Experiment: LB Aerobic with Phenazine ethosulfate 0.0000625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pldB and yigL are separated by 7 nucleotides yigL and yigM are separated by 75 nucleotides yigM and metR overlap by 113 nucleotides
b3825: pldB - lysophospholipase L(2) (NCBI), at 4,007,193 to 4,008,215
pldB
b3826: yigL - predicted hydrolase (NCBI), at 4,008,223 to 4,009,023
yigL
b3827: yigM - predicted inner membrane protein (NCBI), at 4,009,099 to 4,009,998
yigM
b3828: metR - DNA-binding transcriptional activator, homocysteine-binding (NCBI), at 4,009,886 to 4,010,839
metR
Position (kb)
4008
4009
4010 Strain fitness (log2 ratio)
-2
-1
0
1 at 4007.264 kb on - strand at 4007.264 kb on - strand at 4007.289 kb on - strand at 4007.289 kb on - strand at 4007.319 kb on - strand, within pldB at 4007.345 kb on + strand, within pldB at 4007.345 kb on + strand, within pldB at 4007.354 kb on - strand, within pldB at 4007.357 kb on + strand, within pldB at 4007.357 kb on + strand, within pldB at 4007.361 kb on + strand, within pldB at 4007.364 kb on - strand, within pldB at 4007.366 kb on + strand, within pldB at 4007.379 kb on - strand, within pldB at 4007.380 kb on + strand, within pldB at 4007.382 kb on + strand, within pldB at 4007.411 kb on + strand, within pldB at 4007.421 kb on - strand, within pldB at 4007.421 kb on - strand, within pldB at 4007.483 kb on + strand, within pldB at 4007.483 kb on + strand, within pldB at 4007.491 kb on - strand, within pldB at 4007.518 kb on - strand, within pldB at 4007.526 kb on - strand, within pldB at 4007.559 kb on + strand, within pldB at 4007.559 kb on + strand, within pldB at 4007.564 kb on + strand, within pldB at 4007.565 kb on + strand, within pldB at 4007.590 kb on + strand, within pldB at 4007.614 kb on - strand, within pldB at 4007.652 kb on + strand, within pldB at 4007.667 kb on + strand, within pldB at 4007.667 kb on + strand, within pldB at 4007.731 kb on + strand, within pldB at 4007.731 kb on + strand, within pldB at 4007.749 kb on - strand, within pldB at 4007.749 kb on - strand, within pldB at 4007.811 kb on + strand, within pldB at 4007.811 kb on + strand, within pldB at 4007.861 kb on + strand, within pldB at 4007.869 kb on - strand, within pldB at 4007.869 kb on - strand, within pldB at 4007.871 kb on - strand, within pldB at 4007.875 kb on + strand, within pldB at 4007.875 kb on + strand, within pldB at 4007.881 kb on - strand, within pldB at 4007.903 kb on - strand, within pldB at 4008.002 kb on - strand, within pldB at 4008.002 kb on - strand, within pldB at 4008.017 kb on + strand, within pldB at 4008.017 kb on - strand, within pldB at 4008.025 kb on - strand, within pldB at 4008.041 kb on + strand, within pldB at 4008.054 kb on - strand, within pldB at 4008.060 kb on - strand, within pldB at 4008.066 kb on + strand, within pldB at 4008.086 kb on + strand, within pldB at 4008.086 kb on + strand, within pldB at 4008.225 kb on + strand at 4008.230 kb on + strand at 4008.230 kb on + strand at 4008.302 kb on + strand at 4008.302 kb on + strand at 4008.310 kb on - strand, within yigL at 4008.350 kb on + strand, within yigL at 4008.350 kb on + strand, within yigL at 4008.393 kb on - strand, within yigL at 4008.416 kb on + strand, within yigL at 4008.430 kb on + strand, within yigL at 4008.489 kb on - strand, within yigL at 4008.495 kb on + strand, within yigL at 4008.496 kb on - strand, within yigL at 4008.505 kb on + strand, within yigL at 4008.526 kb on - strand, within yigL at 4008.526 kb on - strand, within yigL at 4008.529 kb on + strand, within yigL at 4008.572 kb on + strand, within yigL at 4008.572 kb on + strand, within yigL at 4008.621 kb on + strand, within yigL at 4008.629 kb on - strand, within yigL at 4008.629 kb on - strand, within yigL at 4008.629 kb on - strand, within yigL at 4008.632 kb on + strand, within yigL at 4008.677 kb on + strand, within yigL at 4008.677 kb on + strand, within yigL at 4008.680 kb on + strand, within yigL at 4008.688 kb on - strand, within yigL at 4008.708 kb on + strand, within yigL at 4008.754 kb on + strand, within yigL at 4008.754 kb on + strand, within yigL at 4008.754 kb on + strand, within yigL at 4008.754 kb on + strand, within yigL at 4008.762 kb on - strand, within yigL at 4008.762 kb on - strand, within yigL at 4008.903 kb on - strand, within yigL at 4008.910 kb on + strand, within yigL at 4008.918 kb on - strand, within yigL at 4008.920 kb on + strand, within yigL at 4008.920 kb on + strand, within yigL at 4008.920 kb on + strand, within yigL at 4008.968 kb on + strand at 4009.067 kb on - strand at 4009.082 kb on - strand at 4009.134 kb on - strand at 4009.176 kb on - strand at 4009.176 kb on - strand at 4009.205 kb on - strand, within yigM at 4009.275 kb on + strand, within yigM at 4009.275 kb on + strand, within yigM at 4009.275 kb on + strand, within yigM at 4009.275 kb on + strand, within yigM at 4009.275 kb on + strand, within yigM at 4009.275 kb on + strand, within yigM at 4009.283 kb on - strand, within yigM at 4009.283 kb on - strand, within yigM at 4009.311 kb on - strand, within yigM at 4009.334 kb on + strand, within yigM at 4009.334 kb on + strand, within yigM at 4009.338 kb on - strand, within yigM at 4009.348 kb on - strand, within yigM at 4009.348 kb on - strand, within yigM at 4009.406 kb on - strand, within yigM at 4009.428 kb on + strand, within yigM at 4009.434 kb on + strand, within yigM at 4009.434 kb on + strand, within yigM at 4009.451 kb on - strand, within yigM at 4009.495 kb on - strand, within yigM at 4009.495 kb on - strand, within yigM at 4009.506 kb on + strand, within yigM at 4009.524 kb on - strand, within yigM at 4009.600 kb on + strand, within yigM at 4009.772 kb on + strand, within yigM at 4009.839 kb on - strand, within yigM at 4009.839 kb on - strand, within yigM at 4009.845 kb on - strand, within yigM at 4009.845 kb on - strand, within yigM at 4009.874 kb on + strand, within yigM at 4009.904 kb on + strand at 4009.917 kb on - strand at 4009.921 kb on - strand at 4009.921 kb on - strand at 4009.947 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB Aerobic with Phenazine ethosulfate 0.0000625 mM remove 4,007,264 - +0.2 4,007,264 - -0.8 4,007,289 - -1.0 4,007,289 - -0.6 4,007,319 - pldB b3825 0.12 -0.6 4,007,345 + pldB b3825 0.15 +0.7 4,007,345 + pldB b3825 0.15 +0.8 4,007,354 - pldB b3825 0.16 -0.9 4,007,357 + pldB b3825 0.16 +0.8 4,007,357 + pldB b3825 0.16 +0.2 4,007,361 + pldB b3825 0.16 +1.1 4,007,364 - pldB b3825 0.17 -0.2 4,007,366 + pldB b3825 0.17 +0.2 4,007,379 - pldB b3825 0.18 +0.3 4,007,380 + pldB b3825 0.18 +0.2 4,007,382 + pldB b3825 0.18 +0.1 4,007,411 + pldB b3825 0.21 +1.2 4,007,421 - pldB b3825 0.22 +0.3 4,007,421 - pldB b3825 0.22 -0.2 4,007,483 + pldB b3825 0.28 +0.4 4,007,483 + pldB b3825 0.28 -0.3 4,007,491 - pldB b3825 0.29 -0.4 4,007,518 - pldB b3825 0.32 +0.2 4,007,526 - pldB b3825 0.33 +0.2 4,007,559 + pldB b3825 0.36 +0.8 4,007,559 + pldB b3825 0.36 +0.4 4,007,564 + pldB b3825 0.36 +0.5 4,007,565 + pldB b3825 0.36 -0.2 4,007,590 + pldB b3825 0.39 +0.4 4,007,614 - pldB b3825 0.41 -0.2 4,007,652 + pldB b3825 0.45 +0.0 4,007,667 + pldB b3825 0.46 +0.2 4,007,667 + pldB b3825 0.46 +0.5 4,007,731 + pldB b3825 0.53 +1.3 4,007,731 + pldB b3825 0.53 -0.6 4,007,749 - pldB b3825 0.54 -0.2 4,007,749 - pldB b3825 0.54 -0.8 4,007,811 + pldB b3825 0.60 -0.4 4,007,811 + pldB b3825 0.60 +0.6 4,007,861 + pldB b3825 0.65 +0.0 4,007,869 - pldB b3825 0.66 +0.4 4,007,869 - pldB b3825 0.66 -0.1 4,007,871 - pldB b3825 0.66 +1.0 4,007,875 + pldB b3825 0.67 +0.6 4,007,875 + pldB b3825 0.67 +1.4 4,007,881 - pldB b3825 0.67 -0.6 4,007,903 - pldB b3825 0.69 +0.2 4,008,002 - pldB b3825 0.79 -0.2 4,008,002 - pldB b3825 0.79 -0.9 4,008,017 + pldB b3825 0.81 -1.1 4,008,017 - pldB b3825 0.81 -0.5 4,008,025 - pldB b3825 0.81 -0.4 4,008,041 + pldB b3825 0.83 +0.8 4,008,054 - pldB b3825 0.84 -0.3 4,008,060 - pldB b3825 0.85 +0.3 4,008,066 + pldB b3825 0.85 +0.6 4,008,086 + pldB b3825 0.87 +0.4 4,008,086 + pldB b3825 0.87 -0.8 4,008,225 + -0.7 4,008,230 + -1.0 4,008,230 + +0.6 4,008,302 + +0.6 4,008,302 + -0.1 4,008,310 - yigL b3826 0.11 -0.7 4,008,350 + yigL b3826 0.16 -0.9 4,008,350 + yigL b3826 0.16 -0.2 4,008,393 - yigL b3826 0.21 -0.4 4,008,416 + yigL b3826 0.24 -0.3 4,008,430 + yigL b3826 0.26 -2.4 4,008,489 - yigL b3826 0.33 +0.6 4,008,495 + yigL b3826 0.34 -0.0 4,008,496 - yigL b3826 0.34 -2.0 4,008,505 + yigL b3826 0.35 +0.0 4,008,526 - yigL b3826 0.38 -0.5 4,008,526 - yigL b3826 0.38 +0.3 4,008,529 + yigL b3826 0.38 -0.2 4,008,572 + yigL b3826 0.44 -1.5 4,008,572 + yigL b3826 0.44 +0.3 4,008,621 + yigL b3826 0.50 -1.0 4,008,629 - yigL b3826 0.51 +0.4 4,008,629 - yigL b3826 0.51 +0.0 4,008,629 - yigL b3826 0.51 -2.3 4,008,632 + yigL b3826 0.51 +0.0 4,008,677 + yigL b3826 0.57 +0.8 4,008,677 + yigL b3826 0.57 +0.2 4,008,680 + yigL b3826 0.57 +0.2 4,008,688 - yigL b3826 0.58 -1.3 4,008,708 + yigL b3826 0.61 -0.2 4,008,754 + yigL b3826 0.66 -0.6 4,008,754 + yigL b3826 0.66 -1.3 4,008,754 + yigL b3826 0.66 -0.4 4,008,754 + yigL b3826 0.66 -0.1 4,008,762 - yigL b3826 0.67 +0.3 4,008,762 - yigL b3826 0.67 +0.3 4,008,903 - yigL b3826 0.85 -0.1 4,008,910 + yigL b3826 0.86 -0.8 4,008,918 - yigL b3826 0.87 +1.1 4,008,920 + yigL b3826 0.87 +0.5 4,008,920 + yigL b3826 0.87 -0.4 4,008,920 + yigL b3826 0.87 -0.3 4,008,968 + -0.4 4,009,067 - +0.4 4,009,082 - +0.1 4,009,134 - -0.7 4,009,176 - -0.3 4,009,176 - -0.0 4,009,205 - yigM b3827 0.12 +0.3 4,009,275 + yigM b3827 0.20 -0.0 4,009,275 + yigM b3827 0.20 +0.5 4,009,275 + yigM b3827 0.20 -0.4 4,009,275 + yigM b3827 0.20 -0.5 4,009,275 + yigM b3827 0.20 -2.5 4,009,275 + yigM b3827 0.20 -0.8 4,009,283 - yigM b3827 0.20 +0.6 4,009,283 - yigM b3827 0.20 +0.0 4,009,311 - yigM b3827 0.24 -0.6 4,009,334 + yigM b3827 0.26 +0.7 4,009,334 + yigM b3827 0.26 -1.4 4,009,338 - yigM b3827 0.27 -1.5 4,009,348 - yigM b3827 0.28 -0.2 4,009,348 - yigM b3827 0.28 -2.1 4,009,406 - yigM b3827 0.34 -0.7 4,009,428 + yigM b3827 0.37 -0.5 4,009,434 + yigM b3827 0.37 -0.7 4,009,434 + yigM b3827 0.37 -1.1 4,009,451 - yigM b3827 0.39 +0.1 4,009,495 - yigM b3827 0.44 -0.6 4,009,495 - yigM b3827 0.44 -0.2 4,009,506 + yigM b3827 0.45 -0.2 4,009,524 - yigM b3827 0.47 -0.1 4,009,600 + yigM b3827 0.56 -0.8 4,009,772 + yigM b3827 0.75 +0.3 4,009,839 - yigM b3827 0.82 +0.0 4,009,839 - yigM b3827 0.82 -0.5 4,009,845 - yigM b3827 0.83 -0.2 4,009,845 - yigM b3827 0.83 -0.3 4,009,874 + yigM b3827 0.86 -0.5 4,009,904 + -0.4 4,009,917 - +1.3 4,009,921 - +0.2 4,009,921 - +1.1 4,009,947 - -1.8
Or see this region's nucleotide sequence