Experiment: LB, Aerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt sspA and rpsI are separated by 394 nucleotides rpsI and rplM are separated by 15 nucleotides rplM and yhcM are separated by 218 nucleotides
b3229: sspA - stringent starvation protein A (NCBI), at 3,374,804 to 3,375,442
sspA
b3230: rpsI - 30S ribosomal protein S9 (NCBI), at 3,375,837 to 3,376,229
rpsI
b3231: rplM - 50S ribosomal protein L13 (NCBI), at 3,376,245 to 3,376,673
rplM
b3232: yhcM - conserved protein with nucleoside triphosphate hydrolase domain (NCBI), at 3,376,892 to 3,378,019
yhcM
Position (kb)
3375
3376
3377 Strain fitness (log2 ratio)
-1
0
1
2 at 3374.966 kb on - strand, within sspA at 3374.976 kb on + strand, within sspA at 3374.976 kb on + strand, within sspA at 3375.071 kb on - strand, within sspA at 3375.073 kb on - strand, within sspA at 3375.084 kb on - strand, within sspA at 3375.098 kb on + strand, within sspA at 3375.098 kb on + strand, within sspA at 3375.113 kb on - strand, within sspA at 3375.189 kb on - strand, within sspA at 3375.220 kb on - strand, within sspA at 3375.220 kb on - strand, within sspA at 3375.220 kb on - strand, within sspA at 3375.236 kb on - strand, within sspA at 3375.353 kb on + strand, within sspA at 3375.353 kb on + strand, within sspA at 3375.515 kb on - strand at 3376.719 kb on - strand at 3376.719 kb on - strand at 3376.844 kb on - strand at 3376.845 kb on - strand at 3376.929 kb on - strand at 3377.108 kb on + strand, within yhcM at 3377.108 kb on + strand, within yhcM at 3377.130 kb on - strand, within yhcM at 3377.162 kb on - strand, within yhcM at 3377.182 kb on - strand, within yhcM at 3377.191 kb on + strand, within yhcM at 3377.191 kb on + strand, within yhcM at 3377.193 kb on + strand, within yhcM at 3377.193 kb on + strand, within yhcM
Per-strain Table
Position Strand Gene LocusTag Fraction LB, Aerobic remove 3,374,966 - sspA b3229 0.25 -0.3 3,374,976 + sspA b3229 0.27 -0.5 3,374,976 + sspA b3229 0.27 +0.1 3,375,071 - sspA b3229 0.42 -1.6 3,375,073 - sspA b3229 0.42 +0.5 3,375,084 - sspA b3229 0.44 -1.4 3,375,098 + sspA b3229 0.46 +1.1 3,375,098 + sspA b3229 0.46 +0.5 3,375,113 - sspA b3229 0.48 -0.5 3,375,189 - sspA b3229 0.60 -0.3 3,375,220 - sspA b3229 0.65 +1.1 3,375,220 - sspA b3229 0.65 +0.5 3,375,220 - sspA b3229 0.65 -0.5 3,375,236 - sspA b3229 0.68 -0.3 3,375,353 + sspA b3229 0.86 +2.1 3,375,353 + sspA b3229 0.86 +0.5 3,375,515 - -0.6 3,376,719 - -0.3 3,376,719 - -0.4 3,376,844 - +0.5 3,376,845 - +0.2 3,376,929 - -1.2 3,377,108 + yhcM b3232 0.19 -0.6 3,377,108 + yhcM b3232 0.19 +0.5 3,377,130 - yhcM b3232 0.21 +0.1 3,377,162 - yhcM b3232 0.24 -1.2 3,377,182 - yhcM b3232 0.26 +0.4 3,377,191 + yhcM b3232 0.27 +0.1 3,377,191 + yhcM b3232 0.27 -0.2 3,377,193 + yhcM b3232 0.27 -0.1 3,377,193 + yhcM b3232 0.27 -0.1
Or see this region's nucleotide sequence