Experiment: D-Glucose (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0597 and PP_16SD are separated by 750 nucleotides PP_16SD and PP_t05 are separated by 85 nucleotides PP_t05 and PP_t06 are separated by 24 nucleotides PP_t06 and PP_23SD are separated by 243 nucleotides
PP_0597: PP_0597 - methylmalonate-semialdehyde dehydrogenase, at 695,580 to 697,073
_0597
PP_16SD: PP_16SD - 16S ribosomal RNA, at 697,824 to 699,345
_16SD
PP_t05: PP_t05 - tRNA-Ile, at 699,431 to 699,507
_t05
PP_t06: PP_t06 - tRNA-Ala, at 699,532 to 699,607
_t06
PP_23SD: PP_23SD - 23S ribosomal RNA, at 699,851 to 702,752
_23SD
Position (kb) 697
698
699
700 Strain fitness (log2 ratio) -2
-1
0
1 at 696.824 kb on + strand, within PP_0597 at 696.825 kb on - strand, within PP_0597 at 696.825 kb on - strand, within PP_0597 at 696.875 kb on + strand, within PP_0597 at 696.875 kb on + strand, within PP_0597 at 696.876 kb on - strand, within PP_0597 at 696.876 kb on - strand, within PP_0597 at 696.893 kb on - strand, within PP_0597 at 696.893 kb on - strand, within PP_0597 at 696.966 kb on + strand at 696.967 kb on - strand at 696.990 kb on + strand at 696.990 kb on + strand at 696.991 kb on - strand at 696.991 kb on - strand at 697.072 kb on - strand at 697.072 kb on - strand at 697.080 kb on + strand at 697.081 kb on - strand at 697.353 kb on + strand at 697.353 kb on + strand at 698.231 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose (C) remove 696,824 + PP_0597 0.83 -0.1 696,825 - PP_0597 0.83 -0.4 696,825 - PP_0597 0.83 +0.6 696,875 + PP_0597 0.87 -0.7 696,875 + PP_0597 0.87 -0.1 696,876 - PP_0597 0.87 -1.1 696,876 - PP_0597 0.87 +0.3 696,893 - PP_0597 0.88 -1.3 696,893 - PP_0597 0.88 -1.7 696,966 + +0.4 696,967 - -0.2 696,990 + +0.3 696,990 + +0.8 696,991 - -1.0 696,991 - +0.1 697,072 - -0.1 697,072 - +0.5 697,080 + -0.6 697,081 - -2.3 697,353 + -0.4 697,353 + -0.5 698,231 + -1.1
Or see this region's nucleotide sequence