Strain Fitness in Escherichia coli BW25113 around b0089

Experiment: LB Aerobic with Spiramycin 0.000125 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntmurD and ftsW overlap by 1 nucleotidesftsW and murG overlap by 4 nucleotides b0088: murD - UDP-N-acetylmuramoyl-L-alanyl-D-glutamatesynthetase (NCBI), at 97,087 to 98,403 murD b0089: ftsW - integral membrane protein involved in stabilizing FstZ ring during cell division (NCBI), at 98,403 to 99,647 ftsW b0090: murG - N-acetylglucosaminyl transferase (NCBI), at 99,644 to 100,711 murG Position (kb) 98 99 100Strain fitness (log2 ratio) -2 -1 0 1at 97.787 kb on + strand, within murDat 98.441 kb on + strandat 98.490 kb on + strandat 98.502 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Aerobic with Spiramycin 0.000125 mM
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97,787 + murD b0088 0.53 +0.7
98,441 + -0.2
98,490 + -1.9
98,502 + -0.0

Or see this region's nucleotide sequence