Experiment: LB Aerobic with Spiramycin 0.000125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt gmd and wcaF are separated by 25 nucleotides wcaF and wcaE are separated by 15 nucleotides wcaE and wcaD are separated by 10 nucleotides
b2053: gmd - GDP-D-mannose dehydratase, NAD(P)-binding (NCBI), at 2,125,217 to 2,126,338
gmd
b2054: wcaF - predicted acyl transferase (NCBI), at 2,126,364 to 2,126,912
wcaF
b2055: wcaE - predicted glycosyl transferase (NCBI), at 2,126,928 to 2,127,674
wcaE
b2056: wcaD - predicted colanic acid polymerase (NCBI), at 2,127,685 to 2,128,902
wcaD
Position (kb)
2126
2127 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2125.556 kb on + strand, within gmd at 2125.566 kb on + strand, within gmd at 2125.570 kb on + strand, within gmd at 2125.570 kb on + strand, within gmd at 2125.643 kb on + strand, within gmd at 2125.706 kb on + strand, within gmd at 2125.707 kb on - strand, within gmd at 2125.797 kb on + strand, within gmd at 2125.797 kb on + strand, within gmd at 2125.828 kb on + strand, within gmd at 2125.887 kb on + strand, within gmd at 2125.941 kb on - strand, within gmd at 2126.048 kb on + strand, within gmd at 2126.090 kb on - strand, within gmd at 2126.093 kb on - strand, within gmd at 2126.151 kb on + strand, within gmd at 2126.181 kb on - strand, within gmd at 2126.225 kb on - strand, within gmd at 2126.225 kb on - strand, within gmd at 2126.247 kb on - strand at 2126.247 kb on - strand at 2126.247 kb on - strand at 2126.267 kb on + strand at 2126.388 kb on + strand at 2126.398 kb on - strand at 2126.451 kb on - strand at 2126.451 kb on - strand, within wcaF at 2126.456 kb on - strand, within wcaF at 2126.456 kb on - strand, within wcaF at 2126.498 kb on + strand, within wcaF at 2126.515 kb on + strand, within wcaF at 2126.550 kb on - strand, within wcaF at 2126.550 kb on - strand, within wcaF at 2126.562 kb on + strand, within wcaF at 2126.562 kb on - strand, within wcaF at 2126.562 kb on - strand, within wcaF at 2126.577 kb on + strand, within wcaF at 2126.577 kb on + strand, within wcaF at 2126.584 kb on - strand, within wcaF at 2126.587 kb on + strand, within wcaF at 2126.614 kb on + strand, within wcaF at 2126.616 kb on + strand, within wcaF at 2126.621 kb on + strand, within wcaF at 2126.629 kb on + strand, within wcaF at 2126.660 kb on - strand, within wcaF at 2126.681 kb on + strand, within wcaF at 2126.681 kb on + strand, within wcaF at 2126.914 kb on + strand at 2127.243 kb on - strand, within wcaE at 2127.467 kb on - strand, within wcaE at 2127.583 kb on + strand, within wcaE at 2127.584 kb on + strand, within wcaE at 2127.617 kb on + strand at 2127.663 kb on + strand at 2127.663 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB Aerobic with Spiramycin 0.000125 mM remove 2,125,556 + gmd b2053 0.30 -1.3 2,125,566 + gmd b2053 0.31 -0.3 2,125,570 + gmd b2053 0.31 -0.3 2,125,570 + gmd b2053 0.31 -0.2 2,125,643 + gmd b2053 0.38 -0.3 2,125,706 + gmd b2053 0.44 +0.6 2,125,707 - gmd b2053 0.44 +1.3 2,125,797 + gmd b2053 0.52 -1.5 2,125,797 + gmd b2053 0.52 +0.3 2,125,828 + gmd b2053 0.54 -0.9 2,125,887 + gmd b2053 0.60 +0.9 2,125,941 - gmd b2053 0.65 -0.4 2,126,048 + gmd b2053 0.74 +0.8 2,126,090 - gmd b2053 0.78 -0.5 2,126,093 - gmd b2053 0.78 -0.6 2,126,151 + gmd b2053 0.83 +0.7 2,126,181 - gmd b2053 0.86 -0.8 2,126,225 - gmd b2053 0.90 -0.5 2,126,225 - gmd b2053 0.90 +0.3 2,126,247 - +0.3 2,126,247 - -0.1 2,126,247 - -1.1 2,126,267 + -0.5 2,126,388 + -0.5 2,126,398 - +0.3 2,126,451 - +0.5 2,126,451 - wcaF b2054 0.16 -0.3 2,126,456 - wcaF b2054 0.17 -0.3 2,126,456 - wcaF b2054 0.17 -1.1 2,126,498 + wcaF b2054 0.24 -0.8 2,126,515 + wcaF b2054 0.28 -0.7 2,126,550 - wcaF b2054 0.34 -2.1 2,126,550 - wcaF b2054 0.34 +1.8 2,126,562 + wcaF b2054 0.36 -1.8 2,126,562 - wcaF b2054 0.36 -0.6 2,126,562 - wcaF b2054 0.36 +0.2 2,126,577 + wcaF b2054 0.39 -1.1 2,126,577 + wcaF b2054 0.39 -0.1 2,126,584 - wcaF b2054 0.40 +1.5 2,126,587 + wcaF b2054 0.41 -2.0 2,126,614 + wcaF b2054 0.46 +0.5 2,126,616 + wcaF b2054 0.46 +0.2 2,126,621 + wcaF b2054 0.47 +0.0 2,126,629 + wcaF b2054 0.48 -1.3 2,126,660 - wcaF b2054 0.54 +1.5 2,126,681 + wcaF b2054 0.58 +0.5 2,126,681 + wcaF b2054 0.58 +1.5 2,126,914 + +1.5 2,127,243 - wcaE b2055 0.42 +1.5 2,127,467 - wcaE b2055 0.72 +1.5 2,127,583 + wcaE b2055 0.88 +1.5 2,127,584 + wcaE b2055 0.88 +1.5 2,127,617 + +1.5 2,127,663 + +1.5 2,127,663 + +1.5
Or see this region's nucleotide sequence