Experiment: Lactulose (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu5111 and Atu5112 are separated by 202 nucleotides Atu5112 and Atu5113 are separated by 17 nucleotides Atu5113 and Atu5114 are separated by 57 nucleotides Atu5114 and Atu5115 are separated by 3 nucleotides Atu5115 and Atu5116 are separated by 41 nucleotides
Atu5111: Atu5111 - conjugal transfer protein, at 116,187 to 120,815
Atu5111
Atu5112: Atu5112 - hypothetical protein, at 121,018 to 122,340
Atu5112
Atu5113: Atu5113 - transcriptional regulator, at 122,358 to 122,636
Atu5113
Atu5114: Atu5114 - hypothetical protein, at 122,694 to 123,026
Atu5114
Atu5115: Atu5115 - hypothetical protein, at 123,030 to 123,296
Atu5115
Atu5116: Atu5116 - transcriptional regulator protein, at 123,338 to 123,649
Atu5116
Position (kb)
121
122
123 Strain fitness (log2 ratio)
-1
0
1 at 120.197 kb on + strand, within Atu5111 at 120.286 kb on + strand, within Atu5111 at 120.294 kb on - strand, within Atu5111 at 120.435 kb on - strand at 120.593 kb on - strand at 120.593 kb on - strand at 120.600 kb on + strand at 120.622 kb on + strand at 120.622 kb on + strand at 120.622 kb on + strand at 120.622 kb on + strand at 120.622 kb on + strand at 120.622 kb on + strand at 120.630 kb on - strand at 120.630 kb on - strand at 120.630 kb on - strand at 120.630 kb on - strand at 120.630 kb on - strand at 120.647 kb on - strand at 120.677 kb on - strand at 120.712 kb on - strand at 120.712 kb on - strand at 120.749 kb on - strand at 120.749 kb on - strand at 120.749 kb on - strand at 120.757 kb on - strand at 120.769 kb on + strand at 120.769 kb on + strand at 120.769 kb on + strand at 120.778 kb on - strand at 120.793 kb on + strand at 120.824 kb on + strand at 120.827 kb on - strand at 120.829 kb on + strand at 120.832 kb on - strand at 120.832 kb on - strand at 120.832 kb on - strand at 120.933 kb on - strand at 121.407 kb on + strand, within Atu5112 at 121.438 kb on - strand, within Atu5112 at 121.445 kb on + strand, within Atu5112 at 121.584 kb on + strand, within Atu5112 at 121.670 kb on + strand, within Atu5112 at 121.729 kb on - strand, within Atu5112 at 121.997 kb on + strand, within Atu5112 at 122.007 kb on + strand, within Atu5112 at 122.019 kb on + strand, within Atu5112 at 122.253 kb on + strand at 122.275 kb on + strand at 122.351 kb on + strand at 122.734 kb on + strand, within Atu5114 at 122.750 kb on - strand, within Atu5114 at 122.789 kb on - strand, within Atu5114 at 122.799 kb on - strand, within Atu5114 at 122.840 kb on + strand, within Atu5114 at 122.938 kb on - strand, within Atu5114 at 123.043 kb on - strand at 123.052 kb on - strand at 123.068 kb on - strand, within Atu5115 at 123.249 kb on - strand, within Atu5115 at 123.269 kb on + strand, within Atu5115 at 123.277 kb on - strand at 123.308 kb on - strand at 123.319 kb on + strand at 123.329 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Lactulose (C) remove 120,197 + Atu5111 0.87 +0.4 120,286 + Atu5111 0.89 -1.7 120,294 - Atu5111 0.89 +0.7 120,435 - -0.5 120,593 - -0.1 120,593 - -1.3 120,600 + -0.1 120,622 + -0.5 120,622 + -0.4 120,622 + +0.5 120,622 + -0.4 120,622 + -0.2 120,622 + -0.3 120,630 - +0.9 120,630 - -0.2 120,630 - +0.1 120,630 - -0.5 120,630 - -0.3 120,647 - +0.2 120,677 - -0.4 120,712 - -0.3 120,712 - -0.0 120,749 - -0.0 120,749 - -0.0 120,749 - -0.3 120,757 - -0.5 120,769 + +0.5 120,769 + -0.4 120,769 + +0.8 120,778 - +0.4 120,793 + +0.5 120,824 + -0.5 120,827 - -0.2 120,829 + -0.3 120,832 - +0.1 120,832 - -0.4 120,832 - -0.3 120,933 - -0.3 121,407 + Atu5112 0.29 +0.4 121,438 - Atu5112 0.32 +0.1 121,445 + Atu5112 0.32 -0.0 121,584 + Atu5112 0.43 +0.8 121,670 + Atu5112 0.49 +0.4 121,729 - Atu5112 0.54 -0.5 121,997 + Atu5112 0.74 +0.4 122,007 + Atu5112 0.75 -0.0 122,019 + Atu5112 0.76 +0.5 122,253 + -1.5 122,275 + -0.4 122,351 + +0.7 122,734 + Atu5114 0.12 +0.2 122,750 - Atu5114 0.17 +0.7 122,789 - Atu5114 0.29 +1.3 122,799 - Atu5114 0.32 -0.3 122,840 + Atu5114 0.44 +0.1 122,938 - Atu5114 0.73 -0.8 123,043 - -0.4 123,052 - -0.6 123,068 - Atu5115 0.14 -0.1 123,249 - Atu5115 0.82 -0.5 123,269 + Atu5115 0.90 +0.6 123,277 - +0.2 123,308 - +0.6 123,319 + -0.5 123,329 - +0.1
Or see this region's nucleotide sequence