Strain Fitness in Escherichia coli BW25113 around b4398

Experiment: LB Aerobic with Spiramycin 0.000125 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntrob and creA are separated by 210 nucleotidescreA and creB are separated by 12 nucleotidescreB and creC overlap by 1 nucleotides b4396: rob - DNA-binding transcriptional activator (NCBI), at 4,632,464 to 4,633,333 rob b4397: creA - hypothetical protein (NCBI), at 4,633,544 to 4,634,017 creA b4398: creB - DNA-binding response regulator in two-component regulatory system with CreC (NCBI), at 4,634,030 to 4,634,719 creB b4399: creC - sensory histidine kinase in two-component regulatory system with CreB or PhoB, regulator of the CreBC regulon (NCBI), at 4,634,719 to 4,636,143 creC Position (kb) 4634 4635Strain fitness (log2 ratio) -2 -1 0 1 2at 4633.037 kb on + strand, within robat 4633.037 kb on + strand, within robat 4633.038 kb on - strand, within robat 4633.089 kb on + strand, within robat 4633.089 kb on + strand, within robat 4633.102 kb on - strand, within robat 4633.102 kb on - strand, within robat 4633.193 kb on + strand, within robat 4633.193 kb on + strand, within robat 4633.193 kb on + strand, within robat 4633.227 kb on - strand, within robat 4633.227 kb on - strand, within robat 4633.244 kb on - strand, within robat 4633.274 kb on - strandat 4633.306 kb on - strandat 4633.317 kb on + strandat 4633.364 kb on - strandat 4633.367 kb on + strandat 4633.441 kb on - strandat 4633.445 kb on - strandat 4633.540 kb on - strandat 4633.602 kb on + strand, within creAat 4633.610 kb on - strand, within creAat 4633.621 kb on + strand, within creAat 4633.803 kb on + strand, within creAat 4633.871 kb on + strand, within creAat 4633.885 kb on + strand, within creAat 4633.888 kb on + strand, within creAat 4633.897 kb on - strand, within creAat 4633.942 kb on - strand, within creAat 4633.942 kb on - strand, within creAat 4634.001 kb on + strandat 4634.003 kb on + strandat 4634.232 kb on + strand, within creBat 4634.256 kb on + strand, within creBat 4634.274 kb on - strand, within creBat 4634.276 kb on + strand, within creBat 4634.318 kb on + strand, within creBat 4634.364 kb on + strand, within creBat 4634.376 kb on + strand, within creBat 4634.376 kb on + strand, within creBat 4634.381 kb on + strand, within creBat 4634.425 kb on - strand, within creBat 4634.446 kb on + strand, within creBat 4634.454 kb on - strand, within creBat 4634.506 kb on + strand, within creBat 4634.553 kb on - strand, within creBat 4634.588 kb on + strand, within creBat 4634.612 kb on + strand, within creBat 4634.637 kb on + strand, within creBat 4634.645 kb on - strand, within creBat 4634.674 kb on + strandat 4634.676 kb on + strandat 4634.676 kb on + strandat 4634.704 kb on + strandat 4634.711 kb on + strandat 4634.714 kb on + strandat 4634.716 kb on + strandat 4634.721 kb on - strandat 4634.721 kb on - strandat 4634.887 kb on - strand, within creCat 4634.894 kb on + strand, within creCat 4634.935 kb on - strand, within creCat 4634.935 kb on - strand, within creCat 4634.943 kb on + strand, within creCat 4634.943 kb on + strand, within creCat 4634.946 kb on - strand, within creCat 4635.008 kb on - strand, within creCat 4635.036 kb on - strand, within creCat 4635.036 kb on - strand, within creCat 4635.089 kb on + strand, within creCat 4635.089 kb on + strand, within creCat 4635.162 kb on + strand, within creCat 4635.178 kb on + strand, within creCat 4635.178 kb on + strand, within creCat 4635.186 kb on - strand, within creCat 4635.198 kb on + strand, within creCat 4635.327 kb on + strand, within creCat 4635.334 kb on + strand, within creCat 4635.344 kb on + strand, within creCat 4635.392 kb on - strand, within creCat 4635.392 kb on - strand, within creCat 4635.416 kb on + strand, within creCat 4635.434 kb on - strand, within creCat 4635.450 kb on + strand, within creCat 4635.667 kb on - strand, within creCat 4635.667 kb on - strand, within creC

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Aerobic with Spiramycin 0.000125 mM
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4,633,037 + rob b4396 0.66 -0.6
4,633,037 + rob b4396 0.66 -0.5
4,633,038 - rob b4396 0.66 -1.0
4,633,089 + rob b4396 0.72 -2.5
4,633,089 + rob b4396 0.72 -0.6
4,633,102 - rob b4396 0.73 -0.0
4,633,102 - rob b4396 0.73 -0.0
4,633,193 + rob b4396 0.84 -1.3
4,633,193 + rob b4396 0.84 +0.4
4,633,193 + rob b4396 0.84 -2.6
4,633,227 - rob b4396 0.88 -1.7
4,633,227 - rob b4396 0.88 -0.3
4,633,244 - rob b4396 0.90 -0.3
4,633,274 - -0.0
4,633,306 - -0.9
4,633,317 + -0.6
4,633,364 - +0.3
4,633,367 + -0.2
4,633,441 - +0.4
4,633,445 - -0.7
4,633,540 - +0.7
4,633,602 + creA b4397 0.12 +0.0
4,633,610 - creA b4397 0.14 -0.1
4,633,621 + creA b4397 0.16 +0.6
4,633,803 + creA b4397 0.55 +0.2
4,633,871 + creA b4397 0.69 -0.6
4,633,885 + creA b4397 0.72 +0.5
4,633,888 + creA b4397 0.73 -0.0
4,633,897 - creA b4397 0.74 -0.2
4,633,942 - creA b4397 0.84 +1.5
4,633,942 - creA b4397 0.84 +0.4
4,634,001 + -0.5
4,634,003 + -1.4
4,634,232 + creB b4398 0.29 +0.4
4,634,256 + creB b4398 0.33 +0.9
4,634,274 - creB b4398 0.35 +0.5
4,634,276 + creB b4398 0.36 +0.1
4,634,318 + creB b4398 0.42 +0.1
4,634,364 + creB b4398 0.48 +0.3
4,634,376 + creB b4398 0.50 +1.0
4,634,376 + creB b4398 0.50 +0.6
4,634,381 + creB b4398 0.51 +1.1
4,634,425 - creB b4398 0.57 -0.2
4,634,446 + creB b4398 0.60 +1.0
4,634,454 - creB b4398 0.61 +0.8
4,634,506 + creB b4398 0.69 +0.1
4,634,553 - creB b4398 0.76 -0.1
4,634,588 + creB b4398 0.81 +0.3
4,634,612 + creB b4398 0.84 -0.0
4,634,637 + creB b4398 0.88 -0.4
4,634,645 - creB b4398 0.89 -0.2
4,634,674 + +0.2
4,634,676 + +0.7
4,634,676 + +0.7
4,634,704 + +0.3
4,634,711 + -0.8
4,634,714 + -0.4
4,634,716 + +0.2
4,634,721 - +1.4
4,634,721 - +1.2
4,634,887 - creC b4399 0.12 +0.4
4,634,894 + creC b4399 0.12 -0.7
4,634,935 - creC b4399 0.15 -0.6
4,634,935 - creC b4399 0.15 -0.4
4,634,943 + creC b4399 0.16 -0.7
4,634,943 + creC b4399 0.16 -1.6
4,634,946 - creC b4399 0.16 -0.2
4,635,008 - creC b4399 0.20 -0.2
4,635,036 - creC b4399 0.22 +0.3
4,635,036 - creC b4399 0.22 +0.2
4,635,089 + creC b4399 0.26 -0.3
4,635,089 + creC b4399 0.26 +0.4
4,635,162 + creC b4399 0.31 +0.1
4,635,178 + creC b4399 0.32 -0.5
4,635,178 + creC b4399 0.32 +0.6
4,635,186 - creC b4399 0.33 -1.1
4,635,198 + creC b4399 0.34 +0.1
4,635,327 + creC b4399 0.43 +0.7
4,635,334 + creC b4399 0.43 +0.4
4,635,344 + creC b4399 0.44 -0.2
4,635,392 - creC b4399 0.47 -1.4
4,635,392 - creC b4399 0.47 +0.8
4,635,416 + creC b4399 0.49 -0.6
4,635,434 - creC b4399 0.50 +1.9
4,635,450 + creC b4399 0.51 -0.6
4,635,667 - creC b4399 0.67 -1.0
4,635,667 - creC b4399 0.67 -0.4

Or see this region's nucleotide sequence