Strain Fitness in Escherichia coli BW25113 around b3009

Experiment: LB Aerobic with Phenazine ethosulfate 0.0000625 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntmetC and yghB are separated by 139 nucleotidesyghB and yqhC are separated by 39 nucleotides b3008: metC - cystathionine beta-lyase (NCBI), at 3,150,258 to 3,151,445 metC b3009: yghB - conserved inner membrane protein (NCBI), at 3,151,585 to 3,152,244 yghB b3010: yqhC - putative ARAC-type regulatory protein (VIMSS), at 3,152,284 to 3,153,240 yqhC Position (kb) 3151 3152 3153Strain fitness (log2 ratio) -2 -1 0 1 2 3at 3150.640 kb on - strand, within metCat 3150.654 kb on + strand, within metCat 3150.669 kb on + strand, within metCat 3150.706 kb on - strand, within metCat 3150.791 kb on - strand, within metCat 3150.820 kb on + strand, within metCat 3150.820 kb on + strand, within metCat 3150.912 kb on + strand, within metCat 3150.914 kb on + strand, within metCat 3150.915 kb on + strand, within metCat 3150.932 kb on - strand, within metCat 3151.015 kb on + strandat 3151.015 kb on + strand, within metCat 3151.028 kb on + strand, within metCat 3151.038 kb on - strand, within metCat 3151.038 kb on - strand, within metCat 3151.074 kb on - strand, within metCat 3151.074 kb on - strand, within metCat 3151.120 kb on + strand, within metCat 3151.125 kb on + strand, within metCat 3151.138 kb on - strand, within metCat 3151.216 kb on + strand, within metCat 3151.216 kb on + strand, within metCat 3151.237 kb on + strand, within metCat 3151.274 kb on - strand, within metCat 3151.376 kb on + strandat 3151.655 kb on - strand, within yghBat 3151.859 kb on + strand, within yghBat 3151.859 kb on + strand, within yghBat 3151.867 kb on - strand, within yghBat 3151.889 kb on + strand, within yghBat 3151.889 kb on + strand, within yghBat 3152.046 kb on + strand, within yghBat 3152.046 kb on + strand, within yghBat 3152.076 kb on + strand, within yghBat 3152.084 kb on - strand, within yghBat 3152.101 kb on - strand, within yghBat 3152.101 kb on - strand, within yghBat 3152.130 kb on + strand, within yghBat 3152.133 kb on + strand, within yghBat 3152.152 kb on - strand, within yghBat 3152.153 kb on - strand, within yghBat 3152.186 kb on + strandat 3152.256 kb on + strandat 3152.256 kb on + strandat 3152.280 kb on + strandat 3152.290 kb on + strandat 3152.290 kb on + strandat 3152.372 kb on + strandat 3152.377 kb on + strandat 3152.608 kb on - strand, within yqhCat 3152.608 kb on - strand, within yqhCat 3152.615 kb on + strand, within yqhCat 3152.615 kb on + strand, within yqhCat 3152.628 kb on - strand, within yqhCat 3152.630 kb on - strand, within yqhCat 3152.635 kb on - strand, within yqhCat 3152.728 kb on - strand, within yqhCat 3152.738 kb on - strand, within yqhCat 3152.837 kb on - strand, within yqhCat 3152.922 kb on - strand, within yqhCat 3153.056 kb on - strand, within yqhC

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Aerobic with Phenazine ethosulfate 0.0000625 mM
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3,150,640 - metC b3008 0.32 +0.3
3,150,654 + metC b3008 0.33 -0.3
3,150,669 + metC b3008 0.35 -1.6
3,150,706 - metC b3008 0.38 +0.0
3,150,791 - metC b3008 0.45 +0.5
3,150,820 + metC b3008 0.47 -0.1
3,150,820 + metC b3008 0.47 -0.9
3,150,912 + metC b3008 0.55 -1.8
3,150,914 + metC b3008 0.55 -1.4
3,150,915 + metC b3008 0.55 +1.5
3,150,932 - metC b3008 0.57 +0.3
3,151,015 + +2.6
3,151,015 + metC b3008 0.64 +0.5
3,151,028 + metC b3008 0.65 -0.8
3,151,038 - metC b3008 0.66 +0.7
3,151,038 - metC b3008 0.66 -0.1
3,151,074 - metC b3008 0.69 +0.4
3,151,074 - metC b3008 0.69 -0.3
3,151,120 + metC b3008 0.73 -0.6
3,151,125 + metC b3008 0.73 +1.9
3,151,138 - metC b3008 0.74 +0.3
3,151,216 + metC b3008 0.81 +1.6
3,151,216 + metC b3008 0.81 +0.0
3,151,237 + metC b3008 0.82 -0.0
3,151,274 - metC b3008 0.86 -0.4
3,151,376 + -0.3
3,151,655 - yghB b3009 0.11 +0.0
3,151,859 + yghB b3009 0.42 +1.5
3,151,859 + yghB b3009 0.42 +1.1
3,151,867 - yghB b3009 0.43 +2.6
3,151,889 + yghB b3009 0.46 +0.3
3,151,889 + yghB b3009 0.46 +2.0
3,152,046 + yghB b3009 0.70 +1.0
3,152,046 + yghB b3009 0.70 +1.9
3,152,076 + yghB b3009 0.74 +0.7
3,152,084 - yghB b3009 0.76 +1.1
3,152,101 - yghB b3009 0.78 +1.7
3,152,101 - yghB b3009 0.78 +2.8
3,152,130 + yghB b3009 0.83 +1.3
3,152,133 + yghB b3009 0.83 +2.0
3,152,152 - yghB b3009 0.86 +1.7
3,152,153 - yghB b3009 0.86 +0.7
3,152,186 + +0.3
3,152,256 + +0.3
3,152,256 + -1.6
3,152,280 + -0.3
3,152,290 + -0.2
3,152,290 + +0.3
3,152,372 + -0.2
3,152,377 + +0.0
3,152,608 - yqhC b3010 0.34 -0.1
3,152,608 - yqhC b3010 0.34 +0.2
3,152,615 + yqhC b3010 0.35 +0.1
3,152,615 + yqhC b3010 0.35 +0.5
3,152,628 - yqhC b3010 0.36 -0.3
3,152,630 - yqhC b3010 0.36 +0.1
3,152,635 - yqhC b3010 0.37 -0.4
3,152,728 - yqhC b3010 0.46 -0.8
3,152,738 - yqhC b3010 0.47 -0.3
3,152,837 - yqhC b3010 0.58 -0.7
3,152,922 - yqhC b3010 0.67 +0.0
3,153,056 - yqhC b3010 0.81 +0.8

Or see this region's nucleotide sequence