Strain Fitness in Escherichia coli BW25113 around b3345

Experiment: LB Aerobic with Phenazine ethosulfate 0.0000625 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntrpsL and yheL are separated by 125 nucleotidesyheL and yheM are separated by 7 nucleotidesyheM and yheN overlap by 1 nucleotidesyheN and yheO overlap by 1 nucleotidesyheO and fkpA are separated by 166 nucleotides b3342: rpsL - 30S ribosomal protein S12 (NCBI), at 3,472,200 to 3,472,574 rpsL b3343: yheL - predicted intracellular sulfur oxidation protein (NCBI), at 3,472,700 to 3,472,987 yheL b3344: yheM - hypothetical protein (NCBI), at 3,472,995 to 3,473,354 yheM b3345: yheN - hypothetical protein (NCBI), at 3,473,354 to 3,473,740 yheN b3346: yheO - orf, hypothetical protein (VIMSS), at 3,473,740 to 3,474,462 yheO b3347: fkpA - FKBP-type peptidyl-prolyl cis-trans isomerase (rotamase) (NCBI), at 3,474,629 to 3,475,441 fkpA Position (kb) 3473 3474Strain fitness (log2 ratio) -2 -1 0 1 2at 3472.640 kb on - strandat 3472.640 kb on - strandat 3472.681 kb on + strandat 3472.681 kb on - strandat 3472.987 kb on - strandat 3472.987 kb on - strandat 3472.999 kb on - strandat 3473.352 kb on + strandat 3473.352 kb on + strandat 3473.745 kb on + strandat 3473.778 kb on + strandat 3473.784 kb on + strandat 3473.788 kb on + strandat 3473.788 kb on + strandat 3473.788 kb on + strandat 3473.790 kb on + strandat 3473.798 kb on - strandat 3473.800 kb on + strandat 3473.809 kb on - strandat 3473.810 kb on - strandat 3473.913 kb on - strand, within yheOat 3473.913 kb on - strand, within yheOat 3473.920 kb on - strand, within yheOat 3473.931 kb on - strand, within yheOat 3474.071 kb on - strand, within yheOat 3474.072 kb on - strand, within yheOat 3474.097 kb on - strand, within yheOat 3474.122 kb on + strand, within yheOat 3474.181 kb on + strand, within yheOat 3474.187 kb on - strand, within yheOat 3474.205 kb on - strand, within yheOat 3474.207 kb on + strand, within yheOat 3474.207 kb on - strand, within yheOat 3474.214 kb on - strand, within yheOat 3474.251 kb on + strand, within yheOat 3474.251 kb on + strand, within yheOat 3474.257 kb on - strand, within yheOat 3474.257 kb on - strand, within yheOat 3474.272 kb on - strand, within yheOat 3474.350 kb on - strand, within yheOat 3474.350 kb on - strand, within yheOat 3474.360 kb on - strand, within yheOat 3474.360 kb on - strand, within yheOat 3474.360 kb on - strand, within yheOat 3474.404 kb on - strandat 3474.485 kb on - strandat 3474.507 kb on - strandat 3474.520 kb on - strandat 3474.520 kb on - strandat 3474.536 kb on + strandat 3474.536 kb on + strandat 3474.578 kb on - strandat 3474.578 kb on - strandat 3474.664 kb on - strandat 3474.675 kb on + strandat 3474.689 kb on - strandat 3474.701 kb on - strandat 3474.702 kb on - strandat 3474.704 kb on + strandat 3474.722 kb on - strand, within fkpAat 3474.735 kb on - strand, within fkpA

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Aerobic with Phenazine ethosulfate 0.0000625 mM
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3,472,640 - +0.7
3,472,640 - -1.3
3,472,681 + +0.0
3,472,681 - +1.0
3,472,987 - -1.6
3,472,987 - -0.4
3,472,999 - +0.3
3,473,352 + +0.3
3,473,352 + +0.2
3,473,745 + +0.8
3,473,778 + -0.2
3,473,784 + +2.3
3,473,788 + -0.7
3,473,788 + +0.8
3,473,788 + +0.0
3,473,790 + +1.3
3,473,798 - -0.9
3,473,800 + +0.3
3,473,809 - -0.3
3,473,810 - -0.0
3,473,913 - yheO b3346 0.24 +0.6
3,473,913 - yheO b3346 0.24 -1.2
3,473,920 - yheO b3346 0.25 -0.3
3,473,931 - yheO b3346 0.26 +0.5
3,474,071 - yheO b3346 0.46 -1.3
3,474,072 - yheO b3346 0.46 +0.0
3,474,097 - yheO b3346 0.49 -0.2
3,474,122 + yheO b3346 0.53 -0.1
3,474,181 + yheO b3346 0.61 +2.3
3,474,187 - yheO b3346 0.62 +0.3
3,474,205 - yheO b3346 0.64 -0.0
3,474,207 + yheO b3346 0.65 +0.1
3,474,207 - yheO b3346 0.65 +0.0
3,474,214 - yheO b3346 0.66 -0.2
3,474,251 + yheO b3346 0.71 +0.2
3,474,251 + yheO b3346 0.71 +0.5
3,474,257 - yheO b3346 0.72 +0.0
3,474,257 - yheO b3346 0.72 +0.5
3,474,272 - yheO b3346 0.74 -0.4
3,474,350 - yheO b3346 0.84 -0.1
3,474,350 - yheO b3346 0.84 +0.2
3,474,360 - yheO b3346 0.86 -0.6
3,474,360 - yheO b3346 0.86 +0.2
3,474,360 - yheO b3346 0.86 -1.0
3,474,404 - -0.7
3,474,485 - -0.2
3,474,507 - +1.2
3,474,520 - +0.3
3,474,520 - +0.1
3,474,536 + +1.1
3,474,536 + -1.6
3,474,578 - +0.6
3,474,578 - +1.1
3,474,664 - +0.7
3,474,675 + -0.4
3,474,689 - +0.7
3,474,701 - +0.1
3,474,702 - -2.5
3,474,704 + +0.9
3,474,722 - fkpA b3347 0.11 +0.4
3,474,735 - fkpA b3347 0.13 +0.3

Or see this region's nucleotide sequence