Strain Fitness in Escherichia coli BW25113 around b0065

Experiment: LB Aerobic with Phenazine ethosulfate 0.0000625 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntaraC and yabI are separated by 85 nucleotidesyabI and thiQ are separated by 113 nucleotidesthiQ and thiP overlap by 17 nucleotides b0064: araC - DNA-binding transcriptional dual regulator (NCBI), at 70,387 to 71,265 araC b0065: yabI - conserved inner membrane protein (NCBI), at 71,351 to 72,115 yabI b0066: thiQ - thiamin transporter subunit (NCBI), at 72,229 to 72,927 thiQ b0067: thiP - thiamin ABC transporter membrane protein (NCBI), at 72,911 to 74,521 thiP Position (kb) 71 72 73Strain fitness (log2 ratio) -2 -1 0 1 2 3at 70.634 kb on + strand, within araCat 70.634 kb on + strand, within araCat 70.637 kb on - strand, within araCat 70.713 kb on - strand, within araCat 70.755 kb on - strand, within araCat 70.809 kb on + strand, within araCat 70.809 kb on + strand, within araCat 70.815 kb on - strand, within araCat 70.829 kb on - strand, within araCat 70.834 kb on - strand, within araCat 70.834 kb on - strand, within araCat 70.874 kb on + strand, within araCat 70.885 kb on + strand, within araCat 70.949 kb on - strand, within araCat 71.118 kb on - strand, within araCat 71.118 kb on - strand, within araCat 71.119 kb on + strand, within araCat 71.168 kb on - strand, within araCat 71.240 kb on + strandat 71.303 kb on + strandat 71.303 kb on + strandat 71.307 kb on - strandat 71.307 kb on - strandat 71.345 kb on + strandat 71.421 kb on - strandat 71.431 kb on - strand, within yabIat 71.431 kb on - strand, within yabIat 71.448 kb on + strand, within yabIat 71.448 kb on + strand, within yabIat 71.466 kb on - strand, within yabIat 71.466 kb on - strand, within yabIat 71.592 kb on + strand, within yabIat 71.654 kb on + strand, within yabIat 71.723 kb on - strand, within yabIat 71.723 kb on - strand, within yabIat 71.745 kb on - strand, within yabIat 71.910 kb on + strand, within yabIat 71.976 kb on - strand, within yabIat 72.058 kb on - strandat 72.058 kb on - strandat 72.077 kb on + strandat 72.081 kb on + strandat 72.081 kb on + strandat 72.116 kb on - strandat 72.117 kb on + strandat 72.117 kb on + strandat 72.118 kb on - strandat 72.159 kb on - strandat 72.181 kb on + strandat 72.203 kb on - strandat 72.212 kb on + strandat 72.212 kb on + strandat 72.239 kb on + strandat 72.239 kb on + strandat 72.247 kb on - strandat 72.249 kb on + strandat 72.249 kb on + strandat 72.249 kb on + strandat 72.257 kb on + strandat 72.272 kb on - strandat 72.321 kb on + strand, within thiQat 72.321 kb on + strand, within thiQat 72.354 kb on + strand, within thiQat 72.364 kb on + strand, within thiQat 72.370 kb on - strand, within thiQat 72.372 kb on - strand, within thiQat 72.372 kb on - strand, within thiQat 72.372 kb on - strand, within thiQat 72.372 kb on - strand, within thiQat 72.379 kb on - strand, within thiQat 72.415 kb on - strand, within thiQat 72.500 kb on - strand, within thiQat 72.500 kb on - strand, within thiQat 72.513 kb on - strand, within thiQat 72.513 kb on - strand, within thiQat 72.587 kb on + strand, within thiQat 72.593 kb on - strand, within thiQat 72.607 kb on - strand, within thiQat 72.630 kb on + strand, within thiQat 72.734 kb on + strand, within thiQat 72.734 kb on + strand, within thiQat 72.742 kb on - strand, within thiQat 72.856 kb on + strand, within thiQat 72.878 kb on - strandat 72.878 kb on - strandat 73.002 kb on - strandat 73.002 kb on - strandat 73.102 kb on - strand, within thiP

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Aerobic with Phenazine ethosulfate 0.0000625 mM
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70,634 + araC b0064 0.28 -0.7
70,634 + araC b0064 0.28 +0.4
70,637 - araC b0064 0.28 +0.5
70,713 - araC b0064 0.37 -0.8
70,755 - araC b0064 0.42 -0.1
70,809 + araC b0064 0.48 -0.2
70,809 + araC b0064 0.48 -0.7
70,815 - araC b0064 0.49 -0.8
70,829 - araC b0064 0.50 -0.5
70,834 - araC b0064 0.51 +0.1
70,834 - araC b0064 0.51 -0.8
70,874 + araC b0064 0.55 -0.1
70,885 + araC b0064 0.57 -0.0
70,949 - araC b0064 0.64 -0.2
71,118 - araC b0064 0.83 +0.5
71,118 - araC b0064 0.83 +0.2
71,119 + araC b0064 0.83 -0.7
71,168 - araC b0064 0.89 +0.0
71,240 + +0.1
71,303 + -2.5
71,303 + +0.1
71,307 - +0.4
71,307 - -0.3
71,345 + -0.4
71,421 - +0.9
71,431 - yabI b0065 0.10 +3.1
71,431 - yabI b0065 0.10 -1.1
71,448 + yabI b0065 0.13 +1.5
71,448 + yabI b0065 0.13 +1.5
71,466 - yabI b0065 0.15 -0.6
71,466 - yabI b0065 0.15 +1.1
71,592 + yabI b0065 0.32 +0.1
71,654 + yabI b0065 0.40 +1.0
71,723 - yabI b0065 0.49 +1.5
71,723 - yabI b0065 0.49 +0.8
71,745 - yabI b0065 0.52 +0.5
71,910 + yabI b0065 0.73 +1.8
71,976 - yabI b0065 0.82 +0.4
72,058 - +3.0
72,058 - +0.7
72,077 + +2.1
72,081 + +1.5
72,081 + +0.1
72,116 - +0.4
72,117 + +0.2
72,117 + +0.9
72,118 - +0.3
72,159 - +0.2
72,181 + -0.4
72,203 - -0.7
72,212 + -1.2
72,212 + +0.2
72,239 + -0.4
72,239 + +0.4
72,247 - +0.0
72,249 + -0.6
72,249 + +0.8
72,249 + -0.0
72,257 + -0.8
72,272 - -0.4
72,321 + thiQ b0066 0.13 -0.5
72,321 + thiQ b0066 0.13 -0.1
72,354 + thiQ b0066 0.18 -0.9
72,364 + thiQ b0066 0.19 -2.3
72,370 - thiQ b0066 0.20 -1.3
72,372 - thiQ b0066 0.20 +0.9
72,372 - thiQ b0066 0.20 -1.8
72,372 - thiQ b0066 0.20 -0.2
72,372 - thiQ b0066 0.20 +0.8
72,379 - thiQ b0066 0.21 +0.4
72,415 - thiQ b0066 0.27 -0.3
72,500 - thiQ b0066 0.39 -0.1
72,500 - thiQ b0066 0.39 -0.5
72,513 - thiQ b0066 0.41 +1.4
72,513 - thiQ b0066 0.41 +0.5
72,587 + thiQ b0066 0.51 -1.2
72,593 - thiQ b0066 0.52 +0.4
72,607 - thiQ b0066 0.54 -0.8
72,630 + thiQ b0066 0.57 -0.3
72,734 + thiQ b0066 0.72 +0.2
72,734 + thiQ b0066 0.72 -1.9
72,742 - thiQ b0066 0.73 -0.5
72,856 + thiQ b0066 0.90 +0.5
72,878 - -0.4
72,878 - +0.6
73,002 - +0.1
73,002 - +0.6
73,102 - thiP b0067 0.12 -0.7

Or see this region's nucleotide sequence