Strain Fitness in Escherichia coli BW25113 around b3303

Experiment: LB, Aerobic

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntsecY and rplO are separated by 7 nucleotidesrplO and rpmD are separated by 3 nucleotidesrpmD and rpsE are separated by 3 nucleotidesrpsE and rplR are separated by 14 nucleotidesrplR and rplF are separated by 9 nucleotidesrplF and rpsH are separated by 12 nucleotides b3300: secY - protein translocase subunit SecY (NCBI), at 3,440,788 to 3,442,119 secY b3301: rplO - 50S ribosomal protein L15 (NCBI), at 3,442,127 to 3,442,561 rplO b3302: rpmD - 50S ribosomal protein L30 (NCBI), at 3,442,565 to 3,442,744 rpmD b3303: rpsE - 30S ribosomal protein S5 (NCBI), at 3,442,748 to 3,443,251 rpsE b3304: rplR - 50S ribosomal protein L18 (NCBI), at 3,443,266 to 3,443,619 rplR b3305: rplF - 50S ribosomal protein L6 (NCBI), at 3,443,629 to 3,444,162 rplF b3306: rpsH - 30S ribosomal protein S8 (NCBI), at 3,444,175 to 3,444,567 rpsH Position (kb) 3442 3443 3444Strain fitness (log2 ratio) -1 0 1 2at 3442.123 kb on - strandat 3442.123 kb on - strandat 3443.272 kb on - strandat 3443.272 kb on - strandat 3443.272 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB, Aerobic
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3,442,123 - +0.4
3,442,123 - +0.2
3,443,272 - +0.5
3,443,272 - -1.0
3,443,272 - +2.1

Or see this region's nucleotide sequence