Experiment: LB Aerobic with Phenazine ethosulfate 0.0000625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yjjZ and leuV are separated by 38 nucleotides leuV and leuP are separated by 34 nucleotides leuP and leuQ are separated by 28 nucleotides leuQ and rsmC are separated by 267 nucleotides rsmC and holD are separated by 102 nucleotides holD and rimI overlap by 32 nucleotides rimI and yjjG are separated by 14 nucleotides
b4567: yjjZ - hypothetical protein (NCBI), at 4,603,827 to 4,604,063
yjjZ
b4368: leuV - tRNA-Leu (NCBI), at 4,604,102 to 4,604,188
leuV
b4369: leuP - tRNA-Leu (NCBI), at 4,604,223 to 4,604,309
leuP
b4370: leuQ - tRNA-Leu (NCBI), at 4,604,338 to 4,604,424
leuQ
b4371: rsmC - 16S ribosomal RNA m2G1207 methyltransferase (NCBI), at 4,604,692 to 4,605,723
rsmC
b4372: holD - DNA polymerase III subunit psi (NCBI), at 4,605,826 to 4,606,239
holD
b4373: rimI - ribosomal-protein-alanine N-acetyltransferase (NCBI), at 4,606,208 to 4,606,654
rimI
b4374: yjjG - nucleotidase (NCBI), at 4,606,669 to 4,607,346
yjjG
Position (kb)
4604
4605
4606 Strain fitness (log2 ratio)
-1
0
1
2 at 4603.847 kb on - strand at 4603.872 kb on + strand, within yjjZ at 4603.882 kb on + strand, within yjjZ at 4603.927 kb on - strand, within yjjZ at 4603.927 kb on - strand, within yjjZ at 4603.935 kb on - strand, within yjjZ at 4603.937 kb on + strand, within yjjZ at 4604.119 kb on - strand, within leuV at 4604.280 kb on - strand, within leuP at 4604.280 kb on - strand, within leuP at 4604.377 kb on - strand, within leuQ at 4604.377 kb on - strand, within leuQ at 4604.381 kb on - strand, within leuQ at 4604.387 kb on + strand, within leuQ at 4604.410 kb on + strand, within leuQ at 4604.467 kb on + strand at 4604.467 kb on + strand at 4604.467 kb on + strand at 4604.508 kb on - strand at 4604.618 kb on - strand at 4604.653 kb on - strand at 4604.732 kb on + strand at 4604.732 kb on + strand at 4604.745 kb on + strand at 4604.758 kb on + strand at 4604.851 kb on + strand, within rsmC at 4604.856 kb on + strand, within rsmC at 4604.884 kb on + strand, within rsmC at 4604.884 kb on + strand, within rsmC at 4604.927 kb on - strand, within rsmC at 4604.927 kb on - strand, within rsmC at 4605.041 kb on + strand, within rsmC at 4605.138 kb on + strand, within rsmC at 4605.178 kb on + strand, within rsmC at 4605.238 kb on + strand, within rsmC at 4605.238 kb on + strand, within rsmC at 4605.298 kb on - strand, within rsmC at 4605.304 kb on + strand, within rsmC at 4605.407 kb on + strand, within rsmC at 4605.453 kb on - strand, within rsmC at 4605.484 kb on - strand, within rsmC at 4605.484 kb on - strand, within rsmC at 4605.533 kb on - strand, within rsmC at 4605.564 kb on - strand, within rsmC at 4605.579 kb on - strand, within rsmC at 4605.579 kb on - strand, within rsmC at 4605.610 kb on + strand, within rsmC at 4605.662 kb on - strand at 4605.696 kb on - strand at 4605.696 kb on - strand at 4605.740 kb on - strand at 4605.783 kb on - strand at 4605.858 kb on + strand at 4606.288 kb on - strand, within rimI at 4606.288 kb on - strand, within rimI at 4606.309 kb on + strand, within rimI at 4606.309 kb on + strand, within rimI at 4606.385 kb on - strand, within rimI at 4606.611 kb on + strand at 4606.636 kb on - strand at 4606.652 kb on - strand at 4606.655 kb on - strand at 4606.668 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB Aerobic with Phenazine ethosulfate 0.0000625 mM remove 4,603,847 - -0.2 4,603,872 + yjjZ b4567 0.19 +0.7 4,603,882 + yjjZ b4567 0.23 -0.1 4,603,927 - yjjZ b4567 0.42 -0.5 4,603,927 - yjjZ b4567 0.42 +0.2 4,603,935 - yjjZ b4567 0.46 -1.0 4,603,937 + yjjZ b4567 0.46 -0.4 4,604,119 - leuV b4368 0.20 -0.8 4,604,280 - leuP b4369 0.66 -0.0 4,604,280 - leuP b4369 0.66 -0.4 4,604,377 - leuQ b4370 0.45 +0.8 4,604,377 - leuQ b4370 0.45 +0.6 4,604,381 - leuQ b4370 0.49 -0.5 4,604,387 + leuQ b4370 0.56 -0.0 4,604,410 + leuQ b4370 0.83 +0.4 4,604,467 + -0.8 4,604,467 + +0.6 4,604,467 + -0.3 4,604,508 - -0.2 4,604,618 - -0.4 4,604,653 - +0.1 4,604,732 + +0.5 4,604,732 + +0.6 4,604,745 + +1.0 4,604,758 + +1.1 4,604,851 + rsmC b4371 0.15 +0.7 4,604,856 + rsmC b4371 0.16 +0.8 4,604,884 + rsmC b4371 0.19 +0.6 4,604,884 + rsmC b4371 0.19 +0.6 4,604,927 - rsmC b4371 0.23 +1.5 4,604,927 - rsmC b4371 0.23 +0.6 4,605,041 + rsmC b4371 0.34 +0.8 4,605,138 + rsmC b4371 0.43 -0.7 4,605,178 + rsmC b4371 0.47 +0.6 4,605,238 + rsmC b4371 0.53 +0.9 4,605,238 + rsmC b4371 0.53 -0.5 4,605,298 - rsmC b4371 0.59 +1.2 4,605,304 + rsmC b4371 0.59 +0.7 4,605,407 + rsmC b4371 0.69 +1.2 4,605,453 - rsmC b4371 0.74 +0.2 4,605,484 - rsmC b4371 0.77 +0.3 4,605,484 - rsmC b4371 0.77 +1.1 4,605,533 - rsmC b4371 0.81 +1.5 4,605,564 - rsmC b4371 0.84 +1.6 4,605,579 - rsmC b4371 0.86 -0.0 4,605,579 - rsmC b4371 0.86 +1.7 4,605,610 + rsmC b4371 0.89 +0.9 4,605,662 - +0.8 4,605,696 - -0.1 4,605,696 - +1.5 4,605,740 - -1.1 4,605,783 - -0.3 4,605,858 + -0.8 4,606,288 - rimI b4373 0.18 +0.7 4,606,288 - rimI b4373 0.18 +1.9 4,606,309 + rimI b4373 0.23 +1.5 4,606,309 + rimI b4373 0.23 +0.8 4,606,385 - rimI b4373 0.40 +1.3 4,606,611 + +1.4 4,606,636 - +0.7 4,606,652 - +0.1 4,606,655 - -0.0 4,606,668 + +0.3
Or see this region's nucleotide sequence