Strain Fitness in Escherichia coli BW25113 around b2990

Experiment: LB Aerobic with Spiramycin 0.000125 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntb2989 and hybG are separated by 122 nucleotideshybG and hybF are separated by 12 nucleotideshybF and hybE overlap by 8 nucleotideshybE and hybD overlap by 8 nucleotides b2989: b2989 - orf, hypothetical protein (VIMSS), at 3,136,749 to 3,137,615 b2989 b2990: hybG - hydrogenase 2 accessory protein (NCBI), at 3,137,738 to 3,137,986 hybG b2991: hybF - protein involved with the maturation of hydrogenases 1 and 2 (NCBI), at 3,137,999 to 3,138,340 hybF b2992: hybE - hydrogenase 2-specific chaperone (NCBI), at 3,138,333 to 3,138,821 hybE b2993: hybD - predicted maturation element for hydrogenase 2 (NCBI), at 3,138,814 to 3,139,308 hybD Position (kb) 3137 3138Strain fitness (log2 ratio) -1 0 1at 3137.122 kb on - strand, within b2989at 3137.208 kb on + strand, within b2989at 3137.208 kb on + strand, within b2989at 3137.294 kb on + strand, within b2989at 3137.339 kb on - strand, within b2989at 3137.360 kb on + strandat 3137.372 kb on + strand, within b2989at 3137.372 kb on + strand, within b2989at 3137.406 kb on + strand, within b2989at 3137.414 kb on - strand, within b2989at 3137.463 kb on + strand, within b2989at 3137.525 kb on - strand, within b2989at 3137.528 kb on + strand, within b2989at 3137.615 kb on - strandat 3137.615 kb on - strandat 3137.691 kb on - strandat 3137.700 kb on + strandat 3137.714 kb on + strandat 3137.717 kb on - strandat 3137.839 kb on - strand, within hybGat 3137.839 kb on - strand, within hybGat 3137.907 kb on + strand, within hybGat 3137.986 kb on + strandat 3137.986 kb on + strandat 3138.086 kb on + strand, within hybFat 3138.146 kb on - strand, within hybFat 3138.152 kb on + strand, within hybFat 3138.166 kb on + strand, within hybFat 3138.233 kb on - strand, within hybFat 3138.274 kb on - strand, within hybFat 3138.274 kb on - strand, within hybFat 3138.363 kb on + strandat 3138.474 kb on + strand, within hybEat 3138.474 kb on + strand, within hybEat 3138.640 kb on + strand, within hybEat 3138.640 kb on + strand, within hybEat 3138.649 kb on + strand, within hybEat 3138.658 kb on - strand, within hybEat 3138.707 kb on + strand, within hybEat 3138.715 kb on - strand, within hybEat 3138.839 kb on - strandat 3138.839 kb on - strandat 3138.928 kb on + strand, within hybD

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Aerobic with Spiramycin 0.000125 mM
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3,137,122 - b2989 b2989 0.43 -0.7
3,137,208 + b2989 b2989 0.53 -0.6
3,137,208 + b2989 b2989 0.53 +0.5
3,137,294 + b2989 b2989 0.63 -1.5
3,137,339 - b2989 b2989 0.68 +1.0
3,137,360 + +1.4
3,137,372 + b2989 b2989 0.72 -0.1
3,137,372 + b2989 b2989 0.72 -1.2
3,137,406 + b2989 b2989 0.76 +0.3
3,137,414 - b2989 b2989 0.77 -0.6
3,137,463 + b2989 b2989 0.82 -0.0
3,137,525 - b2989 b2989 0.90 -0.7
3,137,528 + b2989 b2989 0.90 -1.7
3,137,615 - -0.2
3,137,615 - +0.1
3,137,691 - -0.3
3,137,700 + -0.2
3,137,714 + -1.4
3,137,717 - -0.5
3,137,839 - hybG b2990 0.41 +0.6
3,137,839 - hybG b2990 0.41 +0.6
3,137,907 + hybG b2990 0.68 +1.2
3,137,986 + +0.8
3,137,986 + +0.9
3,138,086 + hybF b2991 0.25 +0.1
3,138,146 - hybF b2991 0.43 -0.1
3,138,152 + hybF b2991 0.45 +0.5
3,138,166 + hybF b2991 0.49 -0.3
3,138,233 - hybF b2991 0.68 +0.4
3,138,274 - hybF b2991 0.80 -0.0
3,138,274 - hybF b2991 0.80 -0.5
3,138,363 + -0.1
3,138,474 + hybE b2992 0.29 +0.1
3,138,474 + hybE b2992 0.29 +0.1
3,138,640 + hybE b2992 0.63 -0.3
3,138,640 + hybE b2992 0.63 -0.2
3,138,649 + hybE b2992 0.65 -0.5
3,138,658 - hybE b2992 0.66 -1.1
3,138,707 + hybE b2992 0.76 -0.4
3,138,715 - hybE b2992 0.78 +0.0
3,138,839 - -0.9
3,138,839 - -0.0
3,138,928 + hybD b2993 0.23 +0.8

Or see this region's nucleotide sequence