Experiment: LB Aerobic with Ramoplanin 0.000125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fecC and fecB overlap by 4 nucleotides fecB and fecA are separated by 44 nucleotides fecA and fecR are separated by 86 nucleotides
b4289: fecC - KpLE2 phage-like element; iron-dicitrate transporter subunit (NCBI), at 4,510,434 to 4,511,432
fecC
b4290: fecB - citrate-dependent iron transport, periplasmic protein (VIMSS), at 4,511,429 to 4,512,331
fecB
b4291: fecA - KpLE2 phage-like element; ferric citrate outer membrane transporter (NCBI), at 4,512,376 to 4,514,700
fecA
b4292: fecR - KpLE2 phage-like element; transmembrane signal transducer for ferric citrate transport (NCBI), at 4,514,787 to 4,515,740
fecR
Position (kb)
4512
4513
4514
4515 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 4511.403 kb on - strand at 4511.405 kb on + strand at 4511.406 kb on - strand at 4511.460 kb on - strand at 4511.523 kb on + strand, within fecB at 4511.523 kb on + strand, within fecB at 4511.526 kb on - strand, within fecB at 4511.565 kb on + strand, within fecB at 4511.570 kb on - strand, within fecB at 4511.572 kb on - strand, within fecB at 4511.595 kb on - strand, within fecB at 4511.595 kb on - strand, within fecB at 4511.604 kb on + strand, within fecB at 4511.604 kb on + strand, within fecB at 4511.604 kb on + strand, within fecB at 4511.607 kb on - strand, within fecB at 4511.607 kb on - strand, within fecB at 4511.616 kb on + strand, within fecB at 4511.622 kb on - strand, within fecB at 4511.638 kb on - strand, within fecB at 4511.638 kb on - strand, within fecB at 4511.639 kb on - strand, within fecB at 4511.639 kb on - strand, within fecB at 4511.683 kb on + strand, within fecB at 4511.683 kb on + strand, within fecB at 4511.727 kb on - strand, within fecB at 4511.727 kb on - strand, within fecB at 4511.745 kb on + strand at 4511.745 kb on + strand, within fecB at 4511.811 kb on + strand, within fecB at 4511.811 kb on + strand, within fecB at 4511.824 kb on + strand, within fecB at 4511.846 kb on - strand, within fecB at 4511.988 kb on + strand, within fecB at 4511.988 kb on + strand, within fecB at 4512.019 kb on - strand, within fecB at 4512.061 kb on - strand, within fecB at 4512.095 kb on + strand, within fecB at 4512.095 kb on + strand, within fecB at 4512.096 kb on - strand, within fecB at 4512.096 kb on - strand, within fecB at 4512.119 kb on - strand, within fecB at 4512.127 kb on + strand, within fecB at 4512.127 kb on + strand, within fecB at 4512.129 kb on - strand, within fecB at 4512.145 kb on + strand, within fecB at 4512.235 kb on + strand, within fecB at 4512.235 kb on + strand, within fecB at 4512.235 kb on + strand, within fecB at 4512.235 kb on + strand, within fecB at 4512.262 kb on - strand at 4512.262 kb on - strand at 4512.262 kb on - strand at 4512.262 kb on - strand at 4512.341 kb on - strand at 4512.372 kb on - strand at 4512.372 kb on - strand at 4512.417 kb on + strand at 4512.417 kb on + strand at 4512.422 kb on + strand at 4512.452 kb on - strand at 4512.460 kb on - strand at 4512.499 kb on - strand at 4512.527 kb on + strand at 4512.527 kb on + strand at 4512.581 kb on - strand at 4512.586 kb on - strand at 4512.673 kb on + strand, within fecA at 4512.735 kb on - strand, within fecA at 4512.735 kb on - strand, within fecA at 4512.771 kb on - strand, within fecA at 4512.812 kb on + strand, within fecA at 4512.820 kb on - strand, within fecA at 4512.830 kb on - strand, within fecA at 4512.835 kb on - strand, within fecA at 4512.835 kb on - strand, within fecA at 4512.881 kb on + strand, within fecA at 4512.902 kb on - strand, within fecA at 4512.902 kb on - strand, within fecA at 4512.962 kb on + strand, within fecA at 4512.962 kb on + strand, within fecA at 4512.967 kb on - strand, within fecA at 4512.986 kb on - strand, within fecA at 4513.025 kb on + strand, within fecA at 4513.025 kb on + strand, within fecA at 4513.037 kb on - strand, within fecA at 4513.042 kb on + strand, within fecA at 4513.045 kb on + strand, within fecA at 4513.045 kb on + strand, within fecA at 4513.050 kb on - strand, within fecA at 4513.050 kb on - strand, within fecA at 4513.050 kb on - strand, within fecA at 4513.050 kb on - strand, within fecA at 4513.050 kb on - strand, within fecA at 4513.052 kb on - strand, within fecA at 4513.134 kb on + strand, within fecA at 4513.174 kb on + strand, within fecA at 4513.182 kb on - strand, within fecA at 4513.271 kb on + strand, within fecA at 4513.389 kb on - strand, within fecA at 4513.437 kb on + strand, within fecA at 4513.445 kb on - strand, within fecA at 4513.568 kb on + strand, within fecA at 4513.598 kb on + strand, within fecA at 4513.598 kb on + strand, within fecA at 4513.606 kb on - strand, within fecA at 4513.708 kb on + strand, within fecA at 4513.723 kb on - strand, within fecA at 4513.780 kb on - strand, within fecA at 4513.792 kb on - strand, within fecA at 4513.792 kb on - strand, within fecA at 4513.808 kb on + strand, within fecA at 4513.821 kb on - strand, within fecA at 4513.821 kb on - strand, within fecA at 4513.847 kb on - strand, within fecA at 4513.854 kb on + strand, within fecA at 4513.901 kb on - strand, within fecA at 4513.934 kb on - strand, within fecA at 4513.961 kb on - strand, within fecA at 4513.991 kb on + strand, within fecA at 4514.038 kb on - strand, within fecA at 4514.038 kb on - strand, within fecA at 4514.078 kb on + strand, within fecA at 4514.097 kb on - strand, within fecA at 4514.110 kb on + strand, within fecA at 4514.281 kb on + strand, within fecA at 4514.281 kb on + strand, within fecA at 4514.304 kb on + strand, within fecA at 4514.304 kb on + strand, within fecA at 4514.427 kb on - strand, within fecA at 4514.427 kb on - strand, within fecA at 4514.468 kb on + strand at 4514.523 kb on - strand at 4514.523 kb on - strand at 4514.544 kb on + strand at 4514.544 kb on + strand at 4514.547 kb on - strand at 4514.549 kb on + strand at 4514.549 kb on + strand at 4514.552 kb on - strand at 4514.556 kb on - strand at 4514.556 kb on - strand at 4514.577 kb on + strand at 4514.581 kb on - strand at 4514.588 kb on + strand at 4514.588 kb on + strand at 4514.588 kb on + strand at 4514.596 kb on - strand at 4514.596 kb on - strand at 4514.598 kb on + strand at 4514.740 kb on - strand at 4514.962 kb on - strand, within fecR at 4515.006 kb on - strand, within fecR at 4515.017 kb on + strand, within fecR at 4515.044 kb on + strand, within fecR at 4515.059 kb on - strand, within fecR at 4515.152 kb on - strand, within fecR at 4515.299 kb on - strand, within fecR at 4515.303 kb on + strand, within fecR at 4515.311 kb on - strand, within fecR at 4515.504 kb on - strand, within fecR at 4515.504 kb on - strand, within fecR at 4515.522 kb on + strand, within fecR at 4515.546 kb on + strand, within fecR at 4515.584 kb on - strand, within fecR at 4515.609 kb on - strand, within fecR at 4515.647 kb on + strand at 4515.669 kb on + strand at 4515.669 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB Aerobic with Ramoplanin 0.000125 mM remove 4,511,403 - -2.6 4,511,405 + -2.5 4,511,406 - -0.8 4,511,460 - +1.4 4,511,523 + fecB b4290 0.10 -0.6 4,511,523 + fecB b4290 0.10 +0.7 4,511,526 - fecB b4290 0.11 +0.5 4,511,565 + fecB b4290 0.15 -0.3 4,511,570 - fecB b4290 0.16 -0.4 4,511,572 - fecB b4290 0.16 +0.6 4,511,595 - fecB b4290 0.18 -1.3 4,511,595 - fecB b4290 0.18 +0.5 4,511,604 + fecB b4290 0.19 -0.8 4,511,604 + fecB b4290 0.19 -0.8 4,511,604 + fecB b4290 0.19 -0.3 4,511,607 - fecB b4290 0.20 -0.1 4,511,607 - fecB b4290 0.20 -0.8 4,511,616 + fecB b4290 0.21 -0.5 4,511,622 - fecB b4290 0.21 -0.4 4,511,638 - fecB b4290 0.23 +2.1 4,511,638 - fecB b4290 0.23 +0.2 4,511,639 - fecB b4290 0.23 +0.0 4,511,639 - fecB b4290 0.23 -0.5 4,511,683 + fecB b4290 0.28 +0.5 4,511,683 + fecB b4290 0.28 -2.5 4,511,727 - fecB b4290 0.33 +0.7 4,511,727 - fecB b4290 0.33 +0.3 4,511,745 + +3.2 4,511,745 + fecB b4290 0.35 -2.4 4,511,811 + fecB b4290 0.42 -0.4 4,511,811 + fecB b4290 0.42 -0.7 4,511,824 + fecB b4290 0.44 -2.6 4,511,846 - fecB b4290 0.46 +0.5 4,511,988 + fecB b4290 0.62 -1.8 4,511,988 + fecB b4290 0.62 -2.4 4,512,019 - fecB b4290 0.65 -0.9 4,512,061 - fecB b4290 0.70 -0.8 4,512,095 + fecB b4290 0.74 -0.0 4,512,095 + fecB b4290 0.74 -1.4 4,512,096 - fecB b4290 0.74 -0.6 4,512,096 - fecB b4290 0.74 +0.3 4,512,119 - fecB b4290 0.76 -2.0 4,512,127 + fecB b4290 0.77 -2.0 4,512,127 + fecB b4290 0.77 -0.0 4,512,129 - fecB b4290 0.78 +0.7 4,512,145 + fecB b4290 0.79 -0.5 4,512,235 + fecB b4290 0.89 +0.4 4,512,235 + fecB b4290 0.89 +1.2 4,512,235 + fecB b4290 0.89 +1.4 4,512,235 + fecB b4290 0.89 +0.3 4,512,262 - -1.4 4,512,262 - -1.0 4,512,262 - +1.0 4,512,262 - -1.1 4,512,341 - +0.1 4,512,372 - -0.0 4,512,372 - -0.9 4,512,417 + -0.3 4,512,417 + -0.7 4,512,422 + -1.0 4,512,452 - -2.0 4,512,460 - +0.3 4,512,499 - -0.7 4,512,527 + -0.3 4,512,527 + +0.4 4,512,581 - +0.7 4,512,586 - -0.9 4,512,673 + fecA b4291 0.13 +0.2 4,512,735 - fecA b4291 0.15 -0.9 4,512,735 - fecA b4291 0.15 +1.5 4,512,771 - fecA b4291 0.17 +0.2 4,512,812 + fecA b4291 0.19 -1.1 4,512,820 - fecA b4291 0.19 +0.3 4,512,830 - fecA b4291 0.20 -0.0 4,512,835 - fecA b4291 0.20 +1.2 4,512,835 - fecA b4291 0.20 +0.9 4,512,881 + fecA b4291 0.22 +0.6 4,512,902 - fecA b4291 0.23 +1.2 4,512,902 - fecA b4291 0.23 +1.1 4,512,962 + fecA b4291 0.25 -1.0 4,512,962 + fecA b4291 0.25 -2.3 4,512,967 - fecA b4291 0.25 -1.0 4,512,986 - fecA b4291 0.26 +0.3 4,513,025 + fecA b4291 0.28 +0.0 4,513,025 + fecA b4291 0.28 +1.1 4,513,037 - fecA b4291 0.28 -1.5 4,513,042 + fecA b4291 0.29 -0.8 4,513,045 + fecA b4291 0.29 -0.2 4,513,045 + fecA b4291 0.29 +0.1 4,513,050 - fecA b4291 0.29 +0.1 4,513,050 - fecA b4291 0.29 -0.6 4,513,050 - fecA b4291 0.29 +0.6 4,513,050 - fecA b4291 0.29 -0.4 4,513,050 - fecA b4291 0.29 +0.2 4,513,052 - fecA b4291 0.29 +0.9 4,513,134 + fecA b4291 0.33 -2.8 4,513,174 + fecA b4291 0.34 -1.4 4,513,182 - fecA b4291 0.35 +0.8 4,513,271 + fecA b4291 0.38 -2.3 4,513,389 - fecA b4291 0.44 +0.3 4,513,437 + fecA b4291 0.46 -0.9 4,513,445 - fecA b4291 0.46 -3.3 4,513,568 + fecA b4291 0.51 +0.2 4,513,598 + fecA b4291 0.53 -0.5 4,513,598 + fecA b4291 0.53 +0.6 4,513,606 - fecA b4291 0.53 +1.2 4,513,708 + fecA b4291 0.57 +0.6 4,513,723 - fecA b4291 0.58 -1.0 4,513,780 - fecA b4291 0.60 +0.6 4,513,792 - fecA b4291 0.61 -1.1 4,513,792 - fecA b4291 0.61 +2.2 4,513,808 + fecA b4291 0.62 +0.1 4,513,821 - fecA b4291 0.62 +1.0 4,513,821 - fecA b4291 0.62 -0.2 4,513,847 - fecA b4291 0.63 -1.7 4,513,854 + fecA b4291 0.64 +1.4 4,513,901 - fecA b4291 0.66 -1.4 4,513,934 - fecA b4291 0.67 -0.4 4,513,961 - fecA b4291 0.68 +0.1 4,513,991 + fecA b4291 0.69 +1.3 4,514,038 - fecA b4291 0.71 -0.1 4,514,038 - fecA b4291 0.71 +1.7 4,514,078 + fecA b4291 0.73 -0.0 4,514,097 - fecA b4291 0.74 -1.1 4,514,110 + fecA b4291 0.75 -0.0 4,514,281 + fecA b4291 0.82 +0.1 4,514,281 + fecA b4291 0.82 +1.6 4,514,304 + fecA b4291 0.83 +0.5 4,514,304 + fecA b4291 0.83 -1.4 4,514,427 - fecA b4291 0.88 -0.6 4,514,427 - fecA b4291 0.88 +0.4 4,514,468 + +0.0 4,514,523 - +0.6 4,514,523 - -1.2 4,514,544 + +0.3 4,514,544 + +1.0 4,514,547 - +0.7 4,514,549 + +0.8 4,514,549 + +0.0 4,514,552 - -1.0 4,514,556 - -2.1 4,514,556 - -0.3 4,514,577 + -0.6 4,514,581 - -0.2 4,514,588 + +0.5 4,514,588 + -0.6 4,514,588 + -0.9 4,514,596 - +0.2 4,514,596 - -1.1 4,514,598 + +0.7 4,514,740 - -0.2 4,514,962 - fecR b4292 0.18 -0.0 4,515,006 - fecR b4292 0.23 -0.5 4,515,017 + fecR b4292 0.24 -0.9 4,515,044 + fecR b4292 0.27 +0.7 4,515,059 - fecR b4292 0.29 +2.2 4,515,152 - fecR b4292 0.38 +1.2 4,515,299 - fecR b4292 0.54 -0.4 4,515,303 + fecR b4292 0.54 +0.3 4,515,311 - fecR b4292 0.55 -1.1 4,515,504 - fecR b4292 0.75 -1.5 4,515,504 - fecR b4292 0.75 +0.3 4,515,522 + fecR b4292 0.77 +0.2 4,515,546 + fecR b4292 0.80 -0.3 4,515,584 - fecR b4292 0.84 +0.6 4,515,609 - fecR b4292 0.86 -1.2 4,515,647 + +0.7 4,515,669 + +0.5 4,515,669 + +2.0
Or see this region's nucleotide sequence