Experiment: LB Aerobic with Ramoplanin 0.000125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt thiC and rsd are separated by 232 nucleotides rsd and nudC are separated by 94 nucleotides nudC and hemE are separated by 39 nucleotides
b3994: thiC - thiamine biosynthesis protein ThiC (NCBI), at 4,192,227 to 4,194,122
thiC
b3995: rsd - stationary phase protein, binds sigma 70 RNA polymerase subunit (NCBI), at 4,194,355 to 4,194,831
rsd
b3996: nudC - NADH pyrophosphatase (NCBI), at 4,194,926 to 4,195,699
nudC
b3997: hemE - uroporphyrinogen decarboxylase (NCBI), at 4,195,739 to 4,196,803
hemE
Position (kb)
4194
4195
4196 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4193.952 kb on + strand at 4193.970 kb on + strand at 4193.980 kb on - strand at 4193.980 kb on - strand at 4194.014 kb on - strand at 4194.054 kb on + strand at 4194.061 kb on - strand at 4194.061 kb on - strand at 4194.118 kb on + strand at 4194.164 kb on + strand at 4194.165 kb on + strand at 4194.165 kb on + strand at 4194.173 kb on - strand at 4194.515 kb on - strand, within rsd at 4194.516 kb on + strand, within rsd at 4194.602 kb on - strand, within rsd at 4194.602 kb on - strand, within rsd at 4194.726 kb on + strand, within rsd at 4194.726 kb on + strand, within rsd at 4194.728 kb on - strand, within rsd at 4194.728 kb on - strand, within rsd at 4194.732 kb on + strand, within rsd at 4194.732 kb on + strand, within rsd at 4194.738 kb on - strand, within rsd at 4194.751 kb on + strand, within rsd at 4194.755 kb on + strand, within rsd at 4194.812 kb on - strand at 4194.822 kb on + strand at 4194.905 kb on + strand at 4194.911 kb on - strand at 4194.923 kb on + strand at 4194.923 kb on + strand at 4194.965 kb on + strand at 4195.102 kb on - strand, within nudC at 4195.102 kb on - strand, within nudC at 4195.124 kb on - strand, within nudC at 4195.125 kb on + strand, within nudC at 4195.143 kb on - strand, within nudC at 4195.178 kb on + strand, within nudC at 4195.178 kb on + strand, within nudC at 4195.216 kb on - strand, within nudC at 4195.216 kb on - strand, within nudC at 4195.218 kb on + strand, within nudC at 4195.218 kb on + strand, within nudC at 4195.260 kb on + strand, within nudC at 4195.260 kb on + strand, within nudC at 4195.270 kb on + strand, within nudC at 4195.278 kb on - strand, within nudC at 4195.296 kb on - strand, within nudC at 4195.305 kb on - strand, within nudC at 4195.312 kb on + strand, within nudC at 4195.312 kb on + strand, within nudC at 4195.315 kb on - strand, within nudC at 4195.355 kb on + strand, within nudC at 4195.380 kb on - strand, within nudC at 4195.386 kb on + strand, within nudC at 4195.389 kb on - strand, within nudC at 4195.391 kb on - strand, within nudC at 4195.391 kb on - strand, within nudC at 4195.394 kb on - strand, within nudC at 4195.448 kb on - strand, within nudC at 4195.448 kb on - strand, within nudC at 4195.449 kb on + strand, within nudC at 4195.457 kb on - strand, within nudC at 4195.457 kb on - strand, within nudC at 4195.472 kb on - strand, within nudC at 4195.494 kb on + strand, within nudC at 4195.494 kb on - strand, within nudC at 4195.607 kb on - strand, within nudC at 4195.619 kb on + strand, within nudC at 4195.619 kb on + strand, within nudC at 4195.641 kb on - strand at 4195.641 kb on - strand at 4195.651 kb on - strand at 4195.661 kb on - strand at 4195.661 kb on - strand at 4195.665 kb on + strand at 4195.670 kb on + strand at 4195.680 kb on + strand at 4195.686 kb on - strand at 4195.688 kb on + strand at 4195.688 kb on + strand at 4195.688 kb on + strand at 4195.690 kb on + strand at 4195.699 kb on + strand at 4195.699 kb on + strand at 4195.699 kb on + strand at 4195.707 kb on - strand at 4195.707 kb on - strand at 4195.707 kb on - strand at 4195.707 kb on - strand at 4195.707 kb on - strand at 4195.707 kb on - strand at 4195.707 kb on - strand at 4195.709 kb on - strand at 4195.723 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB Aerobic with Ramoplanin 0.000125 mM remove 4,193,952 + -1.3 4,193,970 + +0.0 4,193,980 - -0.4 4,193,980 - -0.7 4,194,014 - +1.0 4,194,054 + +1.6 4,194,061 - -0.3 4,194,061 - -0.2 4,194,118 + -0.7 4,194,164 + -0.3 4,194,165 + +0.3 4,194,165 + +0.5 4,194,173 - +0.4 4,194,515 - rsd b3995 0.34 -0.2 4,194,516 + rsd b3995 0.34 +0.7 4,194,602 - rsd b3995 0.52 -1.5 4,194,602 - rsd b3995 0.52 +0.7 4,194,726 + rsd b3995 0.78 +0.9 4,194,726 + rsd b3995 0.78 -1.4 4,194,728 - rsd b3995 0.78 +0.0 4,194,728 - rsd b3995 0.78 +0.2 4,194,732 + rsd b3995 0.79 -1.1 4,194,732 + rsd b3995 0.79 -1.0 4,194,738 - rsd b3995 0.80 -0.4 4,194,751 + rsd b3995 0.83 -1.4 4,194,755 + rsd b3995 0.84 +0.7 4,194,812 - +0.3 4,194,822 + -1.5 4,194,905 + +0.5 4,194,911 - -1.0 4,194,923 + -1.2 4,194,923 + +0.8 4,194,965 + +1.2 4,195,102 - nudC b3996 0.23 -0.4 4,195,102 - nudC b3996 0.23 +1.5 4,195,124 - nudC b3996 0.26 -0.1 4,195,125 + nudC b3996 0.26 +1.1 4,195,143 - nudC b3996 0.28 +0.6 4,195,178 + nudC b3996 0.33 +1.7 4,195,178 + nudC b3996 0.33 +1.8 4,195,216 - nudC b3996 0.37 +0.4 4,195,216 - nudC b3996 0.37 +1.4 4,195,218 + nudC b3996 0.38 -1.4 4,195,218 + nudC b3996 0.38 +2.4 4,195,260 + nudC b3996 0.43 -0.1 4,195,260 + nudC b3996 0.43 +0.0 4,195,270 + nudC b3996 0.44 -0.6 4,195,278 - nudC b3996 0.45 +0.8 4,195,296 - nudC b3996 0.48 +0.8 4,195,305 - nudC b3996 0.49 +0.6 4,195,312 + nudC b3996 0.50 +0.6 4,195,312 + nudC b3996 0.50 +1.7 4,195,315 - nudC b3996 0.50 -0.8 4,195,355 + nudC b3996 0.55 -2.4 4,195,380 - nudC b3996 0.59 +0.4 4,195,386 + nudC b3996 0.59 +0.8 4,195,389 - nudC b3996 0.60 +1.2 4,195,391 - nudC b3996 0.60 +1.6 4,195,391 - nudC b3996 0.60 +0.2 4,195,394 - nudC b3996 0.60 +0.4 4,195,448 - nudC b3996 0.67 +0.4 4,195,448 - nudC b3996 0.67 -0.7 4,195,449 + nudC b3996 0.68 +0.9 4,195,457 - nudC b3996 0.69 -1.0 4,195,457 - nudC b3996 0.69 +0.3 4,195,472 - nudC b3996 0.71 +1.3 4,195,494 + nudC b3996 0.73 +1.0 4,195,494 - nudC b3996 0.73 +1.6 4,195,607 - nudC b3996 0.88 +1.0 4,195,619 + nudC b3996 0.90 +1.1 4,195,619 + nudC b3996 0.90 +1.0 4,195,641 - +0.5 4,195,641 - +1.2 4,195,651 - -0.4 4,195,661 - -0.6 4,195,661 - -0.1 4,195,665 + +0.2 4,195,670 + +0.7 4,195,680 + -1.0 4,195,686 - +1.5 4,195,688 + -0.2 4,195,688 + +0.6 4,195,688 + +1.4 4,195,690 + -0.5 4,195,699 + -0.2 4,195,699 + +0.7 4,195,699 + +0.3 4,195,707 - +1.0 4,195,707 - -2.9 4,195,707 - +1.5 4,195,707 - -0.1 4,195,707 - +2.7 4,195,707 - -0.0 4,195,707 - -0.2 4,195,709 - -2.7 4,195,723 - -0.6
Or see this region's nucleotide sequence