Strain Fitness in Escherichia coli BW25113 around b2526

Experiment: LB Aerobic with Phenazine ethosulfate 0.0000625 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntpepB and yfhJ are separated by 177 nucleotidesyfhJ and fdx are separated by 11 nucleotidesfdx and hscA are separated by 1 nucleotideshscA and hscB are separated by 16 nucleotideshscB and iscA are separated by 95 nucleotidesiscA and iscU are separated by 16 nucleotides b2523: pepB - putative peptidase (VIMSS), at 2,653,097 to 2,654,380 pepB b2524: yfhJ - hypothetical protein (NCBI), at 2,654,558 to 2,654,758 yfhJ b2525: fdx - [2Fe-2S] ferredoxin (NCBI), at 2,654,770 to 2,655,105 fdx b2526: hscA - chaperone protein HscA (NCBI), at 2,655,107 to 2,656,957 hscA b2527: hscB - co-chaperone HscB (NCBI), at 2,656,974 to 2,657,489 hscB b2528: iscA - iron-sulfur cluster assembly protein (NCBI), at 2,657,585 to 2,657,908 iscA b2529: iscU - scaffold protein (NCBI), at 2,657,925 to 2,658,311 iscU Position (kb) 2655 2656 2657Strain fitness (log2 ratio) -2 -1 0 1 2at 2654.143 kb on + strand, within pepBat 2654.144 kb on + strand, within pepBat 2654.163 kb on + strand, within pepBat 2654.222 kb on + strand, within pepBat 2654.271 kb on - strandat 2654.271 kb on - strandat 2654.404 kb on - strandat 2654.451 kb on + strandat 2654.451 kb on + strandat 2654.460 kb on + strandat 2654.500 kb on - strandat 2654.754 kb on - strandat 2654.884 kb on + strand, within fdxat 2655.131 kb on - strandat 2655.278 kb on - strandat 2656.964 kb on - strandat 2657.633 kb on - strand, within iscAat 2657.795 kb on - strand, within iscAat 2657.873 kb on - strand, within iscAat 2657.886 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Aerobic with Phenazine ethosulfate 0.0000625 mM
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2,654,143 + pepB b2523 0.81 +1.2
2,654,144 + pepB b2523 0.82 +0.5
2,654,163 + pepB b2523 0.83 +0.1
2,654,222 + pepB b2523 0.88 +0.6
2,654,271 - +0.9
2,654,271 - -1.8
2,654,404 - -0.3
2,654,451 + +0.4
2,654,451 + +1.4
2,654,460 + -0.1
2,654,500 - +1.5
2,654,754 - +0.3
2,654,884 + fdx b2525 0.34 +0.9
2,655,131 - +0.4
2,655,278 - +1.9
2,656,964 - +0.6
2,657,633 - iscA b2528 0.15 +0.7
2,657,795 - iscA b2528 0.65 -0.7
2,657,873 - iscA b2528 0.89 +0.7
2,657,886 - +2.5

Or see this region's nucleotide sequence