Experiment: LB Aerobic with Spiramycin 0.000125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cynR and cynT are separated by 108 nucleotides cynT and cynS are separated by 30 nucleotides cynS and cynX are separated by 32 nucleotides
b0338: cynR - DNA-binding transcriptional dual regulator (NCBI), at 357,015 to 357,914
cynR
b0339: cynT - carbonic anhydrase (NCBI), at 358,023 to 358,682
cynT
b0340: cynS - cyanate hydratase (NCBI), at 358,713 to 359,183
cynS
b0341: cynX - predicted cyanate transporter (NCBI), at 359,216 to 360,370
cynX
Position (kb) 358
359
360 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 357.720 kb on + strand, within cynR at 357.787 kb on + strand, within cynR at 357.827 kb on + strand at 357.849 kb on + strand at 357.849 kb on + strand at 357.880 kb on - strand at 357.880 kb on - strand at 357.910 kb on - strand at 358.023 kb on - strand at 358.077 kb on + strand at 358.087 kb on + strand at 358.103 kb on - strand, within cynT at 358.206 kb on - strand, within cynT at 358.206 kb on - strand, within cynT at 358.350 kb on + strand, within cynT at 358.386 kb on - strand, within cynT at 358.548 kb on + strand, within cynT at 358.562 kb on + strand, within cynT at 358.630 kb on + strand at 358.757 kb on - strand at 358.912 kb on - strand, within cynS at 358.912 kb on - strand, within cynS at 359.021 kb on + strand, within cynS at 359.124 kb on - strand, within cynS at 359.279 kb on + strand at 359.279 kb on + strand at 359.393 kb on - strand, within cynX at 359.491 kb on - strand, within cynX at 359.593 kb on + strand, within cynX at 359.593 kb on + strand, within cynX at 359.604 kb on - strand, within cynX at 359.606 kb on + strand, within cynX at 359.696 kb on - strand, within cynX at 359.785 kb on - strand, within cynX at 359.792 kb on + strand, within cynX at 359.839 kb on - strand, within cynX at 359.839 kb on - strand, within cynX at 359.916 kb on + strand, within cynX at 359.916 kb on + strand, within cynX at 359.943 kb on + strand, within cynX at 360.002 kb on + strand at 360.002 kb on + strand, within cynX at 360.010 kb on - strand, within cynX at 360.096 kb on + strand, within cynX at 360.096 kb on + strand, within cynX at 360.104 kb on - strand, within cynX at 360.109 kb on - strand, within cynX
Per-strain Table
Position Strand Gene LocusTag Fraction LB Aerobic with Spiramycin 0.000125 mM remove 357,720 + cynR b0338 0.78 -0.2 357,787 + cynR b0338 0.86 +0.1 357,827 + -0.4 357,849 + -0.6 357,849 + -0.9 357,880 - -0.5 357,880 - +0.4 357,910 - +0.2 358,023 - +0.2 358,077 + -2.8 358,087 + -0.6 358,103 - cynT b0339 0.12 -1.5 358,206 - cynT b0339 0.28 -1.8 358,206 - cynT b0339 0.28 -0.9 358,350 + cynT b0339 0.50 +1.2 358,386 - cynT b0339 0.55 -0.1 358,548 + cynT b0339 0.80 +0.3 358,562 + cynT b0339 0.82 -0.4 358,630 + -0.1 358,757 - +0.1 358,912 - cynS b0340 0.42 -0.3 358,912 - cynS b0340 0.42 -0.7 359,021 + cynS b0340 0.65 -0.2 359,124 - cynS b0340 0.87 +0.6 359,279 + +1.4 359,279 + -2.6 359,393 - cynX b0341 0.15 +0.8 359,491 - cynX b0341 0.24 -0.1 359,593 + cynX b0341 0.33 +0.8 359,593 + cynX b0341 0.33 -0.1 359,604 - cynX b0341 0.34 -0.4 359,606 + cynX b0341 0.34 -1.2 359,696 - cynX b0341 0.42 +0.4 359,785 - cynX b0341 0.49 +0.1 359,792 + cynX b0341 0.50 +0.4 359,839 - cynX b0341 0.54 -0.3 359,839 - cynX b0341 0.54 +0.8 359,916 + cynX b0341 0.61 -0.8 359,916 + cynX b0341 0.61 +0.1 359,943 + cynX b0341 0.63 +0.4 360,002 + +0.8 360,002 + cynX b0341 0.68 +1.8 360,010 - cynX b0341 0.69 +0.4 360,096 + cynX b0341 0.76 +0.3 360,096 + cynX b0341 0.76 +0.1 360,104 - cynX b0341 0.77 +0.3 360,109 - cynX b0341 0.77 -1.1
Or see this region's nucleotide sequence