Strain Fitness in Pseudomonas putida KT2440 around PP_4322

Experiment: D-Glucose (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_5748 and PP_4321 overlap by 4 nucleotidesPP_4321 and PP_4322 overlap by 4 nucleotidesPP_4322 and PP_4323 overlap by 4 nucleotidesPP_4323 and PP_4324 overlap by 4 nucleotidesPP_4324 and PP_4325 overlap by 4 nucleotides PP_4320: PP_4320 - cytochrome c-type biogenesis protein, at 4,910,515 to 4,911,711 _4320 PP_5748: PP_5748 - cytochrome c heme lyase subunit, at 4,911,704 to 4,912,180 _5748 PP_4321: PP_4321 - holocytochrome c synthetase subunit, at 4,912,177 to 4,912,713 _4321 PP_4322: PP_4322 - holocytochrome c synthetase, at 4,912,710 to 4,914,698 _4322 PP_4323: PP_4323 - Cytochrome c-type biogenesis protein CcmE, at 4,914,695 to 4,915,150 _4323 PP_4324: PP_4324 - Heme exporter protein D, at 4,915,147 to 4,915,323 _4324 PP_4325: PP_4325 - protoheme IX reservoir complex subunit, at 4,915,320 to 4,916,087 _4325 Position (kb) 4912 4913 4914 4915Strain fitness (log2 ratio) -1 0 1 2at 4912.108 kb on - strand, within PP_5748at 4912.108 kb on - strand, within PP_5748at 4912.314 kb on - strandat 4912.854 kb on - strandat 4912.858 kb on - strandat 4914.152 kb on - strand, within PP_4322at 4915.038 kb on + strand, within PP_4323at 4915.039 kb on - strand, within PP_4323at 4915.354 kb on - strandat 4915.354 kb on - strandat 4915.652 kb on + strand, within PP_4325

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Glucose (C)
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4,912,108 - PP_5748 0.85 +0.9
4,912,108 - PP_5748 0.85 +0.1
4,912,314 - -0.5
4,912,854 - -1.4
4,912,858 - +0.6
4,914,152 - PP_4322 0.72 +2.6
4,915,038 + PP_4323 0.75 -0.4
4,915,039 - PP_4323 0.75 +2.2
4,915,354 - +0.2
4,915,354 - +0.2
4,915,652 + PP_4325 0.43 +0.1

Or see this region's nucleotide sequence