Strain Fitness in Escherichia coli BW25113 around b1120

Experiment: LB Aerobic with Spiramycin 0.0000625 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntnagK and cobB are separated by 126 nucleotidescobB and ycfZ are separated by 119 nucleotidesycfZ and ymfA overlap by 4 nucleotides b1119: nagK - N-acetyl-D-glucosamine kinase (NCBI), at 1,177,816 to 1,178,727 nagK b1120: cobB - deacetylase of acs and cheY, regulates chemotaxis (NCBI), at 1,178,854 to 1,179,582 cobB b1121: ycfZ - predicted inner membrane protein (NCBI), at 1,179,702 to 1,180,490 ycfZ b1122: ymfA - orf, hypothetical protein (VIMSS), at 1,180,487 to 1,180,948 ymfA Position (kb) 1178 1179 1180Strain fitness (log2 ratio) -2 -1 0 1at 1177.988 kb on - strand, within nagKat 1177.988 kb on - strand, within nagKat 1178.081 kb on + strand, within nagKat 1178.086 kb on + strand, within nagKat 1178.100 kb on + strand, within nagKat 1178.411 kb on - strand, within nagKat 1178.423 kb on + strand, within nagKat 1178.423 kb on + strand, within nagKat 1178.483 kb on + strand, within nagKat 1178.486 kb on - strand, within nagKat 1178.567 kb on - strand, within nagKat 1178.692 kb on - strandat 1178.693 kb on + strandat 1178.708 kb on + strandat 1178.708 kb on + strandat 1178.766 kb on + strandat 1178.766 kb on + strandat 1178.875 kb on + strandat 1178.875 kb on + strandat 1178.880 kb on - strandat 1178.880 kb on - strandat 1179.036 kb on - strand, within cobBat 1179.142 kb on + strand, within cobBat 1179.161 kb on + strand, within cobBat 1179.161 kb on + strand, within cobBat 1179.195 kb on - strand, within cobBat 1179.319 kb on + strand, within cobBat 1179.396 kb on + strand, within cobBat 1179.413 kb on + strand, within cobBat 1179.413 kb on + strand, within cobBat 1179.421 kb on - strand, within cobBat 1179.526 kb on + strandat 1179.552 kb on - strandat 1179.584 kb on + strandat 1179.584 kb on + strandat 1179.584 kb on + strandat 1179.594 kb on + strandat 1179.758 kb on - strandat 1179.768 kb on - strandat 1179.785 kb on + strand, within ycfZat 1179.833 kb on + strand, within ycfZat 1179.837 kb on - strand, within ycfZat 1179.922 kb on + strand, within ycfZat 1179.922 kb on + strand, within ycfZat 1179.922 kb on + strand, within ycfZat 1179.922 kb on + strand, within ycfZat 1179.922 kb on + strand, within ycfZat 1179.943 kb on + strand, within ycfZat 1179.992 kb on + strand, within ycfZat 1180.234 kb on - strand, within ycfZat 1180.389 kb on - strand, within ycfZat 1180.389 kb on - strand, within ycfZat 1180.438 kb on + strandat 1180.457 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Aerobic with Spiramycin 0.0000625 mM
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1,177,988 - nagK b1119 0.19 +0.1
1,177,988 - nagK b1119 0.19 -1.9
1,178,081 + nagK b1119 0.29 -0.3
1,178,086 + nagK b1119 0.30 +0.1
1,178,100 + nagK b1119 0.31 +0.7
1,178,411 - nagK b1119 0.65 -0.0
1,178,423 + nagK b1119 0.67 -0.5
1,178,423 + nagK b1119 0.67 -0.6
1,178,483 + nagK b1119 0.73 +0.1
1,178,486 - nagK b1119 0.73 -0.8
1,178,567 - nagK b1119 0.82 -0.5
1,178,692 - +0.1
1,178,693 + +0.5
1,178,708 + -0.1
1,178,708 + -0.9
1,178,766 + -0.5
1,178,766 + +1.0
1,178,875 + -0.2
1,178,875 + -1.1
1,178,880 - +0.4
1,178,880 - +0.4
1,179,036 - cobB b1120 0.25 +0.9
1,179,142 + cobB b1120 0.40 +1.1
1,179,161 + cobB b1120 0.42 +1.6
1,179,161 + cobB b1120 0.42 -0.5
1,179,195 - cobB b1120 0.47 -0.6
1,179,319 + cobB b1120 0.64 +1.7
1,179,396 + cobB b1120 0.74 +1.1
1,179,413 + cobB b1120 0.77 +1.7
1,179,413 + cobB b1120 0.77 +0.7
1,179,421 - cobB b1120 0.78 -0.9
1,179,526 + -1.2
1,179,552 - +1.4
1,179,584 + -0.1
1,179,584 + +0.7
1,179,584 + +0.1
1,179,594 + +0.2
1,179,758 - +1.4
1,179,768 - +1.4
1,179,785 + ycfZ b1121 0.11 -2.1
1,179,833 + ycfZ b1121 0.17 -0.2
1,179,837 - ycfZ b1121 0.17 +1.4
1,179,922 + ycfZ b1121 0.28 +1.4
1,179,922 + ycfZ b1121 0.28 +1.4
1,179,922 + ycfZ b1121 0.28 +1.4
1,179,922 + ycfZ b1121 0.28 +1.4
1,179,922 + ycfZ b1121 0.28 +1.4
1,179,943 + ycfZ b1121 0.31 +1.4
1,179,992 + ycfZ b1121 0.37 +1.4
1,180,234 - ycfZ b1121 0.67 +1.4
1,180,389 - ycfZ b1121 0.87 +1.4
1,180,389 - ycfZ b1121 0.87 +1.4
1,180,438 + +1.4
1,180,457 + +1.4

Or see this region's nucleotide sequence