Strain Fitness in Escherichia coli BW25113 around b1729

Experiment: LB Aerobic with Spiramycin 0.000125 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyniC and ydjM are separated by 162 nucleotidesydjM and ydjN are separated by 132 nucleotidesydjN and ydjO are separated by 3 nucleotides b1727: yniC - predicted hydrolase (NCBI), at 1,807,404 to 1,808,072 yniC b1728: ydjM - predicted inner membrane protein regulated by LexA (RefSeq), at 1,808,235 to 1,808,825 ydjM b1729: ydjN - predicted transporter (NCBI), at 1,808,958 to 1,810,349 ydjN b1730: ydjO - predicted protein (RefSeq), at 1,810,353 to 1,811,156 ydjO Position (kb) 1808 1809 1810 1811Strain fitness (log2 ratio) -2 -1 0 1at 1808.090 kb on + strandat 1808.185 kb on - strandat 1808.203 kb on - strandat 1808.359 kb on + strand, within ydjMat 1808.524 kb on - strand, within ydjMat 1808.524 kb on - strand, within ydjMat 1808.679 kb on + strand, within ydjMat 1808.870 kb on + strandat 1808.924 kb on - strandat 1809.193 kb on + strand, within ydjNat 1809.214 kb on + strand, within ydjNat 1809.242 kb on + strand, within ydjNat 1809.242 kb on + strand, within ydjNat 1809.720 kb on + strand, within ydjNat 1809.720 kb on + strand, within ydjNat 1810.253 kb on + strandat 1810.253 kb on + strandat 1810.284 kb on + strandat 1810.284 kb on + strandat 1810.343 kb on + strandat 1810.445 kb on - strand, within ydjOat 1810.493 kb on + strand, within ydjOat 1810.515 kb on + strand, within ydjOat 1811.304 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Aerobic with Spiramycin 0.000125 mM
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1,808,090 + +1.5
1,808,185 - -0.7
1,808,203 - +0.0
1,808,359 + ydjM b1728 0.21 +0.8
1,808,524 - ydjM b1728 0.49 +1.0
1,808,524 - ydjM b1728 0.49 +0.1
1,808,679 + ydjM b1728 0.75 -0.3
1,808,870 + +0.6
1,808,924 - +0.5
1,809,193 + ydjN b1729 0.17 +0.4
1,809,214 + ydjN b1729 0.18 +1.0
1,809,242 + ydjN b1729 0.20 -2.2
1,809,242 + ydjN b1729 0.20 +0.4
1,809,720 + ydjN b1729 0.55 +0.3
1,809,720 + ydjN b1729 0.55 +0.8
1,810,253 + +0.7
1,810,253 + +0.1
1,810,284 + -0.4
1,810,284 + +0.9
1,810,343 + +0.5
1,810,445 - ydjO b1730 0.11 +1.4
1,810,493 + ydjO b1730 0.17 -1.2
1,810,515 + ydjO b1730 0.20 +1.4
1,811,304 - +1.4

Or see this region's nucleotide sequence