Strain Fitness in Escherichia coli BW25113 around b0742

Experiment: LB Aerobic with Ramoplanin 0.000125 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nttolB and pal are separated by 34 nucleotidespal and ybgF are separated by 9 nucleotidesybgF and lysT are separated by 164 nucleotideslysT and valT are separated by 135 nucleotidesvalT and lysW are separated by 2 nucleotideslysW and valZ are separated by 149 nucleotidesvalZ and lysY are separated by 3 nucleotideslysY and lysZ are separated by 146 nucleotides b0740: tolB - translocation protein TolB precursor (NCBI), at 776,963 to 778,255 tolB b0741: pal - peptidoglycan-associated outer membrane lipoprotein (NCBI), at 778,290 to 778,811 pal b0742: ybgF - hypothetical protein (NCBI), at 778,821 to 779,612 ybgF b0743: lysT - tRNA-Lys (NCBI), at 779,777 to 779,852 lysT b0744: valT - tRNA-Val (NCBI), at 779,988 to 780,063 valT b0745: lysW - tRNA-Lys (NCBI), at 780,066 to 780,141 lysW b0746: valZ - tRNA-Val (NCBI), at 780,291 to 780,366 valZ b0747: lysY - tRNA-Lys (NCBI), at 780,370 to 780,445 lysY b0748: lysZ - tRNA-Lys (NCBI), at 780,592 to 780,667 lysZ Position (kb) 778 779 780Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 777.839 kb on - strand, within tolBat 777.952 kb on - strand, within tolBat 777.954 kb on - strand, within tolBat 777.987 kb on - strand, within tolBat 778.009 kb on - strand, within tolBat 778.014 kb on - strand, within tolBat 778.020 kb on - strand, within tolBat 778.070 kb on + strand, within tolBat 778.127 kb on - strandat 778.150 kb on - strandat 778.150 kb on - strandat 778.212 kb on + strandat 778.212 kb on + strandat 778.212 kb on + strandat 778.220 kb on - strandat 778.222 kb on + strandat 778.316 kb on + strandat 778.316 kb on + strandat 778.382 kb on + strand, within palat 778.395 kb on - strand, within palat 778.420 kb on - strand, within palat 778.422 kb on - strand, within palat 778.455 kb on + strand, within palat 778.455 kb on + strand, within palat 778.560 kb on + strand, within palat 778.568 kb on - strand, within palat 778.596 kb on - strand, within palat 778.600 kb on + strand, within palat 778.604 kb on - strand, within palat 778.656 kb on - strand, within palat 778.656 kb on - strandat 778.661 kb on - strand, within palat 778.713 kb on - strand, within palat 778.752 kb on + strand, within palat 778.752 kb on + strand, within palat 778.752 kb on + strand, within palat 778.780 kb on + strandat 778.780 kb on + strandat 778.805 kb on - strandat 778.851 kb on + strandat 778.856 kb on + strandat 778.864 kb on - strandat 778.870 kb on + strandat 778.932 kb on + strand, within ybgFat 778.940 kb on - strand, within ybgFat 778.940 kb on - strand, within ybgFat 778.940 kb on - strand, within ybgFat 778.940 kb on - strand, within ybgFat 779.036 kb on + strand, within ybgFat 779.145 kb on + strand, within ybgFat 779.145 kb on + strand, within ybgFat 779.158 kb on - strand, within ybgFat 779.196 kb on + strand, within ybgFat 779.201 kb on + strand, within ybgFat 779.201 kb on + strand, within ybgFat 779.204 kb on - strand, within ybgFat 779.263 kb on - strand, within ybgFat 779.263 kb on - strand, within ybgFat 779.269 kb on + strand, within ybgFat 779.342 kb on + strand, within ybgFat 779.342 kb on + strand, within ybgFat 779.392 kb on - strand, within ybgFat 779.428 kb on - strand, within ybgFat 779.510 kb on + strand, within ybgFat 779.544 kb on - strandat 779.544 kb on - strandat 779.565 kb on + strandat 779.610 kb on - strandat 779.619 kb on + strandat 780.222 kb on + strandat 780.222 kb on + strandat 780.232 kb on + strandat 780.232 kb on + strandat 780.242 kb on + strandat 780.245 kb on - strandat 780.274 kb on - strandat 780.357 kb on + strand, within valZat 780.357 kb on + strand, within valZat 780.536 kb on + strandat 780.536 kb on + strandat 780.583 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Aerobic with Ramoplanin 0.000125 mM
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777,839 - tolB b0740 0.68 -1.3
777,952 - tolB b0740 0.76 -1.1
777,954 - tolB b0740 0.77 +0.5
777,987 - tolB b0740 0.79 -1.7
778,009 - tolB b0740 0.81 -1.5
778,014 - tolB b0740 0.81 -1.1
778,020 - tolB b0740 0.82 +1.5
778,070 + tolB b0740 0.86 -1.7
778,127 - +0.5
778,150 - -1.7
778,150 - -0.1
778,212 + +1.5
778,212 + -0.1
778,212 + +0.5
778,220 - -0.1
778,222 + -2.1
778,316 + -0.5
778,316 + -1.3
778,382 + pal b0741 0.18 -0.8
778,395 - pal b0741 0.20 +1.5
778,420 - pal b0741 0.25 -0.1
778,422 - pal b0741 0.25 -0.5
778,455 + pal b0741 0.32 -0.8
778,455 + pal b0741 0.32 -0.8
778,560 + pal b0741 0.52 -1.7
778,568 - pal b0741 0.53 -1.7
778,596 - pal b0741 0.59 -1.5
778,600 + pal b0741 0.59 +0.5
778,604 - pal b0741 0.60 -0.8
778,656 - pal b0741 0.70 +1.5
778,656 - +0.5
778,661 - pal b0741 0.71 -2.6
778,713 - pal b0741 0.81 -1.7
778,752 + pal b0741 0.89 -1.5
778,752 + pal b0741 0.89 -1.1
778,752 + pal b0741 0.89 -1.5
778,780 + -1.8
778,780 + -1.3
778,805 - -2.8
778,851 + -3.3
778,856 + -3.3
778,864 - +1.5
778,870 + -3.0
778,932 + ybgF b0742 0.14 -1.7
778,940 - ybgF b0742 0.15 -3.7
778,940 - ybgF b0742 0.15 -1.9
778,940 - ybgF b0742 0.15 -1.1
778,940 - ybgF b0742 0.15 -1.7
779,036 + ybgF b0742 0.27 -3.8
779,145 + ybgF b0742 0.41 -3.4
779,145 + ybgF b0742 0.41 -2.7
779,158 - ybgF b0742 0.43 -3.3
779,196 + ybgF b0742 0.47 -3.6
779,201 + ybgF b0742 0.48 -1.9
779,201 + ybgF b0742 0.48 -1.3
779,204 - ybgF b0742 0.48 -1.9
779,263 - ybgF b0742 0.56 -0.8
779,263 - ybgF b0742 0.56 -2.8
779,269 + ybgF b0742 0.57 -2.4
779,342 + ybgF b0742 0.66 -2.9
779,342 + ybgF b0742 0.66 -1.3
779,392 - ybgF b0742 0.72 -2.1
779,428 - ybgF b0742 0.77 -2.3
779,510 + ybgF b0742 0.87 -1.9
779,544 - -1.9
779,544 - -4.5
779,565 + -1.8
779,610 - -3.1
779,619 + -0.9
780,222 + +1.4
780,222 + +0.9
780,232 + -0.3
780,232 + +0.0
780,242 + +0.2
780,245 - -2.1
780,274 - +1.5
780,357 + valZ b0746 0.87 -0.0
780,357 + valZ b0746 0.87 -1.1
780,536 + -1.7
780,536 + -0.8
780,583 + -0.9

Or see this region's nucleotide sequence