Strain Fitness in Escherichia coli BW25113 around b3168

Experiment: LB, Aerobic

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nttruB and rbfA overlap by 1 nucleotidesrbfA and infB are separated by 163 nucleotidesinfB and nusA are separated by 24 nucleotides b3166: truB - tRNA pseudouridine synthase B (NCBI), at 3,309,855 to 3,310,799 truB b3167: rbfA - ribosome-binding factor A (NCBI), at 3,310,799 to 3,311,200 rbfA b3168: infB - translation initiation factor IF-2 (NCBI), at 3,311,364 to 3,314,036 infB b3169: nusA - transcription elongation factor NusA (NCBI), at 3,314,061 to 3,315,548 nusA Position (kb) 3311 3312 3313 3314 3315Strain fitness (log2 ratio) -2 -1 0 1at 3310.371 kb on - strand, within truBat 3310.487 kb on + strand, within truBat 3310.523 kb on - strand, within truBat 3310.539 kb on + strand, within truBat 3310.545 kb on - strand, within truBat 3310.562 kb on + strand, within truBat 3310.562 kb on + strand, within truBat 3310.587 kb on + strand, within truBat 3310.655 kb on + strand, within truBat 3310.655 kb on + strand, within truBat 3310.682 kb on + strand, within truBat 3310.695 kb on - strand, within truBat 3310.700 kb on - strand, within truBat 3310.700 kb on - strand, within truBat 3310.723 kb on + strandat 3310.731 kb on - strandat 3310.736 kb on - strandat 3310.800 kb on - strandat 3310.800 kb on - strandat 3310.805 kb on - strandat 3310.845 kb on + strand, within rbfAat 3310.909 kb on - strand, within rbfAat 3311.290 kb on - strandat 3311.290 kb on - strandat 3311.382 kb on - strandat 3311.409 kb on - strandat 3311.409 kb on - strandat 3313.766 kb on - strand, within infBat 3313.842 kb on - strandat 3313.932 kb on - strandat 3313.976 kb on - strandat 3314.038 kb on - strandat 3314.051 kb on - strandat 3314.051 kb on - strandat 3314.233 kb on - strand, within nusAat 3314.249 kb on - strand, within nusAat 3314.252 kb on - strand, within nusAat 3314.302 kb on - strand, within nusAat 3314.302 kb on - strand, within nusAat 3314.302 kb on - strand, within nusA

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Per-strain Table

Position Strand Gene LocusTag Fraction LB, Aerobic
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3,310,371 - truB b3166 0.55 +1.1
3,310,487 + truB b3166 0.67 +0.2
3,310,523 - truB b3166 0.71 -0.3
3,310,539 + truB b3166 0.72 -0.9
3,310,545 - truB b3166 0.73 +0.6
3,310,562 + truB b3166 0.75 +0.3
3,310,562 + truB b3166 0.75 +0.0
3,310,587 + truB b3166 0.77 +0.1
3,310,655 + truB b3166 0.85 +0.6
3,310,655 + truB b3166 0.85 -2.0
3,310,682 + truB b3166 0.88 -0.7
3,310,695 - truB b3166 0.89 +0.4
3,310,700 - truB b3166 0.89 -0.3
3,310,700 - truB b3166 0.89 -0.4
3,310,723 + -0.8
3,310,731 - +0.5
3,310,736 - -1.0
3,310,800 - -0.7
3,310,800 - +0.4
3,310,805 - +0.7
3,310,845 + rbfA b3167 0.11 +0.0
3,310,909 - rbfA b3167 0.27 +0.2
3,311,290 - +0.4
3,311,290 - -0.6
3,311,382 - -0.4
3,311,409 - +1.0
3,311,409 - +0.4
3,313,766 - infB b3168 0.90 +1.0
3,313,842 - +1.0
3,313,932 - -1.0
3,313,976 - +0.4
3,314,038 - -0.3
3,314,051 - +0.1
3,314,051 - +0.3
3,314,233 - nusA b3169 0.12 -0.7
3,314,249 - nusA b3169 0.13 -2.1
3,314,252 - nusA b3169 0.13 +1.0
3,314,302 - nusA b3169 0.16 -1.4
3,314,302 - nusA b3169 0.16 +0.6
3,314,302 - nusA b3169 0.16 -0.8

Or see this region's nucleotide sequence