Experiment: D,L-Malic Acid (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu0098 and Atu0099 are separated by 15 nucleotides Atu0099 and Atu0100 are separated by 11 nucleotides Atu0100 and Atu0101 are separated by 150 nucleotides Atu0101 and Atu0102 are separated by 94 nucleotides
Atu0098: Atu0098 - mutT/nudix family protein, at 102,038 to 103,000
Atu0098
Atu0099: Atu0099 - prephenate dehydratase, at 103,016 to 103,879
Atu0099
Atu0100: Atu0100 - 3-deoxy-manno-octulosonate cytidylyltransferase, at 103,891 to 104,646
Atu0100
Atu0101: Atu0101 - cytochrome c, at 104,797 to 105,375
Atu0101
Atu0102: Atu0102 - exopolysaccharide production protein, at 105,470 to 106,195
Atu0102
Position (kb) 103
104
105 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 102.998 kb on - strand at 103.022 kb on - strand at 103.031 kb on - strand at 103.110 kb on + strand, within Atu0099 at 103.191 kb on - strand, within Atu0099 at 103.239 kb on + strand, within Atu0099 at 103.247 kb on - strand, within Atu0099 at 103.247 kb on - strand, within Atu0099 at 103.247 kb on - strand, within Atu0099 at 103.270 kb on - strand, within Atu0099 at 103.448 kb on - strand, within Atu0099 at 103.471 kb on + strand, within Atu0099 at 103.507 kb on + strand, within Atu0099 at 103.555 kb on + strand, within Atu0099 at 103.562 kb on + strand, within Atu0099 at 103.692 kb on + strand, within Atu0099 at 103.760 kb on - strand, within Atu0099 at 104.661 kb on - strand at 104.732 kb on - strand at 104.769 kb on - strand at 105.097 kb on - strand, within Atu0101 at 105.136 kb on + strand, within Atu0101 at 105.146 kb on + strand, within Atu0101 at 105.149 kb on + strand, within Atu0101 at 105.287 kb on + strand, within Atu0101 at 105.372 kb on - strand at 105.374 kb on - strand at 105.395 kb on - strand at 105.526 kb on - strand at 105.552 kb on + strand, within Atu0102 at 105.552 kb on + strand, within Atu0102
Per-strain Table
Position Strand Gene LocusTag Fraction D,L-Malic Acid (C) remove 102,998 - +0.2 103,022 - +0.9 103,031 - -0.4 103,110 + Atu0099 0.11 -6.0 103,191 - Atu0099 0.20 -3.5 103,239 + Atu0099 0.26 -4.0 103,247 - Atu0099 0.27 -4.9 103,247 - Atu0099 0.27 -4.2 103,247 - Atu0099 0.27 -0.4 103,270 - Atu0099 0.29 -3.8 103,448 - Atu0099 0.50 -0.9 103,471 + Atu0099 0.53 -5.5 103,507 + Atu0099 0.57 -2.4 103,555 + Atu0099 0.62 -2.8 103,562 + Atu0099 0.63 -3.4 103,692 + Atu0099 0.78 -5.0 103,760 - Atu0099 0.86 -5.6 104,661 - -0.1 104,732 - +0.4 104,769 - +0.2 105,097 - Atu0101 0.52 -0.3 105,136 + Atu0101 0.59 -0.3 105,146 + Atu0101 0.60 -1.6 105,149 + Atu0101 0.61 -0.3 105,287 + Atu0101 0.85 +0.4 105,372 - -0.4 105,374 - -0.6 105,395 - +0.3 105,526 - -0.3 105,552 + Atu0102 0.11 -0.3 105,552 + Atu0102 0.11 -0.8
Or see this region's nucleotide sequence