Experiment: LB Aerobic with Phenazine ethosulfate 0.0000625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fdrA and ylbE are separated by 9 nucleotides ylbE and ylbF are separated by 10 nucleotides ylbF and ybcF overlap by 4 nucleotides
b0518: fdrA - membrane protein FdrA (NCBI), at 545,904 to 547,571
fdrA
b4572: ylbE - no description, at 547,581 to 548,839
ylbE
b0520: ylbF - hypothetical protein (NCBI), at 548,850 to 549,665
ylbF
b0521: ybcF - predicted carbamate kinase (NCBI), at 549,662 to 550,555
ybcF
Position (kb) 547
548
549 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5
6
7
8 at 546.609 kb on + strand, within fdrA at 546.609 kb on + strand, within fdrA at 546.693 kb on + strand, within fdrA at 546.957 kb on + strand, within fdrA at 547.026 kb on + strand, within fdrA at 547.026 kb on + strand, within fdrA at 547.030 kb on - strand, within fdrA at 547.054 kb on - strand, within fdrA at 547.054 kb on - strand, within fdrA at 547.081 kb on + strand, within fdrA at 547.406 kb on + strand at 547.406 kb on + strand at 547.462 kb on + strand at 547.591 kb on - strand at 547.628 kb on - strand at 547.638 kb on + strand at 547.644 kb on - strand at 547.644 kb on - strand at 547.648 kb on + strand at 547.729 kb on - strand, within ylbE at 547.729 kb on - strand, within ylbE at 547.895 kb on - strand, within ylbE at 548.009 kb on + strand, within ylbE at 548.151 kb on - strand, within ylbE at 548.165 kb on + strand, within ylbE at 548.165 kb on + strand, within ylbE at 548.208 kb on - strand, within ylbE at 548.208 kb on - strand, within ylbE at 548.506 kb on + strand, within ylbE at 548.533 kb on + strand, within ylbE at 548.533 kb on + strand, within ylbE at 548.700 kb on + strand, within ylbE at 548.708 kb on - strand, within ylbE at 548.713 kb on - strand, within ylbE at 548.718 kb on - strand at 548.755 kb on + strand at 548.833 kb on + strand at 549.010 kb on + strand, within ylbF at 549.010 kb on + strand, within ylbF at 549.046 kb on - strand, within ylbF at 549.046 kb on - strand, within ylbF at 549.219 kb on + strand, within ylbF at 549.354 kb on - strand, within ylbF at 549.354 kb on - strand, within ylbF at 549.392 kb on + strand, within ylbF at 549.450 kb on + strand, within ylbF at 549.450 kb on + strand, within ylbF at 549.458 kb on + strand, within ylbF at 549.488 kb on + strand, within ylbF at 549.491 kb on - strand, within ylbF at 549.534 kb on + strand, within ylbF at 549.625 kb on + strand at 549.695 kb on + strand at 549.755 kb on + strand, within ybcF at 549.755 kb on + strand, within ybcF at 549.764 kb on + strand, within ybcF at 549.811 kb on - strand, within ybcF
Per-strain Table
Position Strand Gene LocusTag Fraction LB Aerobic with Phenazine ethosulfate 0.0000625 mM remove 546,609 + fdrA b0518 0.42 -0.7 546,609 + fdrA b0518 0.42 +0.6 546,693 + fdrA b0518 0.47 -0.1 546,957 + fdrA b0518 0.63 -0.2 547,026 + fdrA b0518 0.67 -1.3 547,026 + fdrA b0518 0.67 -1.1 547,030 - fdrA b0518 0.68 +0.3 547,054 - fdrA b0518 0.69 -0.4 547,054 - fdrA b0518 0.69 +0.7 547,081 + fdrA b0518 0.71 +0.3 547,406 + -1.2 547,406 + +0.3 547,462 + -1.0 547,591 - -2.3 547,628 - -0.7 547,638 + +0.3 547,644 - +0.6 547,644 - +0.8 547,648 + +1.0 547,729 - ylbE b4572 0.12 +6.2 547,729 - ylbE b4572 0.12 +7.9 547,895 - ylbE b4572 0.25 -0.2 548,009 + ylbE b4572 0.34 +0.3 548,151 - ylbE b4572 0.45 +0.2 548,165 + ylbE b4572 0.46 +0.3 548,165 + ylbE b4572 0.46 -0.1 548,208 - ylbE b4572 0.50 +0.7 548,208 - ylbE b4572 0.50 +0.5 548,506 + ylbE b4572 0.73 -0.5 548,533 + ylbE b4572 0.76 -1.0 548,533 + ylbE b4572 0.76 +0.8 548,700 + ylbE b4572 0.89 -0.5 548,708 - ylbE b4572 0.90 +0.6 548,713 - ylbE b4572 0.90 +0.0 548,718 - -0.3 548,755 + -0.5 548,833 + -0.1 549,010 + ylbF b0520 0.20 +0.1 549,010 + ylbF b0520 0.20 +0.2 549,046 - ylbF b0520 0.24 +0.1 549,046 - ylbF b0520 0.24 +1.1 549,219 + ylbF b0520 0.45 -1.5 549,354 - ylbF b0520 0.62 -0.5 549,354 - ylbF b0520 0.62 +0.2 549,392 + ylbF b0520 0.66 +0.9 549,450 + ylbF b0520 0.74 -2.6 549,450 + ylbF b0520 0.74 +1.2 549,458 + ylbF b0520 0.75 +0.2 549,488 + ylbF b0520 0.78 -0.1 549,491 - ylbF b0520 0.79 -0.3 549,534 + ylbF b0520 0.84 +0.6 549,625 + +0.2 549,695 + +0.1 549,755 + ybcF b0521 0.10 -0.4 549,755 + ybcF b0521 0.10 +0.1 549,764 + ybcF b0521 0.11 -0.9 549,811 - ybcF b0521 0.17 -0.5
Or see this region's nucleotide sequence