Strain Fitness in Escherichia coli BW25113 around b2826

Experiment: LB Aerobic with Sulfamonomethoxine 0.00025 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntppdC and ygdB overlap by 16 nucleotidesygdB and ppdB overlap by 4 nucleotidesppdB and ppdA overlap by 10 nucleotidesppdA and thyA are separated by 183 nucleotidesthyA and lgt are separated by 6 nucleotides b2823: ppdC - hypothetical protein (NCBI), at 2,960,463 to 2,960,786 ppdC b2824: ygdB - orf, hypothetical protein (VIMSS), at 2,960,771 to 2,961,178 ygdB b2825: ppdB - hypothetical protein (NCBI), at 2,961,175 to 2,961,738 ppdB b2826: ppdA - hypothetical protein (NCBI), at 2,961,729 to 2,962,199 ppdA b2827: thyA - thymidylate synthase (NCBI), at 2,962,383 to 2,963,177 thyA b2828: lgt - prolipoprotein diacylglyceryl transferase (NCBI), at 2,963,184 to 2,964,059 lgt Position (kb) 2961 2962 2963Strain fitness (log2 ratio) -3 -2 -1 0 1at 2960.736 kb on + strand, within ppdCat 2960.759 kb on - strandat 2960.759 kb on - strandat 2960.974 kb on - strand, within ygdBat 2961.100 kb on + strand, within ygdBat 2961.100 kb on + strand, within ygdBat 2961.102 kb on - strand, within ygdBat 2961.141 kb on + strandat 2961.173 kb on + strandat 2961.343 kb on - strand, within ppdBat 2961.469 kb on + strand, within ppdBat 2961.469 kb on + strand, within ppdBat 2961.483 kb on - strand, within ppdBat 2961.483 kb on - strand, within ppdBat 2961.493 kb on - strand, within ppdBat 2961.493 kb on - strand, within ppdBat 2961.518 kb on + strand, within ppdBat 2961.529 kb on - strand, within ppdBat 2961.529 kb on - strand, within ppdBat 2961.543 kb on - strand, within ppdBat 2961.543 kb on - strand, within ppdBat 2961.543 kb on - strand, within ppdBat 2961.664 kb on + strand, within ppdBat 2961.664 kb on + strand, within ppdBat 2961.794 kb on + strand, within ppdAat 2961.794 kb on + strand, within ppdAat 2961.832 kb on - strand, within ppdAat 2961.832 kb on - strand, within ppdAat 2961.852 kb on - strand, within ppdAat 2961.875 kb on + strand, within ppdAat 2961.875 kb on + strand, within ppdAat 2961.906 kb on + strand, within ppdAat 2961.918 kb on + strand, within ppdAat 2961.943 kb on + strand, within ppdAat 2961.943 kb on + strand, within ppdAat 2961.958 kb on + strand, within ppdAat 2961.958 kb on + strand, within ppdAat 2961.963 kb on + strand, within ppdAat 2962.113 kb on + strand, within ppdAat 2962.122 kb on - strand, within ppdAat 2962.130 kb on + strand, within ppdAat 2962.130 kb on + strand, within ppdAat 2962.149 kb on - strand, within ppdAat 2962.149 kb on - strand, within ppdAat 2962.151 kb on + strand, within ppdAat 2962.173 kb on - strandat 2962.175 kb on + strandat 2962.175 kb on + strandat 2962.175 kb on + strandat 2962.175 kb on + strandat 2962.183 kb on - strandat 2962.215 kb on - strandat 2962.271 kb on + strandat 2962.281 kb on + strandat 2962.281 kb on + strandat 2962.289 kb on - strandat 2962.289 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Aerobic with Sulfamonomethoxine 0.00025 mM
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2,960,736 + ppdC b2823 0.84 -0.5
2,960,759 - -0.6
2,960,759 - +0.9
2,960,974 - ygdB b2824 0.50 -0.7
2,961,100 + ygdB b2824 0.81 -1.0
2,961,100 + ygdB b2824 0.81 +0.4
2,961,102 - ygdB b2824 0.81 +0.7
2,961,141 + -0.4
2,961,173 + +0.1
2,961,343 - ppdB b2825 0.30 +0.1
2,961,469 + ppdB b2825 0.52 +1.0
2,961,469 + ppdB b2825 0.52 +0.4
2,961,483 - ppdB b2825 0.55 -2.3
2,961,483 - ppdB b2825 0.55 -0.4
2,961,493 - ppdB b2825 0.56 -0.2
2,961,493 - ppdB b2825 0.56 +0.3
2,961,518 + ppdB b2825 0.61 +0.3
2,961,529 - ppdB b2825 0.63 +0.2
2,961,529 - ppdB b2825 0.63 -0.3
2,961,543 - ppdB b2825 0.65 +0.0
2,961,543 - ppdB b2825 0.65 +0.1
2,961,543 - ppdB b2825 0.65 +0.6
2,961,664 + ppdB b2825 0.87 +0.8
2,961,664 + ppdB b2825 0.87 -0.1
2,961,794 + ppdA b2826 0.14 -0.6
2,961,794 + ppdA b2826 0.14 -0.4
2,961,832 - ppdA b2826 0.22 +0.1
2,961,832 - ppdA b2826 0.22 -1.0
2,961,852 - ppdA b2826 0.26 -0.9
2,961,875 + ppdA b2826 0.31 -0.5
2,961,875 + ppdA b2826 0.31 -1.0
2,961,906 + ppdA b2826 0.38 -0.5
2,961,918 + ppdA b2826 0.40 -0.5
2,961,943 + ppdA b2826 0.45 -0.0
2,961,943 + ppdA b2826 0.45 +0.3
2,961,958 + ppdA b2826 0.49 -1.3
2,961,958 + ppdA b2826 0.49 -0.7
2,961,963 + ppdA b2826 0.50 +0.9
2,962,113 + ppdA b2826 0.82 -2.0
2,962,122 - ppdA b2826 0.83 -0.5
2,962,130 + ppdA b2826 0.85 -1.0
2,962,130 + ppdA b2826 0.85 -2.4
2,962,149 - ppdA b2826 0.89 +1.7
2,962,149 - ppdA b2826 0.89 +1.3
2,962,151 + ppdA b2826 0.90 -2.2
2,962,173 - +0.1
2,962,175 + -3.3
2,962,175 + -1.6
2,962,175 + -2.1
2,962,175 + -1.4
2,962,183 - -0.3
2,962,215 - -0.4
2,962,271 + -2.4
2,962,281 + -1.9
2,962,281 + -2.4
2,962,289 - -0.1
2,962,289 - +0.0

Or see this region's nucleotide sequence