Experiment: LB Aerobic with Phenazine ethosulfate 0.0000625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt speE and yacC are separated by 105 nucleotides yacC and cueO are separated by 165 nucleotides cueO and gcd are separated by 201 nucleotides
b0121: speE - spermidine synthase (NCBI), at 135,598 to 136,464
speE
b0122: yacC - orf, hypothetical protein (VIMSS), at 136,570 to 136,917
yacC
b0123: cueO - multicopper oxidase (laccase) (NCBI), at 137,083 to 138,633
cueO
b0124: gcd - glucose dehydrogenase (NCBI), at 138,835 to 141,225
gcd
Position (kb)
137
138
139 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 136.088 kb on + strand, within speE at 136.096 kb on - strand, within speE at 136.148 kb on + strand, within speE at 136.189 kb on + strand, within speE at 136.191 kb on + strand, within speE at 136.257 kb on - strand, within speE at 136.402 kb on - strand at 136.402 kb on - strand at 136.424 kb on - strand at 136.516 kb on - strand at 136.516 kb on - strand at 136.733 kb on + strand, within yacC at 136.755 kb on - strand, within yacC at 136.779 kb on + strand, within yacC at 136.779 kb on + strand, within yacC at 136.859 kb on + strand, within yacC at 136.901 kb on - strand at 136.922 kb on + strand at 136.922 kb on + strand at 137.033 kb on + strand at 137.123 kb on + strand at 137.199 kb on + strand at 137.240 kb on + strand, within cueO at 137.240 kb on + strand, within cueO at 137.380 kb on - strand, within cueO at 137.401 kb on + strand, within cueO at 137.401 kb on - strand, within cueO at 137.401 kb on - strand, within cueO at 137.482 kb on - strand, within cueO at 137.485 kb on + strand, within cueO at 137.544 kb on - strand, within cueO at 137.544 kb on - strand, within cueO at 137.708 kb on + strand, within cueO at 137.708 kb on + strand, within cueO at 137.730 kb on - strand, within cueO at 137.730 kb on - strand, within cueO at 137.735 kb on - strand, within cueO at 137.852 kb on - strand, within cueO at 137.889 kb on + strand, within cueO at 137.897 kb on + strand, within cueO at 137.907 kb on + strand, within cueO at 137.913 kb on - strand, within cueO at 138.067 kb on + strand, within cueO at 138.067 kb on + strand, within cueO at 138.070 kb on + strand, within cueO at 138.070 kb on + strand, within cueO at 138.232 kb on + strand, within cueO at 138.242 kb on + strand, within cueO at 138.252 kb on + strand, within cueO at 138.255 kb on - strand, within cueO at 138.352 kb on - strand, within cueO at 138.418 kb on + strand, within cueO at 138.418 kb on + strand, within cueO at 138.435 kb on - strand, within cueO at 138.435 kb on - strand, within cueO at 138.566 kb on + strand at 138.566 kb on + strand at 138.572 kb on - strand at 138.589 kb on - strand at 138.689 kb on - strand at 138.797 kb on - strand at 138.862 kb on + strand at 138.862 kb on + strand at 138.870 kb on - strand at 138.870 kb on - strand at 138.870 kb on - strand at 138.870 kb on - strand at 138.919 kb on - strand at 138.928 kb on + strand at 138.928 kb on + strand at 138.950 kb on - strand at 139.025 kb on + strand at 139.025 kb on + strand at 139.057 kb on - strand at 139.095 kb on - strand, within gcd at 139.095 kb on - strand, within gcd at 139.147 kb on + strand, within gcd at 139.203 kb on + strand, within gcd at 139.211 kb on + strand, within gcd at 139.211 kb on + strand, within gcd at 139.243 kb on + strand, within gcd at 139.245 kb on - strand, within gcd at 139.245 kb on - strand, within gcd at 139.267 kb on + strand, within gcd at 139.274 kb on + strand, within gcd at 139.282 kb on - strand, within gcd at 139.282 kb on - strand, within gcd at 139.284 kb on + strand, within gcd at 139.284 kb on + strand, within gcd at 139.285 kb on - strand, within gcd at 139.285 kb on - strand, within gcd at 139.288 kb on + strand, within gcd at 139.352 kb on - strand, within gcd at 139.405 kb on - strand, within gcd at 139.405 kb on - strand, within gcd at 139.469 kb on - strand, within gcd at 139.469 kb on - strand, within gcd at 139.492 kb on - strand, within gcd at 139.492 kb on - strand, within gcd at 139.552 kb on + strand, within gcd at 139.623 kb on + strand, within gcd at 139.623 kb on + strand, within gcd
Per-strain Table
Position Strand Gene LocusTag Fraction LB Aerobic with Phenazine ethosulfate 0.0000625 mM remove 136,088 + speE b0121 0.57 +0.1 136,096 - speE b0121 0.57 +1.8 136,148 + speE b0121 0.63 -0.9 136,189 + speE b0121 0.68 +0.2 136,191 + speE b0121 0.68 -0.6 136,257 - speE b0121 0.76 -0.6 136,402 - +0.7 136,402 - +1.7 136,424 - +0.0 136,516 - -1.7 136,516 - +0.2 136,733 + yacC b0122 0.47 -1.7 136,755 - yacC b0122 0.53 -1.0 136,779 + yacC b0122 0.60 -1.1 136,779 + yacC b0122 0.60 -1.8 136,859 + yacC b0122 0.83 -1.8 136,901 - -1.0 136,922 + -1.6 136,922 + -1.1 137,033 + -0.5 137,123 + -0.7 137,199 + -0.3 137,240 + cueO b0123 0.10 -1.4 137,240 + cueO b0123 0.10 -1.8 137,380 - cueO b0123 0.19 +1.0 137,401 + cueO b0123 0.21 -0.3 137,401 - cueO b0123 0.21 +0.1 137,401 - cueO b0123 0.21 +1.1 137,482 - cueO b0123 0.26 -0.6 137,485 + cueO b0123 0.26 -0.2 137,544 - cueO b0123 0.30 -1.1 137,544 - cueO b0123 0.30 -0.5 137,708 + cueO b0123 0.40 +0.1 137,708 + cueO b0123 0.40 +0.6 137,730 - cueO b0123 0.42 +0.5 137,730 - cueO b0123 0.42 -0.1 137,735 - cueO b0123 0.42 +0.6 137,852 - cueO b0123 0.50 +0.2 137,889 + cueO b0123 0.52 +0.1 137,897 + cueO b0123 0.52 +0.7 137,907 + cueO b0123 0.53 +0.5 137,913 - cueO b0123 0.54 -1.2 138,067 + cueO b0123 0.63 +0.7 138,067 + cueO b0123 0.63 +0.8 138,070 + cueO b0123 0.64 +0.3 138,070 + cueO b0123 0.64 -0.4 138,232 + cueO b0123 0.74 -1.0 138,242 + cueO b0123 0.75 -1.1 138,252 + cueO b0123 0.75 -0.0 138,255 - cueO b0123 0.76 -0.2 138,352 - cueO b0123 0.82 -0.6 138,418 + cueO b0123 0.86 -0.5 138,418 + cueO b0123 0.86 +0.5 138,435 - cueO b0123 0.87 -0.8 138,435 - cueO b0123 0.87 -1.7 138,566 + -0.8 138,566 + -1.7 138,572 - +0.8 138,589 - +0.2 138,689 - -0.5 138,797 - +0.3 138,862 + +0.5 138,862 + -0.5 138,870 - +0.5 138,870 - +0.4 138,870 - -0.5 138,870 - +0.1 138,919 - -0.5 138,928 + +1.2 138,928 + +0.3 138,950 - -0.1 139,025 + -0.4 139,025 + -0.2 139,057 - +1.2 139,095 - gcd b0124 0.11 -1.2 139,095 - gcd b0124 0.11 +0.4 139,147 + gcd b0124 0.13 -1.5 139,203 + gcd b0124 0.15 -1.0 139,211 + gcd b0124 0.16 -2.3 139,211 + gcd b0124 0.16 +0.2 139,243 + gcd b0124 0.17 -0.2 139,245 - gcd b0124 0.17 +0.5 139,245 - gcd b0124 0.17 -0.6 139,267 + gcd b0124 0.18 -0.0 139,274 + gcd b0124 0.18 -0.2 139,282 - gcd b0124 0.19 +0.5 139,282 - gcd b0124 0.19 -0.7 139,284 + gcd b0124 0.19 -1.0 139,284 + gcd b0124 0.19 -0.1 139,285 - gcd b0124 0.19 -1.6 139,285 - gcd b0124 0.19 +0.5 139,288 + gcd b0124 0.19 +0.2 139,352 - gcd b0124 0.22 -0.1 139,405 - gcd b0124 0.24 +0.5 139,405 - gcd b0124 0.24 -1.1 139,469 - gcd b0124 0.27 -0.5 139,469 - gcd b0124 0.27 +2.0 139,492 - gcd b0124 0.27 -0.5 139,492 - gcd b0124 0.27 -2.2 139,552 + gcd b0124 0.30 -0.9 139,623 + gcd b0124 0.33 -1.0 139,623 + gcd b0124 0.33 +0.8
Or see this region's nucleotide sequence