Experiment: LB Aerobic with Phenazine ethosulfate 0.0000625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pepB and yfhJ are separated by 177 nucleotides yfhJ and fdx are separated by 11 nucleotides fdx and hscA are separated by 1 nucleotides hscA and hscB are separated by 16 nucleotides hscB and iscA are separated by 95 nucleotides iscA and iscU are separated by 16 nucleotides
b2523: pepB - putative peptidase (VIMSS), at 2,653,097 to 2,654,380
pepB
b2524: yfhJ - hypothetical protein (NCBI), at 2,654,558 to 2,654,758
yfhJ
b2525: fdx - [2Fe-2S] ferredoxin (NCBI), at 2,654,770 to 2,655,105
fdx
b2526: hscA - chaperone protein HscA (NCBI), at 2,655,107 to 2,656,957
hscA
b2527: hscB - co-chaperone HscB (NCBI), at 2,656,974 to 2,657,489
hscB
b2528: iscA - iron-sulfur cluster assembly protein (NCBI), at 2,657,585 to 2,657,908
iscA
b2529: iscU - scaffold protein (NCBI), at 2,657,925 to 2,658,311
iscU
Position (kb)
2655
2656
2657 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2654.143 kb on + strand, within pepB at 2654.144 kb on + strand, within pepB at 2654.163 kb on + strand, within pepB at 2654.222 kb on + strand, within pepB at 2654.271 kb on - strand at 2654.271 kb on - strand at 2654.404 kb on - strand at 2654.451 kb on + strand at 2654.451 kb on + strand at 2654.460 kb on + strand at 2654.500 kb on - strand at 2654.754 kb on - strand at 2654.884 kb on + strand, within fdx at 2655.131 kb on - strand at 2655.278 kb on - strand at 2656.964 kb on - strand at 2657.633 kb on - strand, within iscA at 2657.795 kb on - strand, within iscA at 2657.873 kb on - strand, within iscA at 2657.886 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB Aerobic with Phenazine ethosulfate 0.0000625 mM remove 2,654,143 + pepB b2523 0.81 +1.2 2,654,144 + pepB b2523 0.82 +0.5 2,654,163 + pepB b2523 0.83 +0.1 2,654,222 + pepB b2523 0.88 +0.6 2,654,271 - +0.9 2,654,271 - -1.8 2,654,404 - -0.3 2,654,451 + +0.4 2,654,451 + +1.4 2,654,460 + -0.1 2,654,500 - +1.5 2,654,754 - +0.3 2,654,884 + fdx b2525 0.34 +0.9 2,655,131 - +0.4 2,655,278 - +1.9 2,656,964 - +0.6 2,657,633 - iscA b2528 0.15 +0.7 2,657,795 - iscA b2528 0.65 -0.7 2,657,873 - iscA b2528 0.89 +0.7 2,657,886 - +2.5
Or see this region's nucleotide sequence