis protein III, ribose and galactose sensor receptor (NCBI), at 1,490,494 to 1,492,134
trg
Position (kb)
1489
1490 Strain fitness (log2 ratio)
-1
0
1 at 1488.827 kb on + strand at 1489.026 kb on + strand, within cybB at 1489.028 kb on + strand, within cybB at 1489.032 kb on + strand, within cybB at 1489.032 kb on + strand, within cybB at 1489.616 kb on + strand at 1489.627 kb on + strand at 1489.651 kb on + strand at 1489.659 kb on - strand at 1489.659 kb on - strand at 1489.794 kb on + strand, within ydcA at 1489.794 kb on + strand, within ydcA at 1489.802 kb on - strand, within ydcA at 1489.802 kb on - strand, within ydcA at 1489.804 kb on + strand, within ydcA at 1489.804 kb on + strand, within ydcA at 1490.029 kb on + strand at 1490.063 kb on + strand at 1490.099 kb on - strand, within mokB at 1490.344 kb on - strand at 1490.506 kb on + strand at 1490.543 kb on - strand at 1490.641 kb on - strand at 1490.678 kb on + strand, within trg at 1490.720 kb on + strand, within trg at 1490.724 kb on + strand, within trg at 1490.838 kb on - strand, within trg
Per-strain Table
Position Strand Gene LocusTag Fraction LB Aerobic with Oxytetracycline 0.0000003125 mM remove 1,488,827 + -0.1 1,489,026 + cybB b1418 0.19 +0.8 1,489,028 + cybB b1418 0.19 +0.2 1,489,032 + cybB b1418 0.20 -1.0 1,489,032 + cybB b1418 0.20 +0.2 1,489,616 + -0.2 1,489,627 + +0.7 1,489,651 + +0.7 1,489,659 - -0.6 1,489,659 - -0.5 1,489,794 + ydcA b1419 0.53 -0.4 1,489,794 + ydcA b1419 0.53 +0.6 1,489,802 - ydcA b1419 0.58 +0.4 1,489,802 - ydcA b1419 0.58 +1.1 1,489,804 + ydcA b1419 0.59 +0.4 1,489,804 + ydcA b1419 0.59 +0.5 1,490,029 + -0.1 1,490,063 + +1.1 1,490,099 - mokB b1420 0.67 -0.6 1,490,344 - +0.4 1,490,506 + -1.4 1,490,543 - +0.4 1,490,641 - +0.4 1,490,678 + trg b1421 0.11 +0.0 1,490,720 + trg b1421 0.14 +1.1 1,490,724 + trg b1421 0.14 -0.8 1,490,838 - trg b1421 0.21 +0.2
Or see this region's nucleotide sequence