Experiment: D,L-Malic Acid (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu0015 and Atu0016 are separated by 69 nucleotides Atu0016 and Atu0017 are separated by 102 nucleotides Atu0017 and Atu0018 are separated by 1 nucleotides
Atu0015: Atu0015 - intracellular PHB depolymerase, at 12,339 to 13,535
Atu0015
Atu0016: Atu0016 - hypothetical protein, at 13,605 to 14,372
Atu0016
Atu0017: Atu0017 - N-(5-phosphoribosyl) anthranilate isomerase, at 14,475 to 15,137
Atu0017
Atu0018: Atu0018 - tryptophan synthase beta subunit, at 15,139 to 16,359
Atu0018
Position (kb) 13
14
15 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 12.665 kb on - strand, within Atu0015 at 12.679 kb on - strand, within Atu0015 at 12.681 kb on + strand, within Atu0015 at 12.946 kb on - strand, within Atu0015 at 13.045 kb on + strand, within Atu0015 at 13.074 kb on + strand, within Atu0015 at 13.205 kb on - strand, within Atu0015 at 13.296 kb on + strand, within Atu0015 at 13.336 kb on - strand, within Atu0015 at 13.381 kb on - strand, within Atu0015 at 13.433 kb on - strand at 13.468 kb on - strand at 13.483 kb on + strand at 13.609 kb on - strand at 14.690 kb on - strand, within Atu0017 at 14.703 kb on + strand, within Atu0017 at 14.819 kb on + strand, within Atu0017 at 14.946 kb on - strand, within Atu0017 at 14.987 kb on + strand, within Atu0017 at 15.033 kb on - strand, within Atu0017
Per-strain Table
Position Strand Gene LocusTag Fraction D,L-Malic Acid (C) remove 12,665 - Atu0015 0.27 +0.2 12,679 - Atu0015 0.28 -0.5 12,681 + Atu0015 0.29 +1.6 12,946 - Atu0015 0.51 -0.3 13,045 + Atu0015 0.59 +0.1 13,074 + Atu0015 0.61 +0.2 13,205 - Atu0015 0.72 +0.3 13,296 + Atu0015 0.80 -0.2 13,336 - Atu0015 0.83 -0.9 13,381 - Atu0015 0.87 +0.1 13,433 - +0.2 13,468 - -0.4 13,483 + -0.3 13,609 - -0.3 14,690 - Atu0017 0.32 -0.5 14,703 + Atu0017 0.34 -3.4 14,819 + Atu0017 0.52 -1.7 14,946 - Atu0017 0.71 -3.4 14,987 + Atu0017 0.77 -4.4 15,033 - Atu0017 0.84 -1.2
Or see this region's nucleotide sequence