Strain Fitness in Escherichia coli BW25113 around b2744

Experiment: LB Aerobic with Sulfamonomethoxine 0.000125 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntnlpD and pcm are separated by 139 nucleotidespcm and surE overlap by 7 nucleotidessurE and truD overlap by 20 nucleotides b2742: nlpD - predicted outer membrane lipoprotein (NCBI), at 2,865,636 to 2,866,775 nlpD b2743: pcm - protein-L-isoaspartate O-methyltransferase (NCBI), at 2,866,915 to 2,867,541 pcm b2744: surE - acid phosphatase (NCBI), at 2,867,535 to 2,868,296 surE b2745: truD - tRNA pseudouridine synthase D (NCBI), at 2,868,277 to 2,869,326 truD Position (kb) 2867 2868 2869Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 2866.658 kb on + strand, within nlpDat 2866.722 kb on - strandat 2866.836 kb on - strandat 2866.998 kb on + strand, within pcmat 2867.354 kb on + strand, within pcmat 2867.354 kb on + strand, within pcmat 2867.354 kb on + strand, within pcmat 2867.354 kb on + strand, within pcmat 2867.354 kb on + strand, within pcmat 2867.359 kb on - strand, within pcmat 2867.541 kb on - strandat 2867.541 kb on - strandat 2867.616 kb on - strand, within surEat 2867.623 kb on + strand, within surEat 2867.631 kb on + strand, within surEat 2867.672 kb on - strand, within surEat 2867.701 kb on + strand, within surEat 2867.709 kb on - strand, within surEat 2867.747 kb on + strand, within surEat 2867.747 kb on + strand, within surEat 2867.862 kb on - strand, within surEat 2867.862 kb on - strand, within surEat 2867.862 kb on - strand, within surEat 2867.883 kb on + strand, within surEat 2867.883 kb on + strand, within surEat 2868.038 kb on - strand, within surEat 2868.043 kb on - strand, within surEat 2868.043 kb on - strand, within surEat 2868.152 kb on + strand, within surEat 2868.266 kb on - strandat 2868.284 kb on + strandat 2868.332 kb on - strandat 2868.421 kb on - strand, within truDat 2868.647 kb on + strand, within truDat 2868.647 kb on - strand, within truDat 2868.744 kb on + strand, within truDat 2868.779 kb on + strand, within truDat 2868.779 kb on + strand, within truDat 2868.785 kb on - strand, within truDat 2868.785 kb on - strand, within truDat 2868.791 kb on + strand, within truDat 2868.845 kb on - strand, within truDat 2868.845 kb on - strand, within truDat 2868.930 kb on + strand, within truDat 2868.930 kb on + strand, within truDat 2868.986 kb on + strand, within truDat 2868.986 kb on + strand, within truDat 2868.996 kb on - strand, within truDat 2869.037 kb on + strand, within truDat 2869.118 kb on - strand, within truDat 2869.118 kb on - strand, within truDat 2869.194 kb on + strand, within truDat 2869.194 kb on + strand, within truDat 2869.292 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Aerobic with Sulfamonomethoxine 0.000125 mM
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2,866,658 + nlpD b2742 0.90 +0.0
2,866,722 - +0.4
2,866,836 - -0.7
2,866,998 + pcm b2743 0.13 +0.3
2,867,354 + pcm b2743 0.70 -2.8
2,867,354 + pcm b2743 0.70 -1.1
2,867,354 + pcm b2743 0.70 -0.5
2,867,354 + pcm b2743 0.70 +0.6
2,867,354 + pcm b2743 0.70 +0.1
2,867,359 - pcm b2743 0.71 -0.3
2,867,541 - -0.2
2,867,541 - -0.3
2,867,616 - surE b2744 0.11 -1.1
2,867,623 + surE b2744 0.12 -1.5
2,867,631 + surE b2744 0.13 -0.7
2,867,672 - surE b2744 0.18 -1.4
2,867,701 + surE b2744 0.22 +0.4
2,867,709 - surE b2744 0.23 -1.8
2,867,747 + surE b2744 0.28 -2.6
2,867,747 + surE b2744 0.28 +0.8
2,867,862 - surE b2744 0.43 -1.2
2,867,862 - surE b2744 0.43 -0.4
2,867,862 - surE b2744 0.43 -1.3
2,867,883 + surE b2744 0.46 -2.7
2,867,883 + surE b2744 0.46 -2.2
2,868,038 - surE b2744 0.66 -0.8
2,868,043 - surE b2744 0.67 -1.5
2,868,043 - surE b2744 0.67 -1.1
2,868,152 + surE b2744 0.81 -0.3
2,868,266 - -1.6
2,868,284 + -1.9
2,868,332 - +0.3
2,868,421 - truD b2745 0.14 -0.3
2,868,647 + truD b2745 0.35 -0.6
2,868,647 - truD b2745 0.35 +0.1
2,868,744 + truD b2745 0.44 +0.6
2,868,779 + truD b2745 0.48 -0.6
2,868,779 + truD b2745 0.48 -0.4
2,868,785 - truD b2745 0.48 -1.0
2,868,785 - truD b2745 0.48 -0.8
2,868,791 + truD b2745 0.49 -1.0
2,868,845 - truD b2745 0.54 -0.0
2,868,845 - truD b2745 0.54 +0.3
2,868,930 + truD b2745 0.62 -1.1
2,868,930 + truD b2745 0.62 -0.5
2,868,986 + truD b2745 0.68 -0.5
2,868,986 + truD b2745 0.68 -0.2
2,868,996 - truD b2745 0.68 -0.6
2,869,037 + truD b2745 0.72 +2.7
2,869,118 - truD b2745 0.80 -0.2
2,869,118 - truD b2745 0.80 +0.6
2,869,194 + truD b2745 0.87 +0.0
2,869,194 + truD b2745 0.87 -0.6
2,869,292 - +1.1

Or see this region's nucleotide sequence