Strain Fitness in Escherichia coli BW25113 around b1111

Experiment: LB Aerobic with Phenazine ethosulfate 0.0000625 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntndh and ycfJ are separated by 209 nucleotidesycfJ and ycfQ are separated by 61 nucleotidesycfQ and ycfR are separated by 240 nucleotidesycfR and ycfS are separated by 81 nucleotides b1109: ndh - respiratory NADH dehydrogenase 2/cupric reductase (NCBI), at 1,165,308 to 1,166,612 ndh b1110: ycfJ - hypothetical protein (NCBI), at 1,166,822 to 1,167,361 ycfJ b1111: ycfQ - orf, hypothetical protein (VIMSS), at 1,167,423 to 1,168,055 ycfQ b1112: ycfR - hypothetical protein (NCBI), at 1,168,296 to 1,168,553 ycfR b1113: ycfS - hypothetical protein (NCBI), at 1,168,635 to 1,169,597 ycfS Position (kb) 1167 1168 1169Strain fitness (log2 ratio) -2 -1 0 1 2at 1166.478 kb on - strand, within ndhat 1166.501 kb on - strandat 1166.526 kb on + strandat 1166.573 kb on - strandat 1166.623 kb on - strandat 1166.655 kb on + strandat 1166.661 kb on - strandat 1166.824 kb on + strandat 1166.972 kb on + strand, within ycfJat 1166.972 kb on + strand, within ycfJat 1166.972 kb on + strand, within ycfJat 1166.972 kb on + strand, within ycfJat 1166.980 kb on + strand, within ycfJat 1167.051 kb on + strand, within ycfJat 1167.051 kb on + strand, within ycfJat 1167.125 kb on + strand, within ycfJat 1167.132 kb on + strand, within ycfJat 1167.132 kb on + strand, within ycfJat 1167.243 kb on + strand, within ycfJat 1167.243 kb on + strand, within ycfJat 1167.243 kb on + strand, within ycfJat 1167.251 kb on - strand, within ycfJat 1167.263 kb on - strand, within ycfJat 1167.263 kb on - strand, within ycfJat 1167.362 kb on - strandat 1167.364 kb on + strandat 1167.364 kb on + strandat 1167.640 kb on + strand, within ycfQat 1167.840 kb on + strand, within ycfQat 1167.840 kb on - strand, within ycfQat 1167.949 kb on - strand, within ycfQat 1168.045 kb on - strandat 1168.045 kb on - strandat 1168.075 kb on - strandat 1168.075 kb on - strandat 1168.746 kb on + strand, within ycfSat 1168.766 kb on + strand, within ycfSat 1168.802 kb on + strand, within ycfSat 1168.805 kb on + strand, within ycfSat 1168.870 kb on + strand, within ycfSat 1168.925 kb on - strand, within ycfSat 1169.013 kb on + strand, within ycfS

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Aerobic with Phenazine ethosulfate 0.0000625 mM
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1,166,478 - ndh b1109 0.90 +0.2
1,166,501 - +1.4
1,166,526 + -0.6
1,166,573 - -1.4
1,166,623 - -0.6
1,166,655 + -0.2
1,166,661 - -0.2
1,166,824 + -1.5
1,166,972 + ycfJ b1110 0.28 -0.8
1,166,972 + ycfJ b1110 0.28 -0.1
1,166,972 + ycfJ b1110 0.28 +0.2
1,166,972 + ycfJ b1110 0.28 +0.5
1,166,980 + ycfJ b1110 0.29 -0.2
1,167,051 + ycfJ b1110 0.42 +1.2
1,167,051 + ycfJ b1110 0.42 +0.4
1,167,125 + ycfJ b1110 0.56 +0.0
1,167,132 + ycfJ b1110 0.57 +0.2
1,167,132 + ycfJ b1110 0.57 -1.8
1,167,243 + ycfJ b1110 0.78 -0.7
1,167,243 + ycfJ b1110 0.78 +1.3
1,167,243 + ycfJ b1110 0.78 +0.7
1,167,251 - ycfJ b1110 0.79 +0.4
1,167,263 - ycfJ b1110 0.82 +1.0
1,167,263 - ycfJ b1110 0.82 +1.5
1,167,362 - +1.2
1,167,364 + +0.3
1,167,364 + +0.2
1,167,640 + ycfQ b1111 0.34 +1.2
1,167,840 + ycfQ b1111 0.66 +0.2
1,167,840 - ycfQ b1111 0.66 +0.7
1,167,949 - ycfQ b1111 0.83 +1.4
1,168,045 - +1.8
1,168,045 - +0.6
1,168,075 - -0.1
1,168,075 - +0.8
1,168,746 + ycfS b1113 0.12 +0.3
1,168,766 + ycfS b1113 0.14 +0.9
1,168,802 + ycfS b1113 0.17 +1.4
1,168,805 + ycfS b1113 0.18 +1.0
1,168,870 + ycfS b1113 0.24 +1.9
1,168,925 - ycfS b1113 0.30 -0.1
1,169,013 + ycfS b1113 0.39 -0.5

Or see this region's nucleotide sequence