Strain Fitness in Escherichia coli BW25113 around b1629

Experiment: LB Aerobic with Spiramycin 0.000125 mM

Add experiment(s):

Zoom: Pan:

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

download SVG
500 ntrsxA and rsxB overlap by 1 nucleotidesrsxB and rsxC overlap by 8 nucleotidesrsxC and rsxD are separated by 0 nucleotides b1627: rsxA - Na(+)-translocating NADH-quinone reductase subunit E (NCBI), at 1,703,791 to 1,704,372 rsxA b1628: rsxB - electron transport complex protein RnfB (NCBI), at 1,704,372 to 1,704,950 rsxB b1629: rsxC - electron transport complex protein RnfC (NCBI), at 1,704,943 to 1,707,165 rsxC b1630: rsxD - electron transport complex protein RnfD (NCBI), at 1,707,166 to 1,708,224 rsxD Position (kb) 1704 1705 1706 1707 1708Strain fitness (log2 ratio) -2 -1 0 1 2 3at 1704.048 kb on + strand, within rsxAat 1704.090 kb on - strand, within rsxAat 1704.235 kb on - strand, within rsxAat 1704.376 kb on + strandat 1704.376 kb on + strandat 1704.377 kb on - strandat 1704.525 kb on - strand, within rsxBat 1704.772 kb on + strand, within rsxBat 1704.806 kb on + strand, within rsxBat 1705.039 kb on + strandat 1705.042 kb on - strandat 1705.235 kb on - strand, within rsxCat 1705.290 kb on + strand, within rsxCat 1705.290 kb on + strand, within rsxCat 1705.308 kb on - strand, within rsxCat 1705.308 kb on - strand, within rsxCat 1705.459 kb on + strand, within rsxCat 1705.459 kb on + strand, within rsxCat 1705.529 kb on - strand, within rsxCat 1705.579 kb on + strand, within rsxCat 1705.727 kb on - strand, within rsxCat 1705.812 kb on + strand, within rsxCat 1705.999 kb on + strand, within rsxCat 1706.363 kb on + strand, within rsxCat 1706.547 kb on - strand, within rsxCat 1706.829 kb on + strand, within rsxCat 1706.859 kb on + strand, within rsxCat 1706.859 kb on + strand, within rsxCat 1706.883 kb on - strand, within rsxCat 1706.883 kb on - strand, within rsxCat 1706.924 kb on + strand, within rsxCat 1707.252 kb on + strandat 1707.252 kb on + strandat 1707.328 kb on + strand, within rsxDat 1707.414 kb on + strand, within rsxDat 1707.414 kb on + strand, within rsxDat 1707.473 kb on - strand, within rsxDat 1707.579 kb on - strand, within rsxDat 1707.696 kb on - strand, within rsxDat 1707.716 kb on + strand, within rsxDat 1707.793 kb on + strand, within rsxDat 1707.803 kb on - strand, within rsxDat 1707.895 kb on + strand, within rsxDat 1707.914 kb on - strand, within rsxDat 1707.924 kb on + strand, within rsxDat 1708.021 kb on + strand, within rsxDat 1708.161 kb on + strandat 1708.161 kb on + strand

download strain data

Per-strain Table

Position Strand Gene LocusTag Fraction LB Aerobic with Spiramycin 0.000125 mM
remove
1,704,048 + rsxA b1627 0.44 +1.8
1,704,090 - rsxA b1627 0.51 +1.1
1,704,235 - rsxA b1627 0.76 +1.9
1,704,376 + +1.2
1,704,376 + +0.7
1,704,377 - -0.6
1,704,525 - rsxB b1628 0.26 -1.2
1,704,772 + rsxB b1628 0.69 +1.1
1,704,806 + rsxB b1628 0.75 +0.6
1,705,039 + +0.5
1,705,042 - +2.4
1,705,235 - rsxC b1629 0.13 -1.4
1,705,290 + rsxC b1629 0.16 +1.4
1,705,290 + rsxC b1629 0.16 -1.4
1,705,308 - rsxC b1629 0.16 +1.0
1,705,308 - rsxC b1629 0.16 +0.9
1,705,459 + rsxC b1629 0.23 -0.2
1,705,459 + rsxC b1629 0.23 +2.0
1,705,529 - rsxC b1629 0.26 -2.6
1,705,579 + rsxC b1629 0.29 +1.4
1,705,727 - rsxC b1629 0.35 -1.8
1,705,812 + rsxC b1629 0.39 +0.5
1,705,999 + rsxC b1629 0.48 +0.8
1,706,363 + rsxC b1629 0.64 +0.6
1,706,547 - rsxC b1629 0.72 +0.8
1,706,829 + rsxC b1629 0.85 -0.2
1,706,859 + rsxC b1629 0.86 -0.3
1,706,859 + rsxC b1629 0.86 +0.4
1,706,883 - rsxC b1629 0.87 +0.7
1,706,883 - rsxC b1629 0.87 +0.1
1,706,924 + rsxC b1629 0.89 -0.6
1,707,252 + -1.4
1,707,252 + -0.2
1,707,328 + rsxD b1630 0.15 +1.0
1,707,414 + rsxD b1630 0.23 +0.4
1,707,414 + rsxD b1630 0.23 +0.9
1,707,473 - rsxD b1630 0.29 +0.7
1,707,579 - rsxD b1630 0.39 +0.1
1,707,696 - rsxD b1630 0.50 +1.4
1,707,716 + rsxD b1630 0.52 +0.1
1,707,793 + rsxD b1630 0.59 +0.1
1,707,803 - rsxD b1630 0.60 -0.3
1,707,895 + rsxD b1630 0.69 -0.6
1,707,914 - rsxD b1630 0.71 +3.0
1,707,924 + rsxD b1630 0.72 +1.4
1,708,021 + rsxD b1630 0.81 +0.4
1,708,161 + +0.4
1,708,161 + +0.5

Or see this region's nucleotide sequence