Experiment: LB Aerobic with Spiramycin 0.000125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ybhM and ybhN are separated by 35 nucleotides ybhN and ybhO overlap by 1 nucleotides ybhO and ybhP overlap by 4 nucleotides ybhP and ybhQ are separated by 132 nucleotides
b0787: ybhM - conserved inner membrane protein (NCBI), at 820,016 to 820,729
ybhM
b0788: ybhN - conserved inner membrane protein (NCBI), at 820,765 to 821,721
ybhN
b0789: ybhO - cardiolipin synthase 2 (NCBI), at 821,721 to 822,962
ybhO
b0790: ybhP - predicted DNase (NCBI), at 822,959 to 823,720
ybhP
b0791: ybhQ - predicted inner membrane protein (NCBI), at 823,853 to 824,263
ybhQ
Position (kb) 821
822
823 Strain fitness (log2 ratio) -2
-1
0
1 at 820.826 kb on - strand at 820.826 kb on - strand at 820.826 kb on - strand at 820.926 kb on - strand, within ybhN at 821.003 kb on + strand, within ybhN at 821.004 kb on - strand, within ybhN at 821.158 kb on - strand, within ybhN at 821.314 kb on - strand, within ybhN at 821.329 kb on + strand, within ybhN at 821.333 kb on - strand, within ybhN at 821.435 kb on - strand, within ybhN at 821.615 kb on - strand, within ybhN at 821.666 kb on - strand at 821.850 kb on - strand, within ybhO at 821.898 kb on - strand, within ybhO at 821.907 kb on - strand, within ybhO at 822.100 kb on + strand, within ybhO at 822.100 kb on + strand, within ybhO at 822.155 kb on - strand, within ybhO at 822.188 kb on - strand, within ybhO at 822.188 kb on - strand, within ybhO at 822.273 kb on + strand, within ybhO at 822.328 kb on - strand, within ybhO at 822.328 kb on - strand, within ybhO at 822.553 kb on + strand, within ybhO at 822.553 kb on + strand, within ybhO at 822.780 kb on + strand, within ybhO at 822.780 kb on + strand, within ybhO at 822.849 kb on + strand at 822.891 kb on - strand at 823.224 kb on + strand, within ybhP at 823.224 kb on + strand, within ybhP at 823.296 kb on - strand, within ybhP at 823.296 kb on - strand, within ybhP at 823.302 kb on - strand, within ybhP at 823.331 kb on + strand, within ybhP at 823.346 kb on + strand, within ybhP at 823.405 kb on + strand, within ybhP at 823.434 kb on - strand, within ybhP at 823.454 kb on - strand, within ybhP at 823.604 kb on - strand, within ybhP at 823.653 kb on - strand at 823.850 kb on + strand at 823.893 kb on - strand at 823.947 kb on + strand, within ybhQ at 823.961 kb on - strand, within ybhQ
Per-strain Table
Position Strand Gene LocusTag Fraction LB Aerobic with Spiramycin 0.000125 mM remove 820,826 - -0.6 820,826 - +0.4 820,826 - -2.1 820,926 - ybhN b0788 0.17 -0.3 821,003 + ybhN b0788 0.25 +0.6 821,004 - ybhN b0788 0.25 +0.0 821,158 - ybhN b0788 0.41 -0.1 821,314 - ybhN b0788 0.57 -0.6 821,329 + ybhN b0788 0.59 +0.0 821,333 - ybhN b0788 0.59 +0.5 821,435 - ybhN b0788 0.70 +0.0 821,615 - ybhN b0788 0.89 +0.5 821,666 - +0.3 821,850 - ybhO b0789 0.10 +0.7 821,898 - ybhO b0789 0.14 +0.5 821,907 - ybhO b0789 0.15 -1.9 822,100 + ybhO b0789 0.31 -0.7 822,100 + ybhO b0789 0.31 +0.1 822,155 - ybhO b0789 0.35 -1.1 822,188 - ybhO b0789 0.38 +0.6 822,188 - ybhO b0789 0.38 +0.5 822,273 + ybhO b0789 0.44 +0.1 822,328 - ybhO b0789 0.49 +1.0 822,328 - ybhO b0789 0.49 -0.2 822,553 + ybhO b0789 0.67 +1.0 822,553 + ybhO b0789 0.67 -0.4 822,780 + ybhO b0789 0.85 -0.2 822,780 + ybhO b0789 0.85 +0.5 822,849 + -0.1 822,891 - -0.2 823,224 + ybhP b0790 0.35 -0.8 823,224 + ybhP b0790 0.35 +0.6 823,296 - ybhP b0790 0.44 -0.4 823,296 - ybhP b0790 0.44 -0.2 823,302 - ybhP b0790 0.45 -0.3 823,331 + ybhP b0790 0.49 -1.1 823,346 + ybhP b0790 0.51 -0.3 823,405 + ybhP b0790 0.59 +0.3 823,434 - ybhP b0790 0.62 +0.0 823,454 - ybhP b0790 0.65 +0.4 823,604 - ybhP b0790 0.85 -0.4 823,653 - +0.4 823,850 + -0.1 823,893 - -1.4 823,947 + ybhQ b0791 0.23 +0.1 823,961 - ybhQ b0791 0.26 -1.0
Or see this region's nucleotide sequence