Strain Fitness in Escherichia coli BW25113 around b2836

Experiment: LB Aerobic with Spiramycin 0.0000625 mM

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	LB Aerobic with Spiramycin 0.0000625 mM
2,970,900	-	ygeD	b2835	0.18	+0.8
2,970,970	-	ygeD	b2835	0.23	+0.2
2,971,028	+	ygeD	b2835	0.28	+0.3
2,971,028	+	ygeD	b2835	0.28	+0.3
2,971,031	-	ygeD	b2835	0.28	+0.2
2,971,031	-	ygeD	b2835	0.28	+0.0
2,971,033	-	ygeD	b2835	0.29	+0.6
2,971,068	-	ygeD	b2835	0.32	-0.7
2,971,154	-	ygeD	b2835	0.39	-0.4
2,971,180	+	ygeD	b2835	0.41	+0.4
2,971,303	-	ygeD	b2835	0.51	-0.5
2,971,305	+	ygeD	b2835	0.51	+0.1
2,971,360	+	ygeD	b2835	0.56	+0.7
2,971,360	+	ygeD	b2835	0.56	-0.8
2,971,438	-	ygeD	b2835	0.63	+0.3
2,971,489	+	ygeD	b2835	0.67	+0.2
2,971,489	+	ygeD	b2835	0.67	+0.8
2,971,489	+	ygeD	b2835	0.67	+0.4
2,971,571	-	ygeD	b2835	0.74	+0.3
2,971,581	+	ygeD	b2835	0.75	+1.0
2,971,617	+	ygeD	b2835	0.78	+0.1
2,971,625	-	ygeD	b2835	0.78	-0.6
2,971,625	-	ygeD	b2835	0.78	+0.8
2,971,634	+	ygeD	b2835	0.79	-0.2
2,971,656	-	ygeD	b2835	0.81	+0.1
2,971,656	-	ygeD	b2835	0.81	-1.2
2,971,737	-	ygeD	b2835	0.88	+0.4
2,971,737	-	ygeD	b2835	0.88	+0.4
2,971,753	-	ygeD	b2835	0.89	+0.5
2,971,776	-				+1.1
2,971,857	+				+0.4
2,971,864	+				+0.2
2,971,869	+				-0.1
2,971,869	+				-0.9
2,971,970	+				+0.3
2,972,106	-	aas	b2836	0.11	+0.5
2,972,202	-	aas	b2836	0.15	+0.4
2,972,210	+	aas	b2836	0.15	-0.1
2,972,215	+	aas	b2836	0.16	+1.0
2,972,215	+	aas	b2836	0.16	+1.4
2,972,239	+	aas	b2836	0.17	-1.8
2,972,261	-	aas	b2836	0.18	+0.1
2,972,261	-	aas	b2836	0.18	+1.0
2,972,467	+	aas	b2836	0.27	+0.3
2,972,498	-	aas	b2836	0.29	+0.3
2,972,589	-	aas	b2836	0.33	+0.4
2,972,629	+	aas	b2836	0.35	+0.3
2,972,660	-	aas	b2836	0.36	+0.4
2,972,660	-	aas	b2836	0.36	+0.9
2,972,667	+	aas	b2836	0.37	+0.6
2,972,668	-	aas	b2836	0.37	+0.8
2,972,750	-	aas	b2836	0.40	+0.6
2,972,750	-	aas	b2836	0.40	+0.9
2,972,752	-	aas	b2836	0.41	-0.1
2,972,752	-	aas	b2836	0.41	+0.8
2,972,776	+	aas	b2836	0.42	+1.1
2,972,789	+	aas	b2836	0.42	+1.0
2,972,887	-	aas	b2836	0.47	+0.8
2,972,905	+	aas	b2836	0.48	+1.8
2,972,952	+	aas	b2836	0.50	+0.5
2,973,147	+	aas	b2836	0.59	+0.7
2,973,200	-	aas	b2836	0.61	+1.4
2,973,253	+	aas	b2836	0.64	+1.0
2,973,261	-	aas	b2836	0.64	+0.7
2,973,261	-	aas	b2836	0.64	-0.4
2,973,351	+	aas	b2836	0.68	+1.0
2,973,351	+	aas	b2836	0.68	+0.1
2,973,354	-	aas	b2836	0.68	+0.2
2,973,354	-	aas	b2836	0.68	+0.2
2,973,526	-	aas	b2836	0.76	+1.2
2,973,540	-	aas	b2836	0.77	-0.0
2,973,699	-	aas	b2836	0.84	+0.7
2,973,741	+	aas	b2836	0.86	-0.3
2,973,754	+	aas	b2836	0.87	+0.9
2,973,831	-				+0.6
2,973,831	-				+0.2
2,973,842	-				+0.5
2,973,894	-				+0.0
2,973,916	+				+0.5
2,973,918	+				+0.6
2,973,974	-				+0.3
2,973,995	+				+0.6
2,974,053	-				-0.2
2,974,055	+				+1.8
2,974,055	+				+1.2
2,974,090	+				+1.2
2,974,090	+				+2.0
2,974,097	-				+0.4
2,974,148	+	rygA	b4444	0.27	-0.1
2,974,161	-	rygA	b4444	0.42	+0.2
2,974,163	-	rygA	b4444	0.44	+0.9
2,974,163	-	rygA	b4444	0.44	-0.6
2,974,168	-	rygA	b4444	0.50	+0.3
2,974,174	-	rygA	b4444	0.57	+0.0
2,974,183	-	rygA	b4444	0.67	-0.4
2,974,340	+	omrB	b4445	0.17	+0.1
2,974,353	-	omrB	b4445	0.33	-0.4
2,974,363	-	omrB	b4445	0.45	-0.3
2,974,381	-	omrB	b4445	0.67	+0.5
2,974,429	-				+0.4
2,974,581	-				+0.5
2,974,635	+				-0.4
2,974,635	+				+0.0
2,974,636	-				+0.6
2,974,636	-				-0.5
2,974,684	+				+0.3
2,974,684	+				-0.2
2,974,723	+				+0.3
2,974,733	+	galR	b2837	0.11	-0.8
2,974,733	+	galR	b2837	0.11	+0.0
2,974,741	-	galR	b2837	0.12	-1.1
2,974,741	-	galR	b2837	0.12	+0.1
2,974,751	+	galR	b2837	0.13	-1.1
2,974,793	+	galR	b2837	0.17	-0.1
2,974,793	+	galR	b2837	0.17	-0.0